Role of Hemeoxygenase-1 on Ischemia Induced Preeclampsia-Like Syndrome in Rats

Document Type : Original Article

Authors

1 Departments of Medical Physiology, Faculty of Medicine, Tanta University, El-Gharbia, Egypt

2 Department of MedicalBiochemistry, Faculty of Medicine, Tanta University, El-Gharbia, Egypt

3 Departments of Molecular Biology and Pathology, Faculty of Medicine, Tanta University, El-Gharbia, Egypt

Abstract

 



Background: Placental hypoxia/ischemia has been implicated as a central corn in the development of preeclampsia. One particularly useful model to study the impact of placental ischemia is the reduced uterine perfusion pressure model as it induces preeclampsia - like syndrome. Aim: This study aimed to demonstrate the effect of hemeoxygenase-1 on placental hypoperfusion in reduced uterine perfusion pressure model. Methods: The study was carried out on 50 timed pregnant female wistar rats divided into 5 equal groups; normal pregnant, reduced uterine perfusion pressure, reduced uterine perfusion pressure + Cobalt (III) Protoporphyrin IX Chloride, reduced uterine perfusion pressure + Cobalt (III) Protoporphyrin IX Chloride + tin-protoporphyrin IX and reduced uterine perfusion pressure + tin-protoporphyrin IX. In all these groups, number and weight of pups and maternal mean arterial blood pressure were measured. Then, blood, urine and placental samples were collected for measurement of microalbumin creatinine ratio, soluble fms-like tyrosine kinase, vascular endothelial growth factor, tumor necrosis factor- α, super oxide dismutase and lactic dehydrogenase. Histopatholgoical examination of placentas of all groups was also done. Results and Conclusion: Reduced uterine perfusion pressure produced preeclampsia -like syndrome (hypertention, proteinuria and endothelial dysfunction). All biochemical parameters and histopathological pictures have been significantly improved by hemeoxygenase-1induction. So, hemeoxygenase-1 can be considered as a potential therapeutic target in the treatment of preeclampsia in the near future.

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