ORIGINAL_ARTICLE
Assessment of the Metabolic Mediators: Fatty Acid Synthase, Lactate Dehydrogenase and miR-181a as Potential Diagnostic Markers in Colorectal Cancer Patients
Background: Cancer cells support their growth and invasion by rewiring their metabolism. Therefore our aim was to explore the value of some metabolic mediators such as serum Fatty Acid Synthase (FASN) level and Lactate Dehydrogenase (LDH) activity as well as CEA, CA19-9 and plasma miR-181a levels as diagnostic markers and evaluating their relationship to the clinicopathological features in colorectal cancer (CRC) patients. The association of fasting blood sugar (FBS) and triglyceride (TG) levels as risk factors for colorectal cancer was also assessed. The study was conducted on 70 colorectal cancer patients and 30 controls. Results: CEA, CA19-9, FASN and miR-181a levels were significantly higher in sera of CRC patients compared to controls. miR-181a levels increased significantly according to stage and grade while CA19-9 increased significantly with grade not stage. LDH activity didn’t show significant difference between CRC patients and control except for stage IV. CA19-9 and FASN were significantly increased among males while miR-181a was significantly elevated in female patients. None of the markers showed significant difference according to tumour site. Weak positive and negative correlations were observed between FASN and TG, between LDH and FBS respectively. Moreover, moderate and strong positive correlations were observed between miR-181a and FBS, between CEA and CA19-9 respectively. Conclusion: FASN showed the best diagnostic value with 96% sensitivity and 95% specificity followed by miR-181a with 95% sensitivity and 90% specificity; both were superior to LDH, CEA and CA 19-9. Moreover, miR-181a had the best association with stage and grade compared to all other studied markers.
https://besps.journals.ekb.eg/article_8098_013f8f43f84e5c24d52fbd1e0ea172a8.pdf
2022-01-01
1
12
10.21608/besps.2018.8098
colorectal cancer
Metabolic mediators. Fatty acid Synthase
Lactate dehydrogenase miR-181a
Amani
Youssef
ahmed3eshra_253@hotmail.com
1
Department of Applied Medical Chemistry, Medical Research Institute, Alexandria University.
LEAD_AUTHOR
Mohamed
Ahmed
2
Department of Clinical Pathology, Military Academy of Medicine, Alexandria Armed Forces Hospital.
AUTHOR
Mohamed
Samir
3
Experimental and clinical surgery department, Medical Research Institute, Alexandria University.
AUTHOR
Ibrahim
Mansour
4
Medical Specialist, Faculty of Medicine and Health Sciences, Sanaa university, Yemen
AUTHOR
Mostafa
El Naggar
5
Department of Cancer Management and Research, Medical Research Institute, Alexandria University.
AUTHOR
Tarek
El Sewedy
6
Department of Applied Medical Chemistry, Medical Research Institute, Alexandria University.
AUTHOR
ORIGINAL_ARTICLE
Vitamin D Receptor Gene Polymorphism Taq in Egyptian Women With Polycystic Ovary Syndrome
Background: Polycystic ovary syndrome (PCOS) is one of the commonest endocrine disorder affecting the women in childbearing period. Accumulating evidences from recent studies indicate that vitamin D receptor (VDR)Taq1(Tt) genetic variants may contribute to the pathogenesis of insulin resistance and polycystic ovary syndrome. The Vitamin-D receptor (VDR) regulates vitamin D levels and calcium metabolism in the body and these are known to be associated with insulin resistance and type-2 diabetes in polycystic ovarian syndrome (PCOS). This study aims to investigate the association of VDR polymorphism Taq1(Tt)and serum 25(OH)D level with PCOS. This study was carried out on140 subjects divided into 2 groups: 70 patients with PCOS (group I) and 70 healthy subjects served as controls (group II). All studied subjects were submitted to full history taking, general clinical examination and laboratory investigations for serum levels of fasting blood glucose, total cholesterol (TC),triglycerides (TG), HDL-c,LDL-c, fasting insulin and 25(OH)D. Also genotyping of VDR polymorphism ( Taq1) was analyzed using the polymerase chain reaction–restriction fragment length polymorphism technique (PCR-RFLP). Results showed high significant statistical differences between the two studied groups regarding BMI (P value <0.001),SBP (P value <0.001) ,DBP (P value <0.001), fasting insulin (P value <0.001), fasting blood glucose (P value <0.001) ,insulin resistance (P value <0.001) ,triacylglycerol (P value <0.001), LDL cholesterol (P value <0.001), serum level of 25 (OH) Vit D (P value <0.001) and VIT D R Taq1 genotype distribution (p value<0.001) with increased frequency of the tt andTt genotype in patients with PCOS and increased frequency of TT genotype in controls. Conclusion: Our results indicate that tt genotype and t allele of VDR TaqI polymorphism and serum level of 25OHD might be risk factors for PCOS.
https://besps.journals.ekb.eg/article_8099_481780644e393ae4882335594d2105ab.pdf
2022-01-01
13
26
10.21608/besps.2018.8099
Gene polymorphism
PCOs
Vitamin-D
Vitamin D receptor
Eman
Abd El Gayed
nagarahmed@yahoo.com
1
Medical Biochemistry Department, Faculty of Medicine, Menoufia University Egypt
LEAD_AUTHOR
Alaa
Abd El Gayed
2
Obstetrics and Gynaecology Department, Faculty of Medicine, Menoufia University Egypt
AUTHOR
ORIGINAL_ARTICLE
At Admission Evaluation of the Score for Neonatal Acute Physiology (SNAP II) and Estimation of Serum Procalcitonin Allow Early Diagnosis of Early-Onset Neonatal Sepsis
Abstract
Objectives: The study aimed to evaluate the discriminative ability of, at admission, estimation of serum high-sensitivity C-reactive protein (hsCRP), interleukin-6 (IL-6) and procalcitonin (PCT) between neonates with various grades of early-onset sepsis (EOS) using the Score for Neonatal Acute Physiology (SNAP II). Patients & Methods: The study included 87 neonates with suspected EOS within the 1st week of life. All neonates were evaluated using the SNAP II and scores >40 indicate severe, 20-40 indicate moderate and score <20 indicate mild infection. At admission, venous blood samples were obtained for blood culture, total (TLC) and differential leucocytic count and ELISA estimation of serum hsCRP, IL-6 and PCT.Results: EOS neonates were categorized as Confirmed (n=44), Suspected (n=18) and EOS-free (n=25) according to severity of clinical sepsis and result of blood culture. Estimated laboratory parameters were significantly higher in patients than controls and in EOS than EOS-free neonates. Serum hsCRP and IL-6 levels could not, while PCT could differentiate between neonates with confirmed or suspected EOS. ROC curve analysis defined high serum PCT and IL-6, SNAP II score, neutrophil percentages, serum hsCRP and TLC as significant predictors for positive blood culture in decreasing order of significance, while Regression analysis defined high serum PCT as a persistently significant predictor for positive culture, followed by high serum IL-6 and high SNAP II score. Combined SNAP II scoring and serum PCT could define 61.4%, while combined estimation of serum hsCRP, IL-6 and PCT levels could define 52.3% of neonates with combined EOS. Conclusion: Combined estimation of hsCRP, IL-6 and PCT could increase the diagnostic yield of neonatal sepsis; however, clinical evaluation using SNAP II score and serum PCT did better and could define neonates with positive blood culture earlier so as to allow early treatment.
https://besps.journals.ekb.eg/article_8101_069d0b98c63c516198904a04b1ac02f5.pdf
2022-01-01
27
37
10.21608/besps.2018.8101
Early-onset neonatal sepsis
Score for Neonatal Acute Physiology
Procalcitonin
Interleukin-6
High-sensitivity
C- reactive protein
Yehia
Abdel Maqsoud
1
Department of Pediatrics, Faculty of Medicine, Benha University
AUTHOR
Ahmed
Sobeih
2
Department of Pediatrics, Faculty of Medicine, Benha University
AUTHOR
Effat
Assar
3
Department of Pediatrics, Faculty of Medicine, Benha University
AUTHOR
Deena
Elshabrawy
4
Department of Clinical Pathology, Faculty of Medicine, Benha University
AUTHOR
Manal
Hassan
5
Department of Medical Biochemistry, Faculty of Applied Medical Sciences, 6 October University
LEAD_AUTHOR
Raafat
Mohammed
6
Fellow and Researcher in Biochemistry, Clinical Pathology Department, Benha University, Benha, Egypt
AUTHOR
ORIGINAL_ARTICLE
Role of Placental Mitochondria in Development of Pre-eclampsia: Focus on Mitochondrial Dynamics, Redox Signaling and Apoptosis
Background: Mitochondrial dysfunction has been incriminated in the pathogenesis of pre-eclampsia (PE). This study aimed at evaluating the contribution of mitochondrial dynamics, biogenesis, redox signaling and apoptosis in the pathogenesis of mitochondrial dysfunction in pre-eclamptic placenta. Forty pregnant females were classified equally into two groups: Group І (control group), included normotensive pregnant females and Group ІІ (PE group) included, pre-eclamptic pregnant females. After delivery, placental tissue samples were collected for estimation of mRNA expression levels of Mitofusin2 (Mfn2) using quantitative real-time PCR . Dynamin related protein 1 (Drp1), mitochondrial Cytochrome c release ,and 3-nitrotyrosine (3-NT) were measured by ELISA . Mitochondrial complex I, and citrate synthase enzyme activity were assessed spectrophotometrically. ATP levels, Caspase-9 activity , inorganic nitrites and nitrate levels ,and superoxide dismutase (SOD) activity were measured by colorimetric assay kit. Pre-eclamptic placentae showed significant decrease in complex I, ATP levels and citrate synthase activity. mRNA expression of Mfn2 were downregulated with marked elevation of DRP1 protein levels .There were altered redox status as judged by the elevation of NO and protein nitration with reduction in the total SOD activity in pre-eclamptic placentae. There were also activation of the mitochondrial pathway of apoptosis as judged by release of cytochrome c from the mitochondrial intermembrane space into the cytosol and significant increase in caspase-9 levels in Pre-eclamptic placentae compared to controls. Our data strongly nominated significant association between mitochondrial dysfunction, disturbed dynamics, altered redox status and the susceptibility to apoptosis in pre-eclamptic placenta as key players in the multifactorial pathogenic mechanisms of PE
https://besps.journals.ekb.eg/article_8103_040b903c5c7956acb03c462a934ce3c6.pdf
2022-01-01
38
52
10.21608/besps.2018.8103
Pre-eclampsia
Mitochondrial dynamics
Mitofusins 2 (Mfn2)
Dynamin-related protein 1 (DRP1)
Nitrosative stress
Soha
Zakaria
drsohasaid@yahoo.com
1
Departments of Medical Biochemistry, Faculty of Medicine, Tanta University
LEAD_AUTHOR
Hanaa
Gaballah
2
Department of Medical Biochemistry, Faculty of Medicine, Tanta University
AUTHOR
Ahmed
Hagras
3
Department of Obstetrics and Gynecology, Faculty of Medicine, Tanta University
AUTHOR
ORIGINAL_ARTICLE
Effect of Chronic Exercise on Irisin Plasma Level and Browning of White Adipose Tissue in Rat Models with Obesity or Type 2 Diabetes Mellitus
Objective: To study the effect of swimming exercise training on plasma irisin level and browning of white adipose tissue in rats models with obesity or type 2 diabetes mellitus (T2DM). Methods: 60 male rats were divided into 4 groups, I) Control group, II) Exercise trained group, III) Obese group: includes 2 subgroups; subgroup A received high-fat diet and subgroup B received high-fat diet associated with daily exercise training and IV) Type 2 diabetic group: includes 2 subgroups; subgroup A and B. Both subgroups have T2DM. Subgroup B, in addition, was associated with daily exercise training after induction of T2DM. Plasma samples were analyzed for irisin, fasting insulin, fasting blood glucose and lipid profile.Levels of uncoupling protein-1 in subcutaneous abdominal adipose tissue homogenates (UCP-1 SAT) and uncoupling protein-1 in perinephric adipose tissue homogenates (UCP-1 PAT) were measured.Results: Subgroup IIIA had significantly higher irisin levels and significantly lower levels of UCP-1 SAT and UCP-1 PAT compared with group I. Subgroup IVA had significantly lower levels of irisin, UCP-1 SAT and UCP-1 PAT compared to controls. Chronic exercise significantly increased irisin, UCP-1 SAT, and UCP-1 PAT levels in group II, subgroup IIIB and subgroup IVB. Conclusion: Swimming exercise increases the plasma irisin level in normal, obese and type 2 diabetic rats inducing browning of white adipose tissue. Irisin is increased in obesity which may be attributed to a state of irisin resistance. T2DM is associated with lower plasma irisin levels
https://besps.journals.ekb.eg/article_8106_a50b6c81c09dc33672a2d1d5974da1fa.pdf
2022-01-01
53
65
10.21608/besps.2018.8106
Irisin
Browning
Obesity
Type 2 diabetes mellitus
Chronic exercise
Zeinab
EL-Said
zeinabelsaid1@gmail.com
1
Department of Physiology, Faculty of Medicine, Mansoura University, Egypt
LEAD_AUTHOR
Gehan
El-Wakeel
2
Department of Physiology, Faculty of Medicine, Mansoura University, Egypt.
AUTHOR
Amr
Abbas
3
Department of Physiology, Faculty of Medicine, Mansoura University, Egypt.
AUTHOR
Omayma
Saleh
4
Department of Internal Medicine, Faculty of Medicine, Mansoura University, Egypt.
AUTHOR
Mohamed
Sarhan
5
Department of Physiology, Faculty of Medicine, Mansoura University, Egypt.
AUTHOR
ORIGINAL_ARTICLE
Invasive and Non-Invasive Evaluation of antifibrotic effect of Losartan on Experimentally Induced Liver Fibrosis: A Biochemical and Histological Study
Background: Liver fibrosis and cirrhosis are major causes of morbidity and mortality worldwide. Precise determination of liver fibrosis stage is crucial for the choice of optimal therapies. Liver biopsy is the most accurate method used to evaluate progression of chronic liver diseases but it has several complications. Recently many non-invasive markers (NIMs) for assessing liver fibrosis have been developed. Aim: This study was done to evaluate invasive method and non-invasive methods in assessment of CCl4 induced liver fibrosis and the reversibility of liver fibrosis by angiotensin receptor blocker (Losartan). Methods: Forty-two adult albino rats were divided intoControl group (n =12), CCl4 treated groub (n = 18) and Losartan treated group (n=12). At the assigned times, serum SGPT and SGOT were assayed by thecolorimetric method and serum tissue inhibitor metalloproteinase 1 (Timp1) by Elisa. Livers of rats were stained with H & E for histopathological examination, Masson trichrome and Sirius red for liver fibrosis, and Immunostaining for Timp1. Results & Conclusions: The results concluded that non-invasive methods can express the difference that occur in liver fibrosis but it cannot estimate its degree so it cannot replace the liver biopsy. Further research could identify other promising non-invasive methods.
https://besps.journals.ekb.eg/article_8110_050dae85eb8f7a0ec41ef98f2fd13be8.pdf
2022-01-01
66
76
10.21608/besps.2018.8110
Liver fibrosis
Losartan
Serum TIMP1
histopathology
Heba
El-hessy
1
Department of Anatomy and Embryology, Faculty of Medicine, Mansoura University, Egypt
AUTHOR
Noha
Hazem
nohadata@gmail.com
2
Medical Biochemistry department, Mansoura University, Egypt
AUTHOR
Dalia
Saleh
3
Department of Anatomy and Embryology, Faculty of Medicine, Mansoura University, Egypt.
AUTHOR
Kamal
Botros
4
Departments of Anatomy and Embryology, Faculty of Medicine, Mansoura University, Egypt.
AUTHOR
ORIGINAL_ARTICLE
Plasma Circular RNA (0054633) Expression as a Biomarker for Prediabetes and Type 2 diabetes mellitus
Background: Circular RNAs are a type of closed non-coding RNAs, lack the terminating 5'-capand 3'-polyadenylated tail structures that present in linear RNAs. They have higher biologicalstability because of their resistance to RNA exonucleases and in some tissues; their expressionlevels are ten times higher than other types of RNA. CircRNAs act as biomarkers for variousdiseases including diabetes mellitus as they have been found to affect insulin secretion and β-cellrenewal. Objective: to investigate the role of circRNA (0054633) as a biomarker for pre-diabetesand T2DM. Subjects & Methods: A total number of 149 subjects, selected from endocrinologyunit of Internal Medicine Department, and Munshaat Sultan Family Medicine Clinic Menoufiauniversity hospital, classified into three groups : group I were 55 T2DM patients, group II were 44pre-diabetics and group III including 50 healthy subjects. Expression profile of circRNA(0054633) in plasma of the studied subjects was analyzed by quantitative real- time polymerasechain reaction (Q-PCR). Results: CircRNA (0054633) expression was significantly increasedgradually from controls to the pre-diabetes group to the T2DM group (p<0.05).The sensitivity ofCircRNA (0054633) expression for prediction of diabetes and prediabetes at cutoff point (2.95,1.95) was (92.7%, 77.3%) respectivily and the specificity was (98% and 98.6%) respectivily.The most significant pedictors for pre-diabetes were CircRNA (0054633) expression and BMI,OR (59.8, 24.9 respectivily). Conclusion: Plasma CircRNA (0054633) expression could beconsidered as a predictive and diagnostic biomarker for pre-diabetes and T2DM.
https://besps.journals.ekb.eg/article_8113_3ea9d8c9e51f4a4e8198e16ab994f2d5.pdf
2022-01-01
77
88
10.21608/besps.2018.8113
Circular RNA
Pre-diabetes
T2DM
Sally
El-Hefnway
doctor_sally@rocketmail.com
1
Medical Biochemistry Department, Faculty of Medicine, Menoufia University, Egypt
LEAD_AUTHOR
Nevein
Al-sheikh
2
Medical Biochemistry Department, Faculty of Medicine, Menoufia University, Egypt
AUTHOR
Heba
Kasem
3
Internal Medicine Department, Faculty of Medicine, Menoufia University, Egypt.
AUTHOR
Fatma
El-Esrigy
4
Family Medicine Department, Faculty of Medicine, Menoufia University, Egypt.
AUTHOR
ORIGINAL_ARTICLE
Effects of Cadmium on Kidney Functions and Oxidative Stress In Albino Rats
Background: Cadmium (Cd) is widespread used in industry and extensively disseminated in the environment. As a result of the extensive use of cadmium (Cd) in industry and its extensive dissemination in the environment, numerous studies have focused on the identification of the early stages of Cd-induced renal injury in exposed persons. Following long-term low-level exposure, nearly one-third of the cadmium in the body will be found in the renal tissue. So, the aim of the present work is to evaluate the disturbed renal functions induced by cadmium exposure and its underlying mechanism. Materials and Methods:Twenty male rats were divided into two main groups, ten rats per each group. Group 1 (n=10) was the Cd group in which rats were treated for 13 days with a daily dose of 1 mg CdCl2/kg body weight in sterile 0.9 % NaCl solution subcutaneously. Group2 (n=10) was the vehicle control, they received equivalent volumes of sterile 0.9 % NaCl solution by subcutaneous injections.Results:cadmium administration led to a decrease in serum sodium and potassium level and increase their level in urine. It led to a significant decrease in creatinine clearance and a significant increase in the oxidative stress biomarker tissue malondialdehyde (MDA), as well as, and a significant decrease in serum total antioxidant capacity. Moreover, cadmium led to a significant decrease in glucose uptake by kidney (mg/gm wet tissue/h.) Conclusion: Cd has detrimental effects on kidney functions and renal handling of glucose and this effect is referred to its oxidative stress effect.
https://besps.journals.ekb.eg/article_8114_ad72f4a2aec0c407a2c28362e3068bd7.pdf
2022-01-01
89
99
10.21608/besps.2018.8114
cadmium
kidney
Oxidative Stress
Rats
Mohamed
Adel
madel7744@yahoo.com
1
Department of Medical Physiology, Faculty of Medicine, Mansoura University, Egypt.
LEAD_AUTHOR
Mohamed
Ghalwash
2
Department of Medical Physiology, Faculty of Medicine, Mansoura University, Egypt.
AUTHOR