Role of melatonin on immobilization induced effects in rat soleus muscle

Document Type : Original Article

Authors

Medical Physiology Department, Mansoura Faculty of Medicine, Egypt

Abstract

Objectives: Immobilization results in dramatic losses of skeletal muscle mass. Several reports have strongly implicated oxidative stress as partially causative of disuse atrophy. So, the aim of this study was to investigate the deleterious effects of immobilization upon rat skeletal muscle and to detect the possible protective role of melatonin on these effects. Methods: male Wister albino rats were subjected to immobilization procedure, then divided into (C) control group, and three treated groups with melatonin either once daily at morning (M1) group or at night (M2) group or twice daily (M3) group. Soleus muscle was used to detect myosin heavy chain (MHC) distribution, gene expression and histological examination. Results: The study demonstrated that treatment with melatonin once at night and twice per day prevented the deleterious effects of immobilization by increasing the total antioxidant capacity, decreasing the muscle atrophy F-box (MAFbx) and muscle-specific RING finger-1 (MuRF-1) and increasing in insulin-like growth factor 1 receptors (IGF-1R) gene expression. Conclusions: The results of this study provide evidence that melatonin administration reduces the deleterious effects of immobilization on skeletal muscle. SO, exogenous melatonin may be a possible candidate for hormonal therapy in immobilization-induced muscle atrophy.

Main Subjects

Files

Share

How to cite

Statistics