@article { author = {Alrefaie, Zienab}, title = {Increased Synaptophysin in the Prefrontal Cortex of Ovarectomized Rats Showing Depressive-like Behavior}, journal = {Bulletin of Egyptian Society for Physiological Sciences}, volume = {33}, number = {2}, pages = {1-12}, year = {2013}, publisher = {Egyptian Society for Physiological Sciences}, issn = {1110-0842}, eissn = {2356-9514}, doi = {10.21608/besps.2013.35205}, abstract = {Aim: Synaptophysin is an important synaptic marker. Changes in synaptophysin expression in response to ovarian hormones withdrawal have not been much investigated. The present study aimed to assess the prefrontal cortex (PFC) level of synaptophysin and its possible association with depressive-like behavior in ovarectomized rats.  Methods: Twinty female Wistar rats were included into sham-operated control group and ovarectomized group. 16 weeks following the surgical procedures, rats were tested for depression using the forced swim test (FST) and the tail suspension test (TST). Animals were sacrificed under diethyl ether anesthesia and prefrontal cortices were dissected and used for measurement of synaptophysin, brain derived neurotrophic factor (BDNF), nerve growth factor (NGF) and soluble amyloid beta (Aβ1-42). Results: The present data revealed a significant increase in immobility time in both FST and TST in ovarectomized rats. The PFC of ovarectomized rats exhibited a significant increase in synaptophysin and Aβ1-42, while both BDNF and NGF expression showed significant decrease. Conclusion: The present study suggests that the increase in PFC level of synaptophysin could be among mechanisms that underlie the depressive-like behavior demonstrated in ovarectomized rats through enhancement of glutamate release and subsequent glutamate neurotoxicity. }, keywords = {Ovariectomy,depression,Synaptophysin,BDNF,Aβ1-42}, url = {https://besps.journals.ekb.eg/article_35205.html}, eprint = {https://besps.journals.ekb.eg/article_35205_b6052fee32ec8d0ee5858f5377f9d400.pdf} } @article { author = {Alrefaie, Zienab}, title = {Effect of vitamin D3 Supplementation on Thyroid Hormones and Deiodinase 2 Expression in Streptozotocin-induced Diabetic Rats}, journal = {Bulletin of Egyptian Society for Physiological Sciences}, volume = {33}, number = {2}, pages = {13-22}, year = {2013}, publisher = {Egyptian Society for Physiological Sciences}, issn = {1110-0842}, eissn = {2356-9514}, doi = {10.21608/besps.2013.35211}, abstract = {Aim: Effect of vitamin D supplementation on peripheral conversion of T4 into T3 in diabetic rats has not been previously investigated. The present study aimed to assess the effect of vitamin D3 administration on deiodinase 2 (D2) expression in diabetic rats. Methods: Thirty male Wistar rats (200-250 g) were included into; control, diabetic and diabetic supplemented with vitamin D3 groups. Diabetes was induced by single intraperitoneal injection of streptozotocin 45 mg/kg in citrate buffer. Vitamin D3 was administered orally in a dose of 500 IU/kg/day in corn oil for 10 weeks. Serum levels of free T4, free T3 and TSH were measured. Tissue homogenates from liver, kidney, muscle, femur bone, heart and brain were obtained and assessed for expression of D2 mRNA. Results: Diabetic rats demonstrated significant increase in free T4 and significant decrease in free T3 together with non significant increase in TSH level compared to control rats. However the changes in thyroid profile were ameliorated by vitamin D3 administration to diabetic rats. D2 mRNA expression was significantly reduced in all tissue homogenates obtained from diabetic rats compared to control rats. Vitamin D3 treated diabetic rats showed significantly enhanced D2 mRNA expression in liver and brain homogenates, while in remaining tissues; the increase in D2 mRNA did not reach significance. Conclusion: Diabetes mellitus inhibited peripheral conversion of T4 into T3 secondary to reduction in the expression of D2 in almost all body tissues. Vitamin D3 administration to diabetic rats greatly corrected the alterations in thyroid profile and D2 expression. The present results point to the possible beneficial role of vitamin D3 against the subclinical hypothyroid state that could associate diabetes mellitus.}, keywords = {Diabetes mellitus,vitamin D3,thyroid hormones,deiodinase 2}, url = {https://besps.journals.ekb.eg/article_35211.html}, eprint = {https://besps.journals.ekb.eg/article_35211_21cefd2cba1a59b4d36bbae36cadeed0.pdf} } @article { author = {Ahmed, Nagwa and Zakhary, Madeha and Yousef, Reda and shaban, Marwa}, title = {Oxidative Stress, Angiogenesis and Apoptosis in Relation to Aging}, journal = {Bulletin of Egyptian Society for Physiological Sciences}, volume = {33}, number = {2}, pages = {23-36}, year = {2013}, publisher = {Egyptian Society for Physiological Sciences}, issn = {1110-0842}, eissn = {2356-9514}, doi = {10.21608/besps.2013.35215}, abstract = {Apoptosis and aging are complementary and cooperative processes that reduce cell proliferation and promote resistance to tumor development. Cellular levels of oxidative stress increase during physiological aging and tend to induce apoptosis, thereby influencing cellular and tissue aging and life span. Aim of study: is to determine the relationship between aging and apoptotic indices in the form of s-Fas and oxidant-antioxidant axis by determination of Nitric oxide (NO) and Lipid peroxide (LP). Also, the role of angiogenesis by determination of PD/ECGF (Thymidine phosphorylase) is another aim of the present work. Subjects and Methods: Fasting serum samples from 50 healthy individuals divided into five groups with age range between 20-29; 30-39; 40-49; 50-59 and 60-69 from those who proved to be in a normal state of health and free from any signs of chronic diseases. Serum levels of NO, LP and thymidine phosphorylase were measured by chemical methods, while s-Fas was assayed by ELIZA kit. Results: There were significant higher levels of serum NO, lipid peroxide, s-Fas and thymidine phosphorylase in older persons than in young ones, and a significant positive correlation between NO, lipid peroxide, s-Fas and thymidine phosphorylase when each was correlated with age. Conclusion: it could be concluded that oxidative stress, apoptosis and angiogenic factor increase with aging and may play an important role in its pathogenesis. The question remains whether they are the initial pathogenic events of aging or might be a consequence of it.}, keywords = {aging,Angiogenesis,Oxidative Stress,apoptosis}, url = {https://besps.journals.ekb.eg/article_35215.html}, eprint = {https://besps.journals.ekb.eg/article_35215_3125cf9afc843424bd2c94868cdd5cf1.pdf} } @article { author = {Soliman, Nema and Shafik, Noha and Shoheib, Zeinab and Ashour, Dalia}, title = {Expression of Some Pro-inflammatory Cytokines and Inflammatory Mediators in Experimental Model of Trichinella Spiralis}, journal = {Bulletin of Egyptian Society for Physiological Sciences}, volume = {33}, number = {2}, pages = {37-60}, year = {2013}, publisher = {Egyptian Society for Physiological Sciences}, issn = {1110-0842}, eissn = {2356-9514}, doi = {10.21608/besps.2013.35220}, abstract = {Trichinellosis is a zoonosis acquired by the ingestion of undercooked meat containing the infective larvae of Trichinella spiralis (T. spiralis). Despite veterinary public health efforts to control it, re-emergence of the disease has been observed in many areas of the world in the past 10–20 years. Although ivermectin (IVM) achieved high efficacy as larvicidal in trichinellosis, the possibility of disease transmission to other hosts is still present that will hinder the eradication of that serious disease. Therefore, our goal is to determine the efficacy of IVM on the subsequent infectivity of T. spiralis larvae in experimental animals through parasitological and biochemical studies of the intestinal phase. We found that the proinflammatory cytokine; interleukin-1β (IL-1β) and epithelial neutrophil activating peptide 78 (ENA-78) mRNA expression were decreased in group II which was infected by IVM- treated T. spiralis larvae on the 5th day post infection, also the same group showed the lowest levels of vasoactive intestinal peptide (VIP) and protein carbonyl (PCO) as well as the lowest activities of myeloperoxidase (MPO) and superoxide dismutase (SOD) enzymes. The lowest adult and larval counts were observed in group II at the 5 th day post infection with highly significant differences in comparison to other groups. In conclusion, IVM appears to affect the infectivity of T. spiralis larvae with subsequent decrease of T. spiralis adults and total larval count. These effects were more pronounced when IVM was given early in the infection. IVM is feasible and useful drug in the treatment of current infection and control of subsequent infections with T. spiralis.}, keywords = {Trichinella spiralis,ivermectin (IVM),larval infectivity,Proinflammatory cytokines,vasoactive intestinal peptide (VIP),protein carbonyl (PCO),superoxide dismutase (SOD)}, url = {https://besps.journals.ekb.eg/article_35220.html}, eprint = {https://besps.journals.ekb.eg/article_35220_9e67a58a44d85e8dbe855557749cacf3.pdf} } @article { author = {Abdelmoaty, Mohamed and Bogdady, Ahmed and Attia, Mervat}, title = {Serum Homocysteine and MTHFR C677T and A1298C gene polymorphisms in Type 2 Diabetic Patients with Nephropathy in Sector of Egyptian Population}, journal = {Bulletin of Egyptian Society for Physiological Sciences}, volume = {33}, number = {2}, pages = {61-72}, year = {2013}, publisher = {Egyptian Society for Physiological Sciences}, issn = {1110-0842}, eissn = {2356-9514}, doi = {10.21608/besps.2013.35224}, abstract = {Homocysteine (Hcy) is an amino acid, which is a product of methionine demethylation and a precursor to cysteine biosynthesis. Elevations in plasma Hcy (homocysteinemia) are frequently found in increased risk of atherosclerotic, coronary artery disease (CAD), venous thrombosis and stroke. Hcy injuries the endothelium and may have a role in microvascular complication of type 2 diabetes mellitus (T2DM). Two common mutations in the 5,10-methylenetetrahydrofolate reductase (MTHFR) gene (MTHFR C677T and A1298C) result in elevated Hcy levels. The aim of the present study was to investigate the relationship between plasma Hcy levels and diabetic nephropathy (DN). Serum Hcy levels, MTHFR genotype, and a panel ofvariables were evaluated in a sample of 75 T2DM patients with DN, 55 patients without nephropathy and also 95 non-diabetic control Egyptian subjects. Its common genetic polymorphisms (MTHFR C677T and A1298C) were determined for these patients and control subjects together with their correlation with changes in Hcy levels. Biochemical variables (including 24 hours albuminuria, GFR and serum total Hcy, lipogram and HbA1c beside blood urea and serum creatinine) and lifestyle characteristics were investigated. MTHFR genotype was studied by PCR-RFLP analysis, and total Hcy levels were measured by ELISA. The plasma Hcy levels were significantly higher in the diabetic nephropathy (19.8± 2.3 μmol/L) than uncomplicated type 2 diabetic patients (12.7± 2.1 μmol/L, P<0.05) and also, the control subjects (11.8±1.8μmol/L, P<0.05). There were no differences between uncomplicated diabetic patients and control subjects with respect to Hcy levels. C677T and T677T were highly prevalent among DN patients, with frequencies of 0.40 and 0.36 respectively. C677T, but not A1298C, SNP, is a risk factor for DN, presumably through elevating serum Hcy level.}, keywords = {diabetic nephropathy,homocysteine, methylenetetrahydrofolate reductase, type 2diabetes}, url = {https://besps.journals.ekb.eg/article_35224.html}, eprint = {https://besps.journals.ekb.eg/article_35224_7860620855bad5c0d951df7eb2b68b0a.pdf} } @article { author = {El-Dahtory, Faeza and Abdel Aziz, A}, title = {Some Hormonal and Biochemical Disorders in Patients of Down's syndrome in Egypt}, journal = {Bulletin of Egyptian Society for Physiological Sciences}, volume = {33}, number = {2}, pages = {73-82}, year = {2013}, publisher = {Egyptian Society for Physiological Sciences}, issn = {1110-0842}, eissn = {2356-9514}, doi = {10.21608/besps.2013.35226}, abstract = {Down syndrome (DS) is caused by an extra chromosome 21 and is the most common autosomal chromosome aberration. Down syndrome results in mental retardation and several congenital malformations. The aim: The purpose of this study is to shed light on the importance of follow-up screening analysis of some hormones and some biochemical parameters in patients with Down syndrome. Methods: Laboratory variables in 31 adult patients with Down's syndrome were compared with those of 30 matched controls. Fasting blood samples were collected for estimation of free thyroxin (FT4), thyroid stimulating hormones (TSH), follicle-stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E2), free testosterone (T), as well as serum levels of creatinine, uric acid and albumin. Results: The thyroid function tests documented a higher mean TSH and lower FT4 mean levels in Down's syndrome than in controls. FSH and LH mean levels were significantly higher in DS group than in controls (p<0.05). The mean level of estradiol (E2) in females with DS (11.61±2.28 pg/ml) was significantly lower than controls (31.18±2.71 pg/ml) (p<0.05), while the level of free testosterone (fT) was significantly higher compared with control subjects (2.71±0.66 pg/ml versus 1.2±0.37 pg/ml respectively, p<0.05. As well as, in males DS patients, the level of E2 was normal (11.33±1.04 pg/ml) while the level of free testosterone was significantly lower compared with controls (1.98±0.21 pg/ml) and (2.63± 0.44 pg/ml) respectively. Also, levels of serum creatinine and uric acid in DS patients were higher compared with controls, while the level of albumin was low (p<0.05). Conclusion: Through the results of measuring some hormones and biochemical changes in this study of Down syndrome patients compared to healthy subjects, it turns out DS patients are more vulnerable to diseases than others, such as renal and hepatic diseases, infertility and should be followed to keep under control.}, keywords = {Down syndrome. fT4,FSH,E2,LH,free testosterone,albumin,Kidney function}, url = {https://besps.journals.ekb.eg/article_35226.html}, eprint = {https://besps.journals.ekb.eg/article_35226_f65aa76ca4b3c2607d5dc71104e4f353.pdf} } @article { author = {Ahmed, Nagwa and Abdelmoaty, Mohamed and Abd. Elraheem, Omar}, title = {Nitric Oxide, Lipid Peroxiode and Manganese Superoxide Dismutase with Its Genetic Polymorphism in Breast Cancer and Benign Breast Patients among Egyptian Population Sample}, journal = {Bulletin of Egyptian Society for Physiological Sciences}, volume = {33}, number = {2}, pages = {83-96}, year = {2013}, publisher = {Egyptian Society for Physiological Sciences}, issn = {1110-0842}, eissn = {2356-9514}, doi = {10.21608/besps.2013.35228}, abstract = {Breast cancers are potentially life threatening malignancies in women. Current evidence indicates that free radicals and mitochondrial DNA damage play a prominent role in the development of breast cancer. Manganese superoxide dismutase (MnSOD) is a major enzyme that is responsible for the detoxification of reactive oxygen species (ROS) in the mitochondria. One of the several metabolic pathways involved in breast carcinogenesis is the human polymorphism in the mitochondria targeting sequence Ala9Val of the MnSOD gene. It is hypothesized that the valine to alanine substitution seems to alter transport of the enzyme into the mitochondrion, changing its efficacy in fighting oxidative stress. The present study included 24 females with breast cancer, 27females with benign breast lesion and 23 female healthy controls. Whole blood samples were collected, part on heparin for DNA extraction which was used to assay MnSOD polymorphism by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. The other part of blood samples was collected on plain tunes and serum together with breast tissue samples were used for estimation of lipid peroxides (LP), nitric oxide (NO) and total superoxide dismutase (SOD) activity. The results of the study showed significant elevations of serum NO mean levels in breast cancer patients (53.57 ±10.6 μmol/l) compared to both control subjects (25.4 ±8.3 μmol/l) and patients with benign breast lesions (23.6 ±7.8 μmol/l), the latter mean level is not significantly different from that of controls. NO levels in breast cancer tissues (7.3±1.3 pmol /mg protein) were significantly higher compared to both control subjects (1.6 ±0.03 pmol /mg protein) and patients with benign breast lesions (3.3±0.7 pmol/mg protein), Also, the latter mean level is significantly higher compared to that of controls. Serum MDA levels in breast cancer patients (1.58 ±104 μmol/l) were not significantly different compared to both control subjects (0.9 ±0.7 μmol/l) and patients with benign breast lesions (1.07 ±0.62 μmol/l), MDA mean levels in breast cancer tissues (244.2±23.1 pmol /mg protein) were significantly higher compared to both control subjects (77.4 ±8.3 pmole /mg protein) and patients with benign breast lesions (91.2±91.2 pmole /mg protein), , the latter mean level is not significantly different from that of controls. Serum total SOD mean activity of breast cancer patients (145.4 ±24.6 U/l) was significantly lower compared to both control subjects (231.5 ±39.4 U/l) and patients  with benign breast lesions (211.1 ±37.4 U/l), , the latter mean level is not significantly different from that of controls. Total SOD mean activity in breast cancer tissues (9.3±2.7 U/mg protein) were significantly lower compared to both control subjects (25.5 ±1.1 U/mg protein) and patients with benign breast lesions (23.1±2.3 U/mg protein), , the latter mean activity is not significantly different from that of controls.Ala/Val genotype is the most prominent in the breast cancer women (66.7%) with odd ratio =2.63 (p<0.03) as compared to the Val/Val which was higher in control (69.6%).Conclusion: It could be concluded that the individual susceptibility to breast cancer may be modulated by MnSOD polymorphism, and the combination of genetic factors with the increase of the free radicals that induce lipid peroxidation decreasing activity of enzymatic antioxidants might play an important role in the pathogenesis of breast cancer.}, keywords = {Nitric oxide,Polymorphism,Lipid peroxide,breast cancer}, url = {https://besps.journals.ekb.eg/article_35228.html}, eprint = {https://besps.journals.ekb.eg/article_35228_8ba400f7d6e1df9e9b85442a85effb78.pdf} } @article { author = {Abdel Aziz, A and El-Dahtory, Faeza and Salah, Nivin and El-Sallab, Bossy}, title = {Role of Interleukin-6 and Oxidative Stress in Mentally Retarded patients}, journal = {Bulletin of Egyptian Society for Physiological Sciences}, volume = {33}, number = {2}, pages = {97-112}, year = {2013}, publisher = {Egyptian Society for Physiological Sciences}, issn = {1110-0842}, eissn = {2356-9514}, doi = {10.21608/besps.2013.35232}, abstract = {Abnormalities in thyroid state may result in mental retardation. Oxidative stress and elevated serum inerleukin-6 (IL-6) levels have been identified as an important contributor to neurodegeneration. The aim: This work has been carried out to evaluate the interleukin-6, thyroid hormones as well as some parameters related to oxidative stress in mentally retarded patients with chromosomal abnormalities. Methods: karyotyping for blood, serum thyroid stimulating hormone (TSH), free thyroxin (FT4), superoxide dismutase (SOD), catalase(CAT), malondialdehyde (MDA) and interleukin-6 (IL-6) were measured in 60 mentally retarded patients and 30 healthy volunteers included as control groups. Results: Chromosomal abnormalities were studied in 60 mentally retarded patients, 50 of them were Down's syndromes and the other 10 consisted of (translocations, trisomies, ring chromosome, Edward’s, Patau and Klinefelter syndromes). Thirty three subjects were euthyroid and 27 subjects were hypothyroid. In euthyroid group there was no significant difference in the values of TSH, FT4 and SOD whereas the values of MDA and CAT were significantly higher compared with control. On the other hand, IL-6 levels were highly significantly increased compared with the control group. In hypothyroid group there was a highly significant increase in TSH and IL-6 levels and a highly significant decrease in SOD, CAT, MDA and FT4 levels compared with those of the control group. Conclusion: Increased oxidative stress and IL-6 as well as hypothyroidism are common in mentally retarded individuals with chromosomal abnormalities. These findings are useful in supporting future antioxidant therapies and chemical inhibitors against the function of IL-6 to improve those patients.}, keywords = {Chromosomal abnormalities,Interleukin-6,Mentally retarded patients,Oxidative Stress,thyroid hormones}, url = {https://besps.journals.ekb.eg/article_35232.html}, eprint = {https://besps.journals.ekb.eg/article_35232_841e907dd5e40d0c61dcda7ae0300560.pdf} } @article { author = {Seleem, Amal and Settin, Ahmad and El-Kholy, Wafaa and Fathy, Hend and Mohamed, Afaf}, title = {TNF-α (-308) G>A and IFN-γ (+874) A>T gene Polymorphisms in Egyptian Patients with Systemic Lupus Erythematosus: Focus on Lupus Nephritis.}, journal = {Bulletin of Egyptian Society for Physiological Sciences}, volume = {33}, number = {2}, pages = {113-126}, year = {2013}, publisher = {Egyptian Society for Physiological Sciences}, issn = {1110-0842}, eissn = {2356-9514}, doi = {10.21608/besps.2013.35240}, abstract = {Lupus nephritis (LN) remains one of the most serious manifestations of systemic lupus erythematosus (SLE) associated with considerable morbidity and mortality. This study was planned to test for the association of TNF-α -308 G>A and IFN-γ +874 A>T gene polymorphisms with susceptibility of LN in Egyptian patients. Subjects and Methods: A cross-sectional study was conducted on 125 patients (72 females and 53 males) suffering from SLE having LN. Control group consisted of 112 age matched healthy (46 males and 66 females) individuals. For all patients, characterization of TNF-α -308 G>A and IFN-γ +874 A>T genetic polymorphisms was done using the allele-specific PCR technique. Results Regarding TNF-α -308 G>A, the A allele was associated with a significantly elevated odd ratio (OR) of 3.2 (95% confidence interval (CI) =2.15 -5.01, P = 0.003) in SLE cases associated with LN. The AA genotype, was statistically insignificant (95% CI = 0.61-46.5, P 0.12) increased risk. Combined genotypes (AA plus GA) versus GG was associated with a significant increase in SLE having nephritis compared to controls (OR = 2.51, 95% CI = 1.26-4.99, p=0.009). Regarding the IFN-γ +874, the T allele of this gene was not associated with increased risk of SLE having nephritis (OR 1.43, 95% CI =0.93-2.20, p 0.13). Genotype AT was associated with increased association of SLE having nephritis (OR 2.06, CI= 3.62-1.18, p = 0.01). In Conclusions: Polymorphisms related to TNF-α -308 G>A and IFN-γ +874 A>T genes may be considered as genetic risk factors for SLE having lupus nephritis.}, keywords = {SLE,Cytokines,Gene polymorphism,TNF-α -308 G>A,IFN-γ +874 A>T}, url = {https://besps.journals.ekb.eg/article_35240.html}, eprint = {https://besps.journals.ekb.eg/article_35240_64c3eaed8a8cb0050a67f039becdc43c.pdf} } @article { author = {Abdel Aziza, MT and Rizk, Ameen and Ahmed, HH and Rashed, LA and Sohier, M and Talaat, Ahmed and ALKaffas, MA}, title = {The Anti-Inflammatory Mechanism of Action of a Water Soluble Curcumin Derivative and Mscs in Alzheimer Disease Induced In Rats}, journal = {Bulletin of Egyptian Society for Physiological Sciences}, volume = {33}, number = {2}, pages = {127-144}, year = {2013}, publisher = {Egyptian Society for Physiological Sciences}, issn = {1110-0842}, eissn = {2356-9514}, doi = {10.21608/besps.2013.35244}, abstract = {The objective is to evaluate the possible mechanisms of action of a water soluble curcumin derivative and MSCs in Alzheimer disease (AD). Materials and Methods: This work included: Seventy rats which were divided equally into: control group, AD group (induced by lipopolysaccharide), AD group received a novel curcumin derivative (NCD), AD group received pure curcumin, AD group received MSCs then NCD, AD group received MSCs, AD group received MSCs with NCD. Histopathological examination and estimation of serum IL-10 and IL-6 by ELISA, estimation of MDA and GSH in brain tissue by colorimetry and estimation of tissue cholesterol were performed. Results: Histopathological examination of brain tissue from AD animals revealed the presence of plaques. Administration of MSCs or MSCs with NCD into rats after induction of experimental AD improved the histopathological picture with plaque disappearance, while other groups showed decrease of plaque formation. Levels of IL-10 were increased while of IL-6 were decreased in all treated groups. MDA and cholesterol levels were decreased while GSH levels were increased in all treated groups.Conclusion: Administration of BM-derived MSCs either alone or with the NCD exert a therapeutic effect on the brain lesion in Alzheimer’s disease. This effect may be through the anti-inflammatory and antioxidant action of both MSCs and curcumin. These data suggests that MSCs and curcumin may be a therapeutic target in treatment of AD.}, keywords = {Alzheimer disease,Mesenchymal Stem Cells,Novel curcumin derivative}, url = {https://besps.journals.ekb.eg/article_35244.html}, eprint = {https://besps.journals.ekb.eg/article_35244_a31828a3cceb7f01bcf36c62c2169e61.pdf} } @article { author = {Abdel Aziz, MT and Rizk, Ameen and Ahmed, HH and Rashed, LA and Sohier, M and Talaat, Ahmed and ALKaffas, MA}, title = {The Anti-Apoptotic Mechanism of Action of a Water Soluble Curcumin Derivative and Mscs in Alzheimer Disease Induced In Rats}, journal = {Bulletin of Egyptian Society for Physiological Sciences}, volume = {33}, number = {2}, pages = {145-160}, year = {2013}, publisher = {Egyptian Society for Physiological Sciences}, issn = {1110-0842}, eissn = {2356-9514}, doi = {10.21608/besps.2013.35249}, abstract = {The objective is to evaluate the possible mechanisms of action of a water soluble curcumin derivative and MSCs in Alzheimer disease (AD). Materials and Methods: This work included: Seventy rats which were divided equally into: control group, AD group (induced by lipopolysaccharide), AD group received a novel curcumin derivative (NCD), AD group received pure curcumin, AD group received MSCs then NCD, AD group received MSCs, AD group received MSCs with NCD. Histopathological examination and gene expression of Bax, bcl2 and Seladin-1 gene expression in brain tissue and estimation of MDA and GSH in brain tissue by colorimetry were performed. Results: Histopathological examination of brain tissue from AD animals revealed the presence of plaques. Administration of MSCs or MSCs with NCD into rats after induction of experimental AD improved the histopathological picture with plaque disappearance and improved the behavior study, while other groups showed decrease of plaque formation. Gene expression demonstrated that bcl2 and Seladin-1 were upregulated while Bax gene was downregulated in all treated groups with a significant effect in the group treated with MSCs alone and group treated with both MSCs and NCD. MDA levels were decreased while GSH levels were increased in all treated groups. Conclusion: Administration of BM-derived MSCs either alone or with the NCD exerts a therapeutic effect on the brain lesion in Alzheimer’s disease. This effect may be through the antioxidant and antiapoptotic action of both MSCs and curcumin. These data suggests that MSCs and curcumin may be a therapeutic target in treatment of AD.}, keywords = {Alzheimer disease,Mesenchymal Stem Cells,Novel curcumin derivative}, url = {https://besps.journals.ekb.eg/article_35249.html}, eprint = {https://besps.journals.ekb.eg/article_35249_b82092c760ff3be33bd59469a4b95e05.pdf} } @article { author = {Gadalla, A and Al Gendy, A and Hashim, A and Amer, M and Sabry, O}, title = {Effects of Immobilization Stress and Adrenomodullin on Interleukin-10 Levels in Some Rat Tissues}, journal = {Bulletin of Egyptian Society for Physiological Sciences}, volume = {33}, number = {2}, pages = {161-171}, year = {2013}, publisher = {Egyptian Society for Physiological Sciences}, issn = {1110-0842}, eissn = {2356-9514}, doi = {10.21608/besps.2013.35257}, abstract = {Background: Immobilization stress known to stimulate sympathetic activity, as well as the hypothalamic–pituitary–adrenal axis (HPA), produces a significant increase in adrenomedullin (AdM) levels, suggesting a regulatory or protective role for AdM in countering HPA activation that follows a variety of stressors. Stressors can modulate the secretion of proinflammatory cytokines. Interleukin (IL)-10 is a potent activator of the HPA and appears to play a pathogenic role in conditions related to stress Objective: The aim of this study was to evaluate and validate the effects of AdM administration and immobilization stress treatment on IL-10 levels in rat liver, lung, brain and heart tissues. Materials and Methods: The study was carried out on twenty-four male albino rats (8 months old, 190–240 g). The animals were divided into 4 groups of 6 rats each group. Group A : control group. Group B : AdM-treated group , rats received intraperitonealy (i.p.) injection of AdM (2000 μg/g body weight) once daily for 1 week. Group C : immobilized stress group ,(rats were immobilized by keeping them into transparent plastic jars with 5 holes for 4hrs per day for 1 week).Group D: immobilized stress +AdM group. Rats were immobilized by keeping them into transparent plastic jars with 5 holes for 4hrs a day for 1 week and were given AdM i.p at a single dose of 2000 μg/g body weight for a week. At the end of the experiment, the concentration of IL-10 was determined using an enzyme-linked immunosorbent assay (ELISA) kit. Results: The results of the present study showed that IL-10 levels increased in all tissues in immobilized stress group when compared to control , also IL-10 levels were increased in AdM treatment group in all tissues when compared to control . IL-10 levels were decreased in the immobilization stress + AdM treatment group in all tissues when compared to the stress group, increased IL-10 levels in brain and lung tissue but decreased in heart and liver tissues in the immobilization stress + AdM treatment group when compared to the AdM treatment group. Conclusion: The results suggest that immobilization stress may induce increase of rat proinflammatory cytokine IL-10 and AdM may play a regulatory or protective role for immobilization stress.}, keywords = {Immobilization stress,Adrenomedullin,Interleukin 10}, url = {https://besps.journals.ekb.eg/article_35257.html}, eprint = {https://besps.journals.ekb.eg/article_35257_675a3212b125110bcfdf713517e49a05.pdf} }