eng
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
2017-12-01
37
2
92
109
10.21608/besps.2017.8231
8231
Original Article
Possible Protective Role of Erythropoietin in Diabetic Cardiomyopathy in Rats
Mohammed Rabei
1
Hanaa Abd-Elmoniem
2
Mohammed Sarhan
3
Nisreen Abo-Elmaaty
nisreenomar@hotmail.com
4
Lashin Ali
5
Department of Medical Physiology,Faculty of Medicine,Mansoura University, Mansoura, Egypt.
Department of Medical Physiology, Faculty of Medicine,Mansoura University, Mansoura, Egypt.
Department of Medical Physiology, Faculty of Medicine,Mansoura University, Mansoura, Egypt.
Department of Medical Physiology,Faculty of Medicine,Mansoura University, Mansoura, Egypt.
Department of Medical Physiology, Faculty of Medicine,Mansoura University, Mansoura, Egypt.
There are controversial experimental works about the way of EPO in protection againstDCM; whether EPO action is through making better metabolic condition and glycemiccontrol or the antioxidant and antiapoptotic effect. So ,the aim of this study was to evaluatethe modulating effects of EPO on some biochemical markers in diabetic cardiomyopathy.Fifty male Sprague Dawley rats were divided into five groups of 10rats each. The rats ofgroup Ⅰ served as normal control. Group Ⅱ received HFD for 1month and STZ in aconcentration of 35mg/kg intraperitoneal. Group Ⅲ:(Diab + Gliben) diabetic rats treatedwith glibenclamide, Gliben was given at a dose (0.6 mg/kg orally) for 4 weeks, Group Ⅳ:Diabetic rats treated with EPO; twice a week 4 weeks' duration. (1,000 IU/kg) and Group Ⅴ:diabetic rats treated by both (EPO + Gliben) with the same previous doses for 4 weeks'duration. At the end of experiment serum samples and cardiac tissues were obtained from thesacrificed rats. Cardiac enzymes, blood glucose levels, serum insulin level and oxidativestress markers were estimated. Diabetic cardiomyopathy changes were confirmed byelectrophysiological and histopathological results as well as increased serum activity o fcardiac enzymes and high blood glucose levels. EPO exhibited myocardial protection in ratswith DCM, at least in part through antioxidant effect and better glycemic control. However,this study was based on animal models in vivo, and detailed molecular mechanisms should befurther investigated using in vitro studies. Clinical trials may also be considered to validatethe results in humans, following further pre-clinical studies.
https://besps.journals.ekb.eg/article_8231_f08c4fcfe405841692c9ef7dd878162d.pdf
Erythropoietin (EPO)
Diabetic cardiomyopathy
Glibenclamide
Oxidative Stress
High Fat Diet (HFD)
eng
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
2017-12-01
37
2
110
128
10.21608/besps.2017.8232
8232
Original Article
Potential Molecular Biomarkers for Prediction of Coronary Artery Disease, its Severity and Extent
Naglaa Azab
naglaa.azab@fmed.bu.edu.eg
1
Said Tawfik
2
Department of Medical Biochemistry, Faculty of Medicine, Benha University, Benha - Egypt.
Department of Cardiovascular medicine, Faculty of Medicine, Benha University, Benha - Egypt.
Coronary artery disease (CAD) has high morbidity and mortality, therefore accurate detection of CAD and atherosclerotic plaque burden is essential. Invasive coronary angiography (ICA) can confirm the presence of luminal stenosis caused by atherosclerotic plaque and is an important tool for the quantification of CAD plaque burden. However, it has many limitations. Therefore, new methods for accurate noninvasive diagnosis of CAD using genetic markers were exerted. This study aimed at evaluation of new genetic biomarkers in diagnosing CAD and determining its severity and extent, using Gensini score as a scoring model. Sixty two subjects who had undergone coronary angiography and analyzed for the presence, severity and extent of CAD using Gensini score were selected for this study. Twenty subjects had normal Gensini score, 24 subjects were with mild to moderate CAD and 18 subjects were with severe CAD. Extraction of total RNA from whole blood was done together with PCR amplification of PNPLA2, TUBA3, FTL and AHR genes and the house keeping gene GAPDH. It was found that PNPLA2 and TUBA3 were significantly lower in CAD patients and mild/moderate CAD than in controls. However, PNPLA2 was significantly higher in severe CAD than in mild/moderate CAD. Moreover, PNPLA2 gene had The highest specificity for CAD prediction of (100%), followed by TUBA3 (60%) then FTL and AHR (each 40%), and both PNPLA2 and FTL gene had the highest sensitivity for CAD diagnosis (80.95%), followed by TUBA 3 (76.19%), then AHR (71.43%). On the other hand, PNPLA2 showed the highest specificity in CAD severity prediction (83.33%), followed by TUBA3, FTL and AHR with equal specificity (each 50%), and TUBA 3 showed the highest sensitivity (66.67%) followed by PNPLA2,, FTL and AHR with equal sensitivity (each 55.56%). In conclusion, these genes are useful in prediction of CAD and CAD severity especially emphasizing PNPLA2 and TUBA3 and further studies on larger population number and other genes are recommended.
https://besps.journals.ekb.eg/article_8232_ffe256f25ae9641484fefccc0f55a8c0.pdf
molecular Biomarkers
Coronary artery disease (CAD)
plaque burden
eng
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
2017-12-01
37
2
129
142
10.21608/besps.2017.8243
8243
Original Article
Combined Estimation of Serum Procalcitonin Levels and Clinical Pulmonary Infection Score Improves Predictability for Survival of Ventilation-associated Pneumonia Patients
Adel Al-kholy
adeladel59@yahoo.com
1
Ahmed Salem
2
Maha Hagras
3
Department of Medical Biochemistry, Faculty of Medicine, Benha University, Egypt.
Department of Anesthesia & ICU, Faculty of Medicine, Tanta University, Egypt.
Department of Clinical Pathology, Faculty of Medicine, Tanta University, Egypt.
Objectives: To evaluate predictability of estimation of C-reactive protein (CRP) and procalcitonin (PCT) levels for diagnosis and survival of ventilator-associated pneumonia (VAP) patients. Patients & Methods: The study included 53 VAP patients and 37 No VAP patients who were assessed using Clinical Pulmonary Infection Score (CPIS) and Acute Physiology and Chronic Health Evaluation II (APACHE II) scores. Blood samples were collected on D0 and D4 for estimation of serum CRP and PCT levels. Patients were managed according to Surviving Sepsis Campaign guidelines. The 28-day mortality rate (MR) and the predictors for mortality were determined. Results: Total MR was 43.3% with significant difference between both groups. APACHE II and CPIS scores were significantly higher in non-survivors of both groups and in VAP than No VAP patients. Serum CRP and PCT levels were significantly higher in VAP patients and in non-survivors than survivors. Change of CRP level was significantly higher in No VAP than VAP survivors, while change of PCT levels was significantly higher in VAP survivors than non-survivors. High CPIS score and D0 PCT level, but low decreases of CRP and PCT levels are positive predictors for VAP diagnosis. High D4 PCT level and CPIS scores, but low decreases of PCT levels are significant predictors for mortality. Conclusion: VAP had high 28-day mortality rate. Combined evaluation of CPIS score and PCT levels improved the ability to diagnose VAP and low levels of both are independent predictors of survival.
https://besps.journals.ekb.eg/article_8243_937ce2f7a2b137774c5ff3c10bb2cf4b.pdf
Ventilator-Associated Pneumonia
C-reactive protein
Procacitonin
CPIS score
survival rate
eng
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
2017-12-01
37
2
143
153
10.21608/besps.2017.8244
8244
Original Article
ABCA1 gene polymorphism and oxidative stress in polycystic ovary syndrome: Role of antioxidant therapy for prevention and control
Rehan Monir
1
Abdelnaser Badawy
2
Naglaa Mokhtar
3
Hanan Nabil
4
Mohammed Alruwaili
5
Department of Medical Biochemistry,Faculty of Medicine,Mansoura University, Mansoura, Egypt.
Department of Medical Biochemistry,Faculty of Medicine,Mansoura University, Mansoura, Egypt.
Department of Medical Biochemistry, Faculty of Medicine,Mansoura University, Mansoura, Egypt.
Department of Obstetrics and Gynecology, Mansoura University Hospital, Mansoura, Egypt.
Department of Medical Laboratory Technology, Northern Border University, Faculty of Applied Medical Sciences, Arar, Saudi Arabia.
Polycystic ovary syndrome (PCOS) is a common health problem that affects women in childbearing age. Recent studies discussed the relation of oxidative stress with PCOS and suggested that the antioxidants may improve the prognosis of PCOS. Changes happened in ATP binding cassette transporter 1 (ABCA1) gene encoding a protein regulating entry and exit from cell membrane may contribute to dyslipidemia in patients with PCOS. The role of oxidative stress and ABCA1 gene polymorphism in the pathogenesis of PCOS was evaluated along with the effect of antioxidants in the prevention and treatment of this disease. The present study included 90 female patients with PCOS and 90 healthy women represented the control group. Patients with PCOS were further divided into two subgroups. The patients included in subgroup 1a were treated with antioxidant and hormonal therapy while patients included in subgroup 1b were treated with hormonal therapy only. ABCA1gene polymorphism and oxidative stress parameters were investigated in all groups. The level of the oxidant marker; MDA was higher in PCOS patients when compared to controls. In addition, the levels of GSH, vitamin E, ascorbic acid, and selenium were significantly decreased, while SOD and GPx activities were increased in PCOS patients when compared to controls. The response to treatment among PCOS patients was better in group 1a than group 1b. The frequency of ABCA1 AA genotype was higher in PCOS patients than the control group (32.2% and 11.1% respectively). It was concluded that oxidative stress may lead to PCOS which raises the possibility of using antioxidants for the prevention and control of PCOS. In addition, the polymorphism in ABCA1 gene may be associated with genetic susceptibility for PCOS.
https://besps.journals.ekb.eg/article_8244_8f9139c50ba1a07798a40551c99e9891.pdf
PCOs
Oxidative Stress
ABCA1 gene polymorphism
eng
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
2017-12-01
37
2
154
164
10.21608/besps.2017.8252
8252
Original Article
Assessment of Serum Level of Ghrelin in Patients with Chronic Obstructive Pulmonary Disease
Ramadan Bakr
1
Ibrahim El-Mahallawy
2
Mohammed Agha
3
Yasser El-Ghobashy
dryaser111@gmail.com
4
Mai Said
5
Chest Department, Faculty of Medicine, Menoufia University.
Chest Department, Faculty of Medicine, Menoufia University.
Chest Department, Faculty of Medicine, Menoufia University.
Medical Biochemistry Department, Faculty of Medicine, Menoufia University.
Menouf Chest Hospital
Objectives: to study the relationship between serum level of ghrelin and cachexia in patients with COPD. Background: Chronic obstructive pulmonary disease is a major cause of chronic morbidity and mortality throughout the world. COPD involves several systemic features. The link between ghrelin, cachexia and COPD needs to be clarified.Materials and methods: Serum levels of ghrelin were measured in 60 COPD patients (divided into 3 groups according to their BMI) and 30 control subjects using human ghrelin ELISA kits. Results: There was a significant increase in ghrelin level in COPD patients compared to control subjects. There was significant increase in serum ghrelin level in underweight COPD subgroup compared to normal weight and overweight subgroups. Serum levels of ghrelin in COPD patients were affected by the level of pulmonary obstruction. Conclusions: Serum level of ghrelin is increased in COPD patients, and is positively correlated with BMI and disease severity.
https://besps.journals.ekb.eg/article_8252_7161ba6e3dac1b6047aca71b2f9b93f7.pdf
ghrelin
COPD
Cachexia
eng
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
2017-12-01
37
2
165
175
10.21608/besps.2017.8258
8258
Original Article
Protective Effect of Copper (I)-Nicotinate Complex on DAB-Induced Hepatocellular Carcinoma in Rats
Mohamed Abdel-Mohsen
prof_abdelmohsenma@yahoo.com
1
Amany Youssef
2
Tarek El Sewedy
3
Morsy Abou-Youssef
4
Hala Maghraby
5
Ashgan El-Sarha
6
Mohammad Abd-Elrazak
7
Ahmed El-Gamal
8
Amr Dardeer
9
Department of Applied Medical Chemistry, Medical Research Institute, Alexandria University.
Department of Applied Medical Chemistry, Medical Research Institute, Alexandria University.
Department of Applied Medical Chemistry, Medical Research Institute, Alexandria University.
Department of Chemistry, Faculty of Science, Alexandria University.
Department of Pathology, Medical Research Institute, Alexandria University.
Department of Pathology, Medical Research Institute, Alexandria University.
Chemist, Medical Technology Center, Medical Research Institute, Alexandria University.
Chemist, Medical Technology Center, Medical Research Institute, Alexandria University.
Chemist, Medical Technology Center, Medical Research Institute, Alexandria University.
The aim of the present work was to evaluate the effect of copper (I)-nicotinate complex (CNC) on experimentally 4-Dimethylaminoazobenzene (DAB)-induced hepatocellular carcinoma (HCC) in male rats. In addition to the histopathological examination we measured hepatic glutathione content, malondialdehyde, nitric oxide levels, lamin B1 m-RNA, caspase-3 activity and serum interleukin-12 level after 2, 4, 6, 8 months from commencement of the experiment. Results: histopathological examination showed HCC development after 4 months of DAB administration. The hepatic glutathione content, lamin B1 m-RNA and nitric oxide levels were significantly elevated, while malondialdehyde, interlukin-12 levels and caspase-3 activity were significantly decreased with the progression and development of the HCC. On the other hand, administration of CNC one month before DAB delayed the development of neoplastic growth to the 8th month. Interestingly, when CNC was administered one month after DAB, it successfully prevented HCC development throughout the whole experiment as confirmed by histopathological data and explained by biochemical markers, as glutathione, lamin B-1 and nitric oxide were significantly declined but malondialdehyde, interlukin-12 and caspase-3 activity were significantly elevated compared to that in corresponding control group. Conclusion: CNC was able to delay or prevent HCC development in rats fed with the potent liver carcinogen DAB. Our data shows that CNC exerts its anti-tumour effects through modulating oxidative stress status as well as the machinery of apoptosis and angiogenesis. Therefore, CNC may be used as a potential protective anticancer agent.
https://besps.journals.ekb.eg/article_8258_e1fcde8f9909bc56e4c5abc7aabdc98f.pdf
Copper (I)-Nicotinate Complex
Hepatocellular carcinoma
Angiogenesis
apoptosis
Anticancer
eng
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
2017-12-01
37
2
176
188
10.21608/besps.2017.8263
8263
Original Article
Gestational Diabetes Mellitus And Leptin Gene polymorphism In Egyptian Women
Azza Abdu Allah
azza.abdallah@yahoo.com
1
Eman Abd El Gayed1
2
Alaa Abd El Gayed
3
Maha Desouky
4
Department of Medical Biochemistry, Faculty of Medicine and National Liver Institute, Menoufia University, Egypt.
Medical Biochemistry Department Faculty of Medicine, Menoufia University Egypt.
Obstetrics and Gynaecology Department Faculty of Medicine, Menoufia University Egypt.
Anatomy Department Minia University Egypt and Taibah University KSA.
Gestational diabetes is blood sugar elevation during pregnancy and exposes women to the risk for development of type 2 diabetes mellitus in later years of life. The LEP rs7799039 (2548G/A) polymorphism in the 5´ region of the LEP gene was reported not only to be associated with overweight but also to have a strong influence on leptin gene expression and adipose tissue secretion. The aim of this study is to evaluate the distribution of LEP rs7799039 (2548G/A) polymorphism and its plasma level in patients with gestational Diabetes Mellitus. This study was carried out on160 subjects divided into 2 groups; 80 patients with GDM (group I) and 80 healthy subjects served as controls (group II). All studied subjects were submitted to full history taking, general clinical examination and laboratory investigations including fasting and 2 hour post prandial blood glucose, total cholesterol (TC),triglycerides (TG), HDLc, LDLc, glycated hemoglobin (HbA1c), plasma leptin, fasting serum insulin, HOMA- IR and Genotyping of LEP rs 7799039 (2548G/A) polymorphism was analyzed by the TaqMan allelic Discrimination Assay Technique. Results showed significant statistical differences between the two studied groups regarding family history and BMI. Significant statistical difference between group (I) and group (II) regarding plasma leptin level (P value <0.001), serum insulin (P value <0.001), insulin resistance (P value <0.001) and LEP rs 7799039 (2548G/A) genotype distribution with increased frequency of the AG and AA genotype in patients with gestational diabetes (P value <0.001) and increased frequency of GG genotype in controls. Conclusion: This results indicate that leptin plays a role in increasing insulin sensitivity, LEP rs7799039 (2548G/A) and its plasma level can contribute to susceptibility to GDM and might be used for screening of the early detection of GDM.
https://besps.journals.ekb.eg/article_8263_768be084f4a1cdb3282f9e4b88d6b41b.pdf
Gestational diabetes
leptin
Gene polymorphism
eng
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
2017-12-01
37
2
189
198
10.21608/besps.2017.8264
8264
Original Article
Disturbed Inflammatory/Anti-inflammatory Milieu and Activation of Primary Phase Reactants may Underlie Development and Recurrence of First trimester Pregnancy Loss.
Mohamad Al-Sherbeny
1
Manal Hassaan
151239@o6u.edu.eg
2
Department of Obstetrics & Gynecology, Faculty of Medicine, Benha University.
Department of Medical Biochemistry, Faculty of Applied Medical Sciences, Oct.6 University.
Objectives: Estimation of serum levels of tumor necrosis factor-α (TNF-α), interleukin (IL-) 6 and 10 and alpha-1 antitrypsin (AAT) in pregnant women with current pregnancy loss (CPL) and non-pregnant women with history of recurrent PL (RPL) compared versus women with normal pregnancy (NP). Patients & Methods: All studied women underwent clinical and vaginal ultrasonographic examination for assurance of inclusion criteria and then gave blood samples for ELISA estimation of studied parameters. Results: Patients of CPL and RPL groups showed significantly high serum TNF-α and IL-6 levels with significantly lower serum IL-10 levels than women of NP group with significant difference between CPL and RPL groups. Serum AAT levels were significantly lower in CPL group than RPL and NP groups with significantly lower levels in RPL than NP group. High serum TNF-α and low AAT levels showed high positive predictive value (PPV) and sensitivity, respectively, for possibility of presence or recurrence of PL. Conclusion: Disturbed serum levels of pro- and anti-inflammatory cytokines could diagnose CPL with high PPV and persistence of their disturbed levels may predispose to RPL. Activation of primary phase reactant system may underlie, accompany or initiate PL, so low serum AAT during 1st trimester and its persistently low serum levels during inter-pregnancy intervals could be used as screening test for presence or liability for development of PL.
https://besps.journals.ekb.eg/article_8264_b1bdaf0f58db8030d5a9d78a9f0d46b5.pdf
First trimester pregnancy loss
Inflammatory cytokines
Alpha-1 antitrypsin
eng
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
2017-12-01
37
2
199
207
10.21608/besps.2017.8265
8265
Original Article
Cytokine assessment and Immunomodulatory Effect of Bee Venom in HBV&HCV Infected Patients
Helmy Ghoniemy
1
Shereen Mohamed
shereenkaddafy@yahoo.com
2
Hoda El Badya
3
Mohamed Abou- Zied
4
Hebat Allah Sayed Elsayh
5
Abir Elfiky
6
Departments of plant protection , Faculty of Agriculture , Fayoum University.
Departments of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Fayoum University.
Departments of internal medicine, Faculty of Medicine, Fayoum University.
Departments ofApiculture research, plant protectionsititue , A.R.C.
Departments ofApiculture research, plant protectionsititue , A.R.C.
Director of ANDI center of excellence in Antivenom research, RRD, VACSERA.
Background: Hepatitis B &Hepatitis C viral infections are common health problems worldwide. New strategies for treatment are now evolving. The therapeutic application of bee venom has been used in traditional medicine to treat many diseases. Aim: The aim of this study was to investigate the effect of bee-sting (venom) therapy on progression of chronic viral hepatitis B & C and on the levels of proinflammatory cytokines IL-1β , IL -2 and IL -6 and the anti inflammatory IL -10 in HBV&HCV infected patients. Methods: 67 Egyptian patients with chronic hepatitis were enrolled, 20 of them have HBV and 47 have HCV. Bee stings were administered using live bees at apipunctur point. CBC, liver enzymes, Hepatitis B &C RT-PCR, IL-1β, IL -2, IL -6 and IL -10 were estimated before & after bee stings therapy. Results: there was a significant decrease in ALT; AST (P≤0.05) after bee venom injection in both types of hepatitis. viral load was decreased during the course of treatment, it became negative after 9 months therapy in all cases of HBV infection and in 34.04% of HCV infection. IL-1β, IL -2, IL -6 and IL -10 levels were aalso significantly decreased after 9 months therapy in both types of hepatitis patients. Conclusions: bee venom stings decreased the viral load and IL-1β, IL -2, IL -6 and IL -10 after 9 month's therapy in both types of hepatitis.
https://besps.journals.ekb.eg/article_8265_d0d65d66d25584357b9a1f853c80c0a4.pdf
Bee venom
Hepatitis B virus
Hepatitis C virus
Cytokines
Bee venom therapy
eng
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
2017-12-01
37
2
208
226
10.21608/besps.2017.8268
8268
Original Article
Effects of Curcumin on Autophagy and Nrf2 signaling pathway in a Rat Model of High fructose diet induced Steatohepatitis
Soha Zakaria
soha.mahmoud@med.tanta.edu.eg
1
Maha ElBatsh
2
Shorouk Mowafy
3
Medical Biochemistry department, Faculty of Medicine, Tanta University, Egypt.
Clinical Pharmacology department, Faculty of Medicine, Menoufia University, Egypt.
Histopathology department, Faculty of Medicine, Tanta University, Egypt.
Increased fructose consumption predisposes to nonalcoholic steatohepatitis (NASH).There no effective treatment to be used as the second line when lifestyle modification is insufficient .So, the aim of this study was to evaluate the effect of Curcumin on some biochemical markers in fructose induced NASH . Sixty male albino rats were divided randomly and equally into three groups. Group I (control group). Group II (NASH group), received 70% fructose for 5 weeks .Group III (curcumin treated NASH group), fed as group II followed by daily administration of curcumin at a dose of 50 mg/kg orally for 6 weeks. Blood samples were collected to measure lipid profile, and liver enzymes activities . Liver tissues samples were collected for estimation of mRNA expression levels of nuclear factor E2-related factor-2 (Nrf2) using quantitative real-time PCR ,levels of Beclin 1, AMPK, IL6 and Nrf2 DNA-binding activity by ELISA method, and oxidative stress markers malondialdehyde (MDA), superoxide dismutase (SOD) ,GSH spectrophotometrically. NASH was confirmed by histopathology as well as increased activity of liver enzymes and marked dyslipidemia .Curcumin ameliorated biochemical disturbance in NASH by upregulating autophagy process through increasing Beclin 1, increasing AMPK and hampered IL6 levels in the liver . It also restored redox balance as evident by suppressing MDA formation and preservation of intracellular antioxidants status via increasing GSH, activating SOD and Nrf2 signaling pathway. Results suggested that curcumin has an effective role in counteracting both abnormalities in autophagy process and redox balance, supporting the potential utility of this natural product in effective treatment of NASH.
https://besps.journals.ekb.eg/article_8268_f197255a6ac88717743a5e92d87fc879.pdf
Curcumin
Nonalcoholic steatohepatitis(NASH) Beclin 1
Oxidative Stress
Nuclear factor erythroid 2-related factor-2 (Nrf2)
eng
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
2017-12-01
37
2
227
244
10.21608/besps.2017.8276
8276
Original Article
Combination Therapy with Quercetin and 5-Fluorouracil Ameliorates 1,2-Dimethylhydrazine Induced Carcinogenesis in the Colon of Wistar Rats.
Bakheet M. Elsadek
1
Mohamed Abdel Aziz
2
Sahar M. El-Deek
sahareldeek_123@yahoo.com
3
Mohamed M. Mahdy
4
Mahmoud Hussein
5
Department of Biochemistry, Faculty of Pharmacy, Al-Azhar University, Assiut Branch, P.O. Box No. 71524 Assiut, Egypt.
Department of Biochemistry, Faculty of Pharmacy, Al-Azhar University, Assiut Branch, P.O. Box No. 71524 Assiut, Egypt.
Department of Medical Biochemistry, Faculty of Medicine, Assiut University, P.O. Box No. 71526, Assiut, Egypt.
Department of Biochemistry, Faculty of Pharmacy, Al-Azhar University, Assiut Branch, P.O. Box No. 71524 Assiut, Egypt.
Department of Pathology, Faculty of Medicine, Assiut University, P.O. Box No. 71526, Assiut, Egypt.
Background: Colorectal carcinoma (CRC) is a common cause of cancer-related death worldwide. 5-fluorouracil (5-FU), a first-line chemotherapeutic drug in CRC, has several side effects limiting its therapeutic outcomes. Quercetin (QU) is a dietary bioflavonoid with antioxidant and cytotoxic prooxidant properties. Here, we hypothesize that combination treatment with QU and 5-FU can modulate 1,2-dimethylhydrazine (DMH) induced histological and biochemical changes in the colon of Wistar rats. Methods: A Wistar rats CRC model was established and the animals were randomly divided into five groups. Rats in group A received a suspending vehicle. Group B rats received DMH twice a week subcutaneously for 4 weeks. Animals in the other groups (C, D and E) received the same treatment of DMH, along with QU or 5-FU (individually) or combined QU+5-FU treatment. Results: The DMH-treated rats developed adverse histological alterations (aberrant crypt foci, ACF) and biochemical changes (elevated serum CA19-9; reduced tissue levels of enzymatic antioxidants; elevated CDH12 protein expression and decreased SOX7 mRNA levels). Treatment of DMH-treated animals with QU+5-FU (but not with QU or 5-FU, individually) significantly reversed these changes (i.e., suppressed the formation of ACF; decreased the CA19-9 levels; reduced CDH12 protein expression and increased SOX7 mRNA expression). Conclusions: Conclusively, to the best of our knowledge, our study was the first to evaluate the effects of QU+5-FU treatment on the histological and molecular changes following DMH administration in a rat colon model. Our data suggest that combination therapy with QU+5-FU has therapeutic benefits in colon cancer induced by DMH, with potential for translation to spontaneous disease.
https://besps.journals.ekb.eg/article_8276_33a8a7823e3c95b6054e178b9d47bd2c.pdf
5-Fluorouracil
CDH12
Colorectal carcinoma Dimethylhydrazine
Quercetin
SOX7
eng
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
2017-12-01
37
2
245
259
10.21608/besps.2017.28976
28976
Original Article
Study of MiRNA-155 Gene Expression in Egyptian Patients with Gene Expression in Egyptian Patients with Chronic Hepatitis C Viral infection
Wesam elfar
1
Shaden Hanafy
2
Hala El-Said
3
Ashraf El-Fert
4
Department of Molecular Biology*,Genetic Engineering and Biotechnology Research Institute EL-Sadat University
Departments of Clinical Biochemistry National Liver Institute, Menoufia University
Departments of Clinical Biochemistry National Liver Institute, Menoufia University
Department of Molecular Biology*,Genetic Engineering and Biotechnology Research Institute EL-Sadat University
Objective: to evaluate the miRNA -155 expression in patients with chronic HCV infection & correlate it with hepatitis C viral load and treatment response.Background: MicroRNAs (miRNAs), are small, single-stranded, noncoding RNAs that consist of 20 to 25 base pair (bp).It is a class of small RNAs that regulate mRNA translation and function as oncogenes or tumor suppressor genes. In patients infected with HCV, miRNA-155 expression levels were markedly increased and promote hepatocyte proliferation and tumorigenesis by modulating Wnt signaling.Subjects and Methods: This study was conducted on 20 HCC patients, 60chronic liver disease (CLD) patients due to HCV infection subdivided in to20 patients with HCVnaive treatment,20 patients responder treatment to interferon,20 patients non responder treatment to interferon patients and 20 healthy subjects matching age and gender. Serum AFP was measured for all participants. The relative expression of miRNA-155 was determined in whole blood samples using Real-time polymerase chain reaction.Results: The results revealed over expression of miRNA-155 in each of HCC patient group, patients with HCVnaive treatment and patients non responder to interferon treatment. However, miRNA-155 showed down expression inpatients responder to interferon treatment.miRNA-155 expression was positively correlated with presence of cirrhosis, increased number of focal lesions, larger size of tumor, advanced tumor stage and presence of vascular invasion. From ROC curve analysis, the best cutoff of miRNA-155 chosen to differeniate HCC cases from non HCC subjects was 3.41RQs(Fold expression), and at this point the sensitivity, specificity, +ve predictive value (PPV), -ve predictive value (NPV) and Accuracy were 88.8%,91%, 92.4% ,89.5%,91.4% respectively. For AFP the best cutoff was 85.3ng/ml at this cutoff point the Sensitivity, Specificity , PPV, NPV, Accuracy were (76.2 % , 87.3 % ,, 90.2 % , 72.41 %, 81.0%). Furthermore, combined use of serum AFP and circulating miRNA-155for detection of HCC cases, had the advantage over the use of AFP alone as the sensitivity, specificity, PPV, NPP and overall accuracy were increased (89.32 %, 91.9 %, 93.5%, 90.71 % and 92 % respectively).Conclusion: miRNA-155 expression might be correlated with hepatitis C viral load and treatment response. Also, miRNA-155could be a novel diagnostic and prognostic biomarker for detection of HCC in combination with AFP as well as it could serves as a potential therapeutic target for HCV and HCC infection
https://besps.journals.ekb.eg/article_28976_cafbb024ae1acad170319b866fd9d385.pdf
Hcc, HCV, MiRNA-155
expression