@article { author = {Rabei, Mohammed and Abd-Elmoniem, Hanaa and Sarhan, Mohammed and Abo-Elmaaty, Nisreen and Ali, Lashin}, title = {Possible Protective Role of Erythropoietin in Diabetic Cardiomyopathy in Rats}, journal = {Bulletin of Egyptian Society for Physiological Sciences}, volume = {37}, number = {2}, pages = {92-109}, year = {2017}, publisher = {Egyptian Society for Physiological Sciences}, issn = {1110-0842}, eissn = {2356-9514}, doi = {10.21608/besps.2017.8231}, abstract = {There are controversial experimental works about the way of EPO in protection againstDCM; whether EPO action is through making better metabolic condition and glycemiccontrol or the antioxidant and antiapoptotic effect. So ,the aim of this study was to evaluatethe modulating effects of EPO on some biochemical markers in diabetic cardiomyopathy.Fifty male Sprague Dawley rats were divided into five groups of 10rats each. The rats ofgroup Ⅰ served as normal control. Group Ⅱ received HFD for 1month and STZ in aconcentration of 35mg/kg intraperitoneal. Group Ⅲ:(Diab + Gliben) diabetic rats treatedwith glibenclamide, Gliben was given at a dose (0.6 mg/kg orally) for 4 weeks, Group Ⅳ:Diabetic rats treated with EPO; twice a week 4 weeks' duration. (1,000 IU/kg) and Group Ⅴ:diabetic rats treated by both (EPO + Gliben) with the same previous doses for 4 weeks'duration. At the end of experiment serum samples and cardiac tissues were obtained from thesacrificed rats. Cardiac enzymes, blood glucose levels, serum insulin level and oxidativestress markers were estimated. Diabetic cardiomyopathy changes were confirmed byelectrophysiological and histopathological results as well as increased serum activity o fcardiac enzymes and high blood glucose levels. EPO exhibited myocardial protection in ratswith DCM, at least in part through antioxidant effect and better glycemic control. However,this study was based on animal models in vivo, and detailed molecular mechanisms should befurther investigated using in vitro studies. Clinical trials may also be considered to validatethe results in humans, following further pre-clinical studies.}, keywords = {Erythropoietin (EPO),Diabetic cardiomyopathy,Glibenclamide,Oxidative Stress,High Fat Diet (HFD)}, url = {https://besps.journals.ekb.eg/article_8231.html}, eprint = {https://besps.journals.ekb.eg/article_8231_f08c4fcfe405841692c9ef7dd878162d.pdf} } @article { author = {Azab, Naglaa and Tawfik, Said}, title = {Potential Molecular Biomarkers for Prediction of Coronary Artery Disease, its Severity and Extent}, journal = {Bulletin of Egyptian Society for Physiological Sciences}, volume = {37}, number = {2}, pages = {110-128}, year = {2017}, publisher = {Egyptian Society for Physiological Sciences}, issn = {1110-0842}, eissn = {2356-9514}, doi = {10.21608/besps.2017.8232}, abstract = {Coronary artery disease (CAD) has high morbidity and mortality, therefore accurate detection of CAD and atherosclerotic plaque burden is essential. Invasive coronary angiography (ICA) can confirm the presence of luminal stenosis caused by atherosclerotic plaque and is an important tool for the quantification of CAD plaque burden. However, it has many limitations. Therefore, new methods for accurate noninvasive diagnosis of CAD using genetic markers were exerted. This study aimed at evaluation of new genetic biomarkers in diagnosing CAD and determining its severity and extent, using Gensini score as a scoring model. Sixty two subjects who had undergone coronary angiography and analyzed for the presence, severity and extent of CAD using Gensini score were selected for this study. Twenty subjects had normal Gensini score, 24 subjects were with mild to moderate CAD and 18 subjects were with severe CAD. Extraction of total RNA from whole blood was done together with PCR amplification of PNPLA2, TUBA3, FTL and AHR genes and the house keeping gene GAPDH. It was found that PNPLA2 and TUBA3 were significantly lower in CAD patients and mild/moderate CAD than in controls. However, PNPLA2 was significantly higher in severe CAD than in mild/moderate CAD. Moreover, PNPLA2 gene had The highest specificity for CAD prediction of (100%), followed by TUBA3 (60%) then FTL and AHR (each 40%), and both PNPLA2 and FTL gene had the highest sensitivity for CAD diagnosis (80.95%), followed by TUBA 3 (76.19%), then AHR (71.43%). On the other hand, PNPLA2 showed the highest specificity in CAD severity prediction (83.33%), followed by TUBA3, FTL and AHR with equal specificity (each 50%), and TUBA 3 showed the highest sensitivity (66.67%) followed by PNPLA2,, FTL and AHR with equal sensitivity (each 55.56%). In conclusion, these genes are useful in prediction of CAD and CAD severity especially emphasizing PNPLA2 and TUBA3 and further studies on larger population number and other genes are recommended.}, keywords = {molecular Biomarkers,Coronary artery disease (CAD),plaque burden}, url = {https://besps.journals.ekb.eg/article_8232.html}, eprint = {https://besps.journals.ekb.eg/article_8232_ffe256f25ae9641484fefccc0f55a8c0.pdf} } @article { author = {Al-kholy, Adel and Salem, Ahmed and Hagras, Maha}, title = {Combined Estimation of Serum Procalcitonin Levels and Clinical Pulmonary Infection Score Improves Predictability for Survival of Ventilation-associated Pneumonia Patients}, journal = {Bulletin of Egyptian Society for Physiological Sciences}, volume = {37}, number = {2}, pages = {129-142}, year = {2017}, publisher = {Egyptian Society for Physiological Sciences}, issn = {1110-0842}, eissn = {2356-9514}, doi = {10.21608/besps.2017.8243}, abstract = {Objectives: To evaluate predictability of estimation of C-reactive protein (CRP) and procalcitonin (PCT) levels for diagnosis and survival of ventilator-associated pneumonia (VAP) patients. Patients & Methods: The study included 53 VAP patients and 37 No VAP patients who were assessed using Clinical Pulmonary Infection Score (CPIS) and Acute Physiology and Chronic Health Evaluation II (APACHE II) scores. Blood samples were collected on D0 and D4 for estimation of serum CRP and PCT levels. Patients were managed according to Surviving Sepsis Campaign guidelines. The 28-day mortality rate (MR) and the predictors for mortality were determined. Results: Total MR was 43.3% with significant difference between both groups. APACHE II and CPIS scores were significantly higher in non-survivors of both groups and in VAP than No VAP patients. Serum CRP and PCT levels were significantly higher in VAP patients and in non-survivors than survivors. Change of CRP level was significantly higher in No VAP than VAP survivors, while change of PCT levels was significantly higher in VAP survivors than non-survivors. High CPIS score and D0 PCT level, but low decreases of CRP and PCT levels are positive predictors for VAP diagnosis. High D4 PCT level and CPIS scores, but low decreases of PCT levels are significant predictors for mortality. Conclusion: VAP had high 28-day mortality rate. Combined evaluation of CPIS score and PCT levels improved the ability to diagnose VAP and low levels of both are independent predictors of survival.}, keywords = {Ventilator-Associated Pneumonia,C-reactive protein,Procacitonin,CPIS score,survival rate}, url = {https://besps.journals.ekb.eg/article_8243.html}, eprint = {https://besps.journals.ekb.eg/article_8243_937ce2f7a2b137774c5ff3c10bb2cf4b.pdf} } @article { author = {Monir, Rehan and Badawy, Abdelnaser and Mokhtar, Naglaa and Nabil, Hanan and Alruwaili, Mohammed}, title = {ABCA1 gene polymorphism and oxidative stress in polycystic ovary syndrome: Role of antioxidant therapy for prevention and control}, journal = {Bulletin of Egyptian Society for Physiological Sciences}, volume = {37}, number = {2}, pages = {143-153}, year = {2017}, publisher = {Egyptian Society for Physiological Sciences}, issn = {1110-0842}, eissn = {2356-9514}, doi = {10.21608/besps.2017.8244}, abstract = {Polycystic ovary syndrome (PCOS) is a common health problem that affects women in childbearing age. Recent studies discussed the relation of oxidative stress with PCOS and suggested that the antioxidants may improve the prognosis of PCOS. Changes happened in ATP binding cassette transporter 1 (ABCA1) gene encoding a protein regulating entry and exit from cell membrane may contribute to dyslipidemia in patients with PCOS. The role of oxidative stress and ABCA1 gene polymorphism in the pathogenesis of PCOS was evaluated along with the effect of antioxidants in the prevention and treatment of this disease. The present study included 90 female patients with PCOS and 90 healthy women represented the control group. Patients with PCOS were further divided into two subgroups. The patients included in subgroup 1a were treated with antioxidant and hormonal therapy while patients included in subgroup 1b were treated with hormonal therapy only. ABCA1gene polymorphism and oxidative stress parameters were investigated in all groups. The level of the oxidant marker; MDA was higher in PCOS patients when compared to controls. In addition, the levels of GSH, vitamin E, ascorbic acid, and selenium were significantly decreased, while SOD and GPx activities were increased in PCOS patients when compared to controls. The response to treatment among PCOS patients was better in group 1a than group 1b. The frequency of ABCA1 AA genotype was higher in PCOS patients than the control group (32.2% and 11.1% respectively). It was concluded that oxidative stress may lead to PCOS which raises the possibility of using antioxidants for the prevention and control of PCOS. In addition, the polymorphism in ABCA1 gene may be associated with genetic susceptibility for PCOS.}, keywords = {PCOs,Oxidative Stress,ABCA1 gene polymorphism}, url = {https://besps.journals.ekb.eg/article_8244.html}, eprint = {https://besps.journals.ekb.eg/article_8244_8f9139c50ba1a07798a40551c99e9891.pdf} } @article { author = {Bakr, Ramadan and El-Mahallawy, Ibrahim and Agha, Mohammed and El-Ghobashy, Yasser and Said, Mai}, title = {Assessment of Serum Level of Ghrelin in Patients with Chronic Obstructive Pulmonary Disease}, journal = {Bulletin of Egyptian Society for Physiological Sciences}, volume = {37}, number = {2}, pages = {154-164}, year = {2017}, publisher = {Egyptian Society for Physiological Sciences}, issn = {1110-0842}, eissn = {2356-9514}, doi = {10.21608/besps.2017.8252}, abstract = {Objectives: to study the relationship between serum level of ghrelin and cachexia in patients with COPD. Background: Chronic obstructive pulmonary disease is a major cause of chronic morbidity and mortality throughout the world. COPD involves several systemic features. The link between ghrelin, cachexia and COPD needs to be clarified.Materials and methods: Serum levels of ghrelin were measured in 60 COPD patients (divided into 3 groups according to their BMI) and 30 control subjects using human ghrelin ELISA kits. Results: There was a significant increase in ghrelin level in COPD patients compared to control subjects. There was significant increase in serum ghrelin level in underweight COPD subgroup compared to normal weight and overweight subgroups. Serum levels of ghrelin in COPD patients were affected by the level of pulmonary obstruction. Conclusions: Serum level of ghrelin is increased in COPD patients, and is positively correlated with BMI and disease severity.}, keywords = {ghrelin,COPD,Cachexia}, url = {https://besps.journals.ekb.eg/article_8252.html}, eprint = {https://besps.journals.ekb.eg/article_8252_7161ba6e3dac1b6047aca71b2f9b93f7.pdf} } @article { author = {Abdel-Mohsen, Mohamed and Youssef, Amany and El Sewedy, Tarek and Abou-Youssef, Morsy and Maghraby, Hala and El-Sarha, Ashgan and Abd-Elrazak, Mohammad and El-Gamal, Ahmed and Dardeer, Amr}, title = {Protective Effect of Copper (I)-Nicotinate Complex on DAB-Induced Hepatocellular Carcinoma in Rats}, journal = {Bulletin of Egyptian Society for Physiological Sciences}, volume = {37}, number = {2}, pages = {165-175}, year = {2017}, publisher = {Egyptian Society for Physiological Sciences}, issn = {1110-0842}, eissn = {2356-9514}, doi = {10.21608/besps.2017.8258}, abstract = {The aim of the present work was to evaluate the effect of copper (I)-nicotinate complex (CNC) on experimentally 4-Dimethylaminoazobenzene (DAB)-induced hepatocellular carcinoma (HCC) in male rats. In addition to the histopathological examination we measured hepatic glutathione content, malondialdehyde, nitric oxide levels, lamin B1 m-RNA, caspase-3 activity and serum interleukin-12 level after 2, 4, 6, 8 months from commencement of the experiment. Results: histopathological examination showed HCC development after 4 months of DAB administration. The hepatic glutathione content, lamin B1 m-RNA and nitric oxide levels were significantly elevated, while malondialdehyde, interlukin-12 levels and caspase-3 activity were significantly decreased with the progression and development of the HCC. On the other hand, administration of CNC one month before DAB delayed the development of neoplastic growth to the 8th month. Interestingly, when CNC was administered one month after DAB, it successfully prevented HCC development throughout the whole experiment as confirmed by histopathological data and explained by biochemical markers, as glutathione, lamin B-1 and nitric oxide were significantly declined but malondialdehyde, interlukin-12 and caspase-3 activity were significantly elevated compared to that in corresponding control group. Conclusion: CNC was able to delay or prevent HCC development in rats fed with the potent liver carcinogen DAB. Our data shows that CNC exerts its anti-tumour effects through modulating oxidative stress status as well as the machinery of apoptosis and angiogenesis. Therefore, CNC may be used as a potential protective anticancer agent.}, keywords = {Copper (I)-Nicotinate Complex,Hepatocellular carcinoma,Angiogenesis,apoptosis,Anticancer}, url = {https://besps.journals.ekb.eg/article_8258.html}, eprint = {https://besps.journals.ekb.eg/article_8258_e1fcde8f9909bc56e4c5abc7aabdc98f.pdf} } @article { author = {Abdu Allah, Azza and Abd El Gayed1, Eman and Abd El Gayed, Alaa and Desouky, Maha}, title = {Gestational Diabetes Mellitus And Leptin Gene polymorphism In Egyptian Women}, journal = {Bulletin of Egyptian Society for Physiological Sciences}, volume = {37}, number = {2}, pages = {176-188}, year = {2017}, publisher = {Egyptian Society for Physiological Sciences}, issn = {1110-0842}, eissn = {2356-9514}, doi = {10.21608/besps.2017.8263}, abstract = {Gestational diabetes is blood sugar elevation during pregnancy and exposes women to the risk for development of type 2 diabetes mellitus in later years of life. The LEP rs7799039 (2548G/A) polymorphism in the 5´ region of the LEP gene was reported not only to be associated with overweight but also to have a strong influence on leptin gene expression and adipose tissue secretion. The aim of this study is to evaluate the distribution of LEP rs7799039 (2548G/A) polymorphism and its plasma level in patients with gestational Diabetes Mellitus. This study was carried out on160 subjects divided into 2 groups; 80 patients with GDM (group I) and 80 healthy subjects served as controls (group II). All studied subjects were submitted to full history taking, general clinical examination and laboratory investigations including fasting and 2 hour post prandial blood glucose, total cholesterol (TC),triglycerides (TG), HDLc, LDLc, glycated hemoglobin (HbA1c), plasma leptin, fasting serum insulin, HOMA- IR and Genotyping of LEP rs 7799039 (2548G/A) polymorphism was analyzed by the TaqMan allelic Discrimination Assay Technique. Results showed significant statistical differences between the two studied groups regarding family history and BMI. Significant statistical difference between group (I) and group (II) regarding plasma leptin level (P value <0.001), serum insulin (P value <0.001), insulin resistance (P value <0.001) and LEP rs 7799039 (2548G/A) genotype distribution with increased frequency of the AG and AA genotype in patients with gestational diabetes (P value <0.001) and increased frequency of GG genotype in controls. Conclusion: This results indicate that leptin plays a role in increasing insulin sensitivity, LEP rs7799039 (2548G/A) and its plasma level can contribute to susceptibility to GDM and might be used for screening of the early detection of GDM.}, keywords = {Gestational diabetes,leptin,Gene polymorphism}, url = {https://besps.journals.ekb.eg/article_8263.html}, eprint = {https://besps.journals.ekb.eg/article_8263_768be084f4a1cdb3282f9e4b88d6b41b.pdf} } @article { author = {Al-Sherbeny, Mohamad and Hassaan, Manal}, title = {Disturbed Inflammatory/Anti-inflammatory Milieu and Activation of Primary Phase Reactants may Underlie Development and Recurrence of First trimester Pregnancy Loss.}, journal = {Bulletin of Egyptian Society for Physiological Sciences}, volume = {37}, number = {2}, pages = {189-198}, year = {2017}, publisher = {Egyptian Society for Physiological Sciences}, issn = {1110-0842}, eissn = {2356-9514}, doi = {10.21608/besps.2017.8264}, abstract = {Objectives: Estimation of serum levels of tumor necrosis factor-α (TNF-α), interleukin (IL-) 6 and 10 and alpha-1 antitrypsin (AAT) in pregnant women with current pregnancy loss (CPL) and non-pregnant women with history of recurrent PL (RPL) compared versus women with normal pregnancy (NP). Patients & Methods: All studied women underwent clinical and vaginal ultrasonographic examination for assurance of inclusion criteria and then gave blood samples for ELISA estimation of studied parameters. Results: Patients of CPL and RPL groups showed significantly high serum TNF-α and IL-6 levels with significantly lower serum IL-10 levels than women of NP group with significant difference between CPL and RPL groups. Serum AAT levels were significantly lower in CPL group than RPL and NP groups with significantly lower levels in RPL than NP group. High serum TNF-α and low AAT levels showed high positive predictive value (PPV) and sensitivity, respectively, for possibility of presence or recurrence of PL. Conclusion: Disturbed serum levels of pro- and anti-inflammatory cytokines could diagnose CPL with high PPV and persistence of their disturbed levels may predispose to RPL. Activation of primary phase reactant system may underlie, accompany or initiate PL, so low serum AAT during 1st trimester and its persistently low serum levels during inter-pregnancy intervals could be used as screening test for presence or liability for development of PL.}, keywords = {First trimester pregnancy loss,Inflammatory cytokines,Alpha-1 antitrypsin}, url = {https://besps.journals.ekb.eg/article_8264.html}, eprint = {https://besps.journals.ekb.eg/article_8264_b1bdaf0f58db8030d5a9d78a9f0d46b5.pdf} } @article { author = {Ghoniemy, Helmy and Mohamed, Shereen and El Badya, Hoda and Abou- Zied, Mohamed and Sayed Elsayh, Hebat Allah and Elfiky, Abir}, title = {Cytokine assessment and Immunomodulatory Effect of Bee Venom in HBV&HCV Infected Patients}, journal = {Bulletin of Egyptian Society for Physiological Sciences}, volume = {37}, number = {2}, pages = {199-207}, year = {2017}, publisher = {Egyptian Society for Physiological Sciences}, issn = {1110-0842}, eissn = {2356-9514}, doi = {10.21608/besps.2017.8265}, abstract = {Background: Hepatitis B &Hepatitis C viral infections are common health problems worldwide. New strategies for treatment are now evolving. The therapeutic application of bee venom has been used in traditional medicine to treat many diseases. Aim: The aim of this study was to investigate the effect of bee-sting (venom) therapy on progression of chronic viral hepatitis B & C and on the levels of proinflammatory cytokines IL-1β , IL -2 and IL -6 and the anti inflammatory IL -10 in HBV&HCV infected patients. Methods: 67 Egyptian patients with chronic hepatitis were enrolled, 20 of them have HBV and 47 have HCV. Bee stings were administered using live bees at apipunctur point. CBC, liver enzymes, Hepatitis B &C RT-PCR, IL-1β, IL -2, IL -6 and IL -10 were estimated before & after bee stings therapy. Results: there was a significant decrease in ALT; AST (P≤0.05) after bee venom injection in both types of hepatitis. viral load was decreased during the course of treatment, it became negative after 9 months therapy in all cases of HBV infection and in 34.04% of HCV infection. IL-1β, IL -2, IL -6 and IL -10 levels were aalso significantly decreased after 9 months therapy in both types of hepatitis patients. Conclusions: bee venom stings decreased the viral load and IL-1β, IL -2, IL -6 and IL -10 after 9 month's therapy in both types of hepatitis.}, keywords = {Bee venom,Hepatitis B virus,Hepatitis C virus,Cytokines,Bee venom therapy}, url = {https://besps.journals.ekb.eg/article_8265.html}, eprint = {https://besps.journals.ekb.eg/article_8265_d0d65d66d25584357b9a1f853c80c0a4.pdf} } @article { author = {Zakaria, Soha and ElBatsh, Maha and Mowafy, Shorouk}, title = {Effects of Curcumin on Autophagy and Nrf2 signaling pathway in a Rat Model of High fructose diet induced Steatohepatitis}, journal = {Bulletin of Egyptian Society for Physiological Sciences}, volume = {37}, number = {2}, pages = {208-226}, year = {2017}, publisher = {Egyptian Society for Physiological Sciences}, issn = {1110-0842}, eissn = {2356-9514}, doi = {10.21608/besps.2017.8268}, abstract = {Increased fructose consumption predisposes to nonalcoholic steatohepatitis (NASH).There no effective treatment to be used as the second line when lifestyle modification is insufficient .So, the aim of this study was to evaluate the effect of Curcumin on some biochemical markers in fructose induced NASH . Sixty male albino rats were divided randomly and equally into three groups. Group I (control group). Group II (NASH group), received 70% fructose for 5 weeks .Group III (curcumin treated NASH group), fed as group II followed by daily administration of curcumin at a dose of 50 mg/kg orally for 6 weeks. Blood samples were collected to measure lipid profile, and liver enzymes activities . Liver tissues samples were collected for estimation of mRNA expression levels of nuclear factor E2-related factor-2 (Nrf2) using quantitative real-time PCR ,levels of Beclin 1, AMPK, IL6 and Nrf2 DNA-binding activity by ELISA method, and oxidative stress markers malondialdehyde (MDA), superoxide dismutase (SOD) ,GSH spectrophotometrically. NASH was confirmed by histopathology as well as increased activity of liver enzymes and marked dyslipidemia .Curcumin ameliorated biochemical disturbance in NASH by upregulating autophagy process through increasing Beclin 1, increasing AMPK and hampered IL6 levels in the liver . It also restored redox balance as evident by suppressing MDA formation and preservation of intracellular antioxidants status via increasing GSH, activating SOD and Nrf2 signaling pathway. Results suggested that curcumin has an effective role in counteracting both abnormalities in autophagy process and redox balance, supporting the potential utility of this natural product in effective treatment of NASH.}, keywords = {Curcumin,Nonalcoholic steatohepatitis(NASH) Beclin 1,Oxidative Stress,Nuclear factor erythroid 2-related factor-2 (Nrf2)}, url = {https://besps.journals.ekb.eg/article_8268.html}, eprint = {https://besps.journals.ekb.eg/article_8268_f197255a6ac88717743a5e92d87fc879.pdf} } @article { author = {M. Elsadek, Bakheet and Abdel Aziz, Mohamed and M. El-Deek, Sahar and M. Mahdy, Mohamed and Hussein, Mahmoud}, title = {Combination Therapy with Quercetin and 5-Fluorouracil Ameliorates 1,2-Dimethylhydrazine Induced Carcinogenesis in the Colon of Wistar Rats.}, journal = {Bulletin of Egyptian Society for Physiological Sciences}, volume = {37}, number = {2}, pages = {227-244}, year = {2017}, publisher = {Egyptian Society for Physiological Sciences}, issn = {1110-0842}, eissn = {2356-9514}, doi = {10.21608/besps.2017.8276}, abstract = {Background: Colorectal carcinoma (CRC) is a common cause of cancer-related death worldwide. 5-fluorouracil (5-FU), a first-line chemotherapeutic drug in CRC, has several side effects limiting its therapeutic outcomes. Quercetin (QU) is a dietary bioflavonoid with antioxidant and cytotoxic prooxidant properties. Here, we hypothesize that combination treatment with QU and 5-FU can modulate 1,2-dimethylhydrazine (DMH) induced histological and biochemical changes in the colon of Wistar rats. Methods: A Wistar rats CRC model was established and the animals were randomly divided into five groups. Rats in group A received a suspending vehicle. Group B rats received DMH twice a week subcutaneously for 4 weeks. Animals in the other groups (C, D and E) received the same treatment of DMH, along with QU or 5-FU (individually) or combined QU+5-FU treatment. Results: The DMH-treated rats developed adverse histological alterations (aberrant crypt foci, ACF) and biochemical changes (elevated serum CA19-9; reduced tissue levels of enzymatic antioxidants; elevated CDH12 protein expression and decreased SOX7 mRNA levels). Treatment of DMH-treated animals with QU+5-FU (but not with QU or 5-FU, individually) significantly reversed these changes (i.e., suppressed the formation of ACF; decreased the CA19-9 levels; reduced CDH12 protein expression and increased SOX7 mRNA expression). Conclusions: Conclusively, to the best of our knowledge, our study was the first to evaluate the effects of QU+5-FU treatment on the histological and molecular changes following DMH administration in a rat colon model. Our data suggest that combination therapy with QU+5-FU has therapeutic benefits in colon cancer induced by DMH, with potential for translation to spontaneous disease.}, keywords = {5-Fluorouracil,CDH12,Colorectal carcinoma Dimethylhydrazine,Quercetin,SOX7}, url = {https://besps.journals.ekb.eg/article_8276.html}, eprint = {https://besps.journals.ekb.eg/article_8276_33a8a7823e3c95b6054e178b9d47bd2c.pdf} } @article { author = {elfar, Wesam and Hanafy, Shaden and El-Said, Hala and El-Fert, Ashraf}, title = {Study of MiRNA-155 Gene Expression in Egyptian Patients with Gene Expression in Egyptian Patients with Chronic Hepatitis C Viral infection}, journal = {Bulletin of Egyptian Society for Physiological Sciences}, volume = {37}, number = {2}, pages = {245-259}, year = {2017}, publisher = {Egyptian Society for Physiological Sciences}, issn = {1110-0842}, eissn = {2356-9514}, doi = {10.21608/besps.2017.28976}, abstract = {Objective: to evaluate the miRNA -155 expression in patients with chronic HCV infection & correlate it with hepatitis C viral load and treatment response.Background: MicroRNAs (miRNAs), are small, single-stranded, noncoding RNAs that consist of 20 to 25 base pair (bp).It is a class of small RNAs that regulate mRNA translation and function as oncogenes or tumor suppressor genes. In patients infected with HCV, miRNA-155 expression levels were markedly increased and promote hepatocyte proliferation and tumorigenesis by modulating Wnt signaling.Subjects and Methods: This study was conducted on 20 HCC patients, 60chronic liver disease (CLD) patients due to HCV infection subdivided in to20 patients with HCVnaive treatment,20 patients responder treatment to interferon,20 patients non responder treatment to interferon patients and 20 healthy subjects matching age and gender. Serum AFP was measured for all participants. The relative expression of miRNA-155 was determined in whole blood samples using Real-time polymerase chain reaction.Results: The results revealed over expression of miRNA-155 in each of HCC patient group, patients with HCVnaive treatment and patients non responder to interferon treatment. However, miRNA-155 showed down expression inpatients responder to interferon treatment.miRNA-155 expression was positively correlated with presence of cirrhosis, increased number of focal lesions, larger size of tumor, advanced tumor stage and presence of vascular invasion. From ROC curve analysis, the best cutoff of miRNA-155 chosen to differeniate HCC cases from non HCC subjects was 3.41RQs(Fold expression), and at this point the sensitivity, specificity, +ve predictive value (PPV), -ve predictive value (NPV) and Accuracy were 88.8%,91%, 92.4% ,89.5%,91.4% respectively. For AFP the best cutoff was 85.3ng/ml at this cutoff point the Sensitivity, Specificity , PPV, NPV, Accuracy were (76.2 % , 87.3 % ,, 90.2 % , 72.41 %, 81.0%). Furthermore, combined use of serum AFP and circulating miRNA-155for detection of HCC cases, had the advantage over the use of AFP alone as the sensitivity, specificity, PPV, NPP and overall accuracy were increased (89.32 %, 91.9 %, 93.5%, 90.71 % and 92 % respectively).Conclusion: miRNA-155 expression might be correlated with hepatitis C viral load and treatment response. Also, miRNA-155could be a novel diagnostic and prognostic biomarker for detection of HCC in combination with AFP as well as it could serves as a potential therapeutic target for HCV and HCC infection}, keywords = {Hcc, HCV, MiRNA-155,expression}, url = {https://besps.journals.ekb.eg/article_28976.html}, eprint = {https://besps.journals.ekb.eg/article_28976_cafbb024ae1acad170319b866fd9d385.pdf} }