@article { author = {Dief, Abeer and Mohamed, Adham and Sharara, Gehan}, title = {Changes in Urinary Bladder Smooth Muscle Contractility and the Role of Rho Kinase and Isoprostane after Partial Bladder outlet Obstruction in rats}, journal = {Bulletin of Egyptian Society for Physiological Sciences}, volume = {32}, number = {2}, pages = {1-16}, year = {2012}, publisher = {Egyptian Society for Physiological Sciences}, issn = {1110-0842}, eissn = {2356-9514}, doi = {10.21608/besps.2012.35784}, abstract = {Objective: Bladder outlet obstruction is a common urological problem that associatedwith many cellular changes and remodeling of urinary bladder detrusor smoothmuscle. This study was designed to determine the changes in Rho kinase (ROCK) andisoprostane levels in urinary bladder homogenate after partial bladder outletobstruction (PBOO); and to evaluate the contractile response of isolated rat detrusormuscle after PBOO. Methods: The present work was carried out on 30 male albinorats that were divided into 3 groups (10 rats/group): group I: consisted of normalrats, they were sham operated and considered as control group, group II: consisted ofrats that were undergone PBOO for 14days, group III: consisted of rats that wereundergone PBOO for 28 days. The following parameters were assessed in rats of allgroups: urinary bladder weight, levels of both Rho kinase and isoprostane in thetissues of urinary bladder as well as the contractile response of detrusor muscle bothspontaneously and in response to acetyl choline. Results: The present study revealeda significant increase in the weight of urinary bladder in rats of PBOO of group IIand III as compared to sham operated control of group I. There was a significantincrease in the mean value of Rho kinase and isoprostane in the tissue of the urinarybladder after obstruction. 14 days after obstruction was associated with a significantincrease in the mean value of Rho kinase which was significantly high compared togroup I (sham operated rats) and group III (28days of obstruction), 28 days ofobstruction was associated with decrease in the mean value of Rho kinase comparedto 14 days of obstruction, however, its value was above the control. Regarding thecontractile response, PBOO was associated with increased spontaneous activity ofdetrusor muscle and increased tonic response to acetyl choline which wassignificantly high after 14 and 28 days of obstruction compared to the control.Conclusion: The results of the present study demonstrated that Rho kinase andisoprostane levels were elevated after bladder outlet obstruction and that Rho kinasecould play a crucial role in the regulation of rat urinary bladder smooth musclecontraction and tone. Therefore, Rho kinase inhibitors may prove to betherapeutically useful in the treatment of lower urinary tract disorders associatedwith changes in the physiology of bladder smooth muscle contractility.}, keywords = {Urinary bladder-Obstruction- Contractility- Rho kinase- Isoprostane}, url = {https://besps.journals.ekb.eg/article_35784.html}, eprint = {https://besps.journals.ekb.eg/article_35784_7a79fc46c54409a13e54f2e6d265d903.pdf} } @article { author = {Sabry, Dina and Marzouk, Samar and Hassouna, Amira and Nouh, Olfat and Abdel Aal, Hoda}, title = {Pilot Study on Molecular Quantitation and Sequencing of Endometrial Cytokines Gene Expression and their Effect on the Outcome of In-Vitro Fertilization (IVF) Cycle}, journal = {Bulletin of Egyptian Society for Physiological Sciences}, volume = {32}, number = {2}, pages = {17-30}, year = {2012}, publisher = {Egyptian Society for Physiological Sciences}, issn = {1110-0842}, eissn = {2356-9514}, doi = {10.21608/besps.2012.35789}, abstract = {Background: Human trophoblast invasion and differentiation are essential forsuccessful pregnancy outcome. The molecular mechanisms; however, are poorlyunderstood. Interleukin (IL)-11, a cytokine that regulates endometrial epithelial celladhesion, trophoblast motility and invasion during implantation, may be involved insome of these processes. Leukemia inhibitory factor (LIF) is one of the key cytokinesin the embryo implantation regulation. The present study aimed to assess the levels ofLIF, IL-11 and IL-11 α receptor gene expression in the endometrium of womenundergoing IVF, and correlate their levels with the IVF pregnancy outcome. Also, thestudy aimed to detect any sequence mutation in these three genes among IVFpregnant and non-pregnant women versus control menstrual blood of fertile women.Methods: Endometrial tissue biopsies were taken from 15 women undergoing IVF onthe day of oocyte retrieval. The quantitative expression of IL-11, IL-11Rα and LIFgenes were assessed by real time PCR and PCR products were sequenced by DNAsequencer to detect any DNA sequence changes. Menstrual blood from 10 fertilewomen was used as control to compare the DNA sequence versus DNA sequence ofthe studied genes in endometrial biopsies. Luteinizing hormone (LH), follicularstimulating hormone (FSH) and estradiol (E2) were assessed for enrolled patients byELISA. Endometrial thickness was also assessed by pelvic ultrasonography. Results:No significant difference was detected between quantitative expression of the threestudied genes and pregnancy IVF outcome. Although DNA sequence changes werefound in IL-11 and LIF genes of women with negative pregnancy IVF outcomecompared to women with positive pregnancy IVF outcome. No DNA sequencechanges were detected for IL-11Rα. Other studied parameters (age, LH, FSH, E2 andendometrial thickness) showed no significant differences or correlation withquantitative expression of the three studied involved genes. Conclusions: Datasuggested that no significant differences between quantitative expression of IL-11, IL-11Rα and LIF genes and the IVF pregnancy outcome. The present study may revealthat changes in IL-11 and LIF genes sequence may contribute in pregnancy IVFoutcome.}, keywords = {Gene expression,interleukin-11 (IL-11),interleukin-11 receptor α (IL- 11Rα),leukemia inhibitory factor (LIF),IVF,DNA sequence}, url = {https://besps.journals.ekb.eg/article_35789.html}, eprint = {https://besps.journals.ekb.eg/article_35789_fe044206297efdbc61802c4475512830.pdf} } @article { author = {Mohamed, Amany and Nassar, Ahmed and Mandour, Manal and Saad Eldien, Heba and Mohammed, Ghada}, title = {Effects of Copper (II) Albumin Complex on Thermal Skin Burn}, journal = {Bulletin of Egyptian Society for Physiological Sciences}, volume = {32}, number = {2}, pages = {31-42}, year = {2012}, publisher = {Egyptian Society for Physiological Sciences}, issn = {1110-0842}, eissn = {2356-9514}, doi = {10.21608/besps.2012.35796}, abstract = {Background: Thermal burn results in release of many chemical mediators such asfree radicals, biogenic amines and neurotransmitters. The modulations of thesemediators can enhance burn wound healing.Aim of the Work: To evaluate effects of copper (II) albumin complex on thermalwound healing.Materials and Methods: An animal model of 190 Sprague Dawely rats wasestablished. Ten rats served as a control group and 180 rats were exposed to 10%total body surface area full thickness thermal burn. The rats were equally dividedinto three groups and treated topically either with soft paraffin, moist exposed burnointment (MEBO), or with copper (II) albumin complex. At 4 hours, first day, thirdday, first week, second week and third week post-injury, plasma was used fordetermination of malondialdehyde (MDA), total antioxidant capacity (TAC), 5-hydroxy tryptamine (5-HT) and gamma amino butyric acid (GABA). Skin tissues weretaken for histopathological examination with light microscopy.Results: Thermal injury resulted in a significant increase of plasma levels of MDA, 5-HT and GABA and a significant decrease of TAC. The treated groups with copper (II)albumin complex and MEBO showed a significant decrease of plasma levels of MDAand 5-HT and a significant increase of plasma levels of TAC. Copper (II) albumincomplex treatment was associated with a significant increase of plasma levels ofGABA at the first and third day post-burn as compared to the other treated groups.Characteristic enhancement of regeneration of epidermis and hair follicles wasobserved following application of copper (II) albumin complex.Conclusions: The topical application of copper (II) albumin complex was associatedwith improved healing signs of full thickness thermal burn due to its antioxidant, antiinflammatoryand pain modulating effects.}, keywords = {thermal burn,copper (II) albumin complex,MEBO}, url = {https://besps.journals.ekb.eg/article_35796.html}, eprint = {https://besps.journals.ekb.eg/article_35796_950ded2ebbc8f3a4ce07e1b78a291c7d.pdf} } @article { author = {Soliman, Nema and Keshk, Walaa}, title = {Effect of Porpoise Oil on Kidney of Rats Receiving High Fat Diet}, journal = {Bulletin of Egyptian Society for Physiological Sciences}, volume = {32}, number = {2}, pages = {43-56}, year = {2012}, publisher = {Egyptian Society for Physiological Sciences}, issn = {1110-0842}, eissn = {2356-9514}, doi = {10.21608/besps.2012.35802}, abstract = {Background Obesity is the number one preventable risk factor for chronic kidneydisease. Multiple observational studies have documented an association betweenobesity and risk of kidney disease even after adjustment for obesity-related co-morbidconditions, including diabetes mellitus. Because diet specially dietary fat has beenrecognized as contributing to the development and prevention of obesity, the influenceof quantities and qualities of dietary fat on the pathogenesis of obesity related renaldisorders has been studied. It has been known that tissues of porpoise contain uniquestructured-lipids as combination of iso-valeric acid (iso-C5:0) and ω-3polyunsaturated fatty acids (ω-3 PUFA). It is well known that ω-3 PUFAs have lipidloweringeffects in animal and human studies. Omega3-polyunsaturated fatty acidscan prevent or alleviate obesity-related disorders through the suppression of fattyacid synthesis, enhancement of fatty acid β-oxidation. In the present study the effect ofstructural-lipids from porpoise adipose tissue (porpoise oil) on lipid metabolism wasinvestigated. Design In this study 4 groups of male albino rats (n=10/group): group 1received control diet 10.6% fat, group 2 received high fat diet (HFD) 53.15% fat,group 3 received HFD 53.15% fat with porpoise oil from start of induction of obesityand group 4 received HFD 53.15% fat and followed by addition of porpoise oil for 4weeks after induction of obesity. All groups were subjected to the assay of serumlevels of total lipid profile, urea, creatinine, fasting glucose and insulin as well as thelevels of free fatty acid (FFA), malondialdehyde (MDA) and nitric oxide (NO). Also,renal tissue levels of triacylglycerol (TAG), FFA, and MDA and NO as well as aldosereductase (AR) activity were assayed, with estimation of urinary albumin level.Results: There was statistically significant improvement in all parameters of renalfunction, oxidative stress and dyslipidemia under the effect of porpoise oil intake.Conclusions: The current results might lead us to better utilization of porpoise oildue to its content of ω-3 PUFA to reduce lipid deposition in the kidney tissue withimproving its functions, therefore presenting an opportunity for developing newstrategies to treat obesity.}, keywords = {Obesity,High Fat Diet (HFD),porpoise oil,free fatty acid (FFA),Malondialdehyde (MDA),Nitric oxide (NO),triacylglycerol (TAG),aldose reductase (AR),omega-3 poly unsaturated fatty acids (n-3 PUFA)}, url = {https://besps.journals.ekb.eg/article_35802.html}, eprint = {https://besps.journals.ekb.eg/article_35802_11cfd3f217f58289ffccb9925116787d.pdf} } @article { author = {Idriss, Naglaa and Hassen, Hosny and Sayed, Douaa and Keshek, Yehia and Gaber, Marwa}, title = {Comparison between Circulating Endothelial Cells and Platelet Microparticles Levels in Egyptians Rheumatic Mitral Diseases with and without Atrial Fibrillation}, journal = {Bulletin of Egyptian Society for Physiological Sciences}, volume = {32}, number = {2}, pages = {57-69}, year = {2012}, publisher = {Egyptian Society for Physiological Sciences}, issn = {1110-0842}, eissn = {2356-9514}, doi = {10.21608/besps.2012.35813}, abstract = {Background: Atrial fibrillation (AF) is a continued cardiac arrhythmia, which islinked to a high risk thromboembolism. The association of AF and valvular heartdisease results in a substantial stroke and thromboembolic risk. AF is interrelatedwith a hypercoagulable state and abnormalities of plasma indices of endothelialdamage/dysfunction such as circulating endothelial cells (CECs) and plateletmicroparticles (PMPs), the levels of which are elevated in patients with coronaryartery disease (CAD), diabetes mellitus `and stroke, all of which are conditions thatlead to (AF). Thus, we hypothesized that the levels of CECs and PMPs would beraised in rheumatic atrial fibrillation diseases. Methods: 60 patients with rheumaticvalvular diseases [(30 patients (35±12 years; 10 male and 20 females)] withrheumatic mitral valve disease [(Mitral stenosis MS and Mitral regurgitation MR)]and with normal sinus (RNAF) compared to [(30 patients (31±11 years; 11 male and19 females)] with rheumatic valvular diseases with AF (RAF) and 20 healthy controlsHC (31±10 years; 17 male and 3 females). CECs were calculated using flowcytometry after staining with mouse anti-human CD45 antibody (pan-leukocytemarker), mouse anti-human CD 146 antibody (endothelial cell marker). PMPs weredistinguished from non-platelet events by their fluorescence from Fluorescein 6-isothiocyanate (FITC) conjugated anti-CD41a bound to the particle surfaces.Results: RAF patients had significantly elevated numbers of CECs and PMPscompared to healthy controls HC (P<0.0001). CECs levels were significantly higherin RAF diseases compared to patients with RNAF (P<0.05). RAF patients hadsignificantly higher levels of PMPs (p <0.001) compared to HC. Conclusion: Severeendothelial damage, as assessed by increased numbers of CECs, not to be aprominent trait of rheumatic valvular lesions in sinus rhythm patients, however levelsare definitely high in rheumatic valvular diseases with AF. There is evidence ofplatelet activation in rheumatic AF patients, owing to original cardiovasculardiseases rather than the arrhythmia as such.}, keywords = {Platelet microparticles,Circulating endothelial cells,Atrial Fibrillation,endothelial dysfunction}, url = {https://besps.journals.ekb.eg/article_35813.html}, eprint = {https://besps.journals.ekb.eg/article_35813_feecf5de7380fa33c29af1c6bfaf48fc.pdf} } @article { author = {Tawadrous, Gamil and Mostafa, T and Aziz, Amal}, title = {Seminal Plasma Soluble forms of Fas, Oxidants and Antioxidants in Infertile Men with Varicocele}, journal = {Bulletin of Egyptian Society for Physiological Sciences}, volume = {32}, number = {2}, pages = {71-88}, year = {2012}, publisher = {Egyptian Society for Physiological Sciences}, issn = {1110-0842}, eissn = {2356-9514}, doi = {10.21608/besps.2012.35825}, abstract = {Varicocele is a common cause of male infertility Recent studies at the molecular levelhave demonstrated that varicocele can cause testicular nuclear DNA damage,apoptosis, and raised levels of reactive oxygen species. The present study was carriedout on 120 men: 60 infertile (30 with varicocele and 30 without) and 60 fertile men(30 with varicocele and 30 without). Varicocele was diagnosed clinically and byultrasonography. Standard semen analysis (sperm concentration, motility,morphology; and seminal leucocytic counts) was performed according to the criteriapresented by World Health Organization in 2010. Then, the seminal plasma wasassessed for levels of oxidants [malondialdehyde (MDA)], antioxidants [ascorbicacid, glutathione peroxidase (GPx), catalase (CAT), superoxide dismutase (SOD)]and seminal antiapoptotic factor; soluble fibroblast associated (sFas) which has beenidentified in the testis as a key physiological regulator of apoptosis. The resultsrevealed that patients with varicocele had significantly higher seminal oxidants (MDAP< 0.001) and significantly lower seminal antioxidants (SOD, GPx, CAT andascorbic acid; P < 0.001) and sFas; (P<0.001); as compared to men withoutvaricocele whether fertile or infertile. Patients with grade3 varicocele hadsignificantly higher seminal oxidants, and significantly lower antioxidants and sFasas compared to grade 1 varicoceles (P<0.001). Also, infertile men with or withoutvaricocele had significantly higher seminal oxidants (MDA P<0.001) andsignificantly lower seminal antioxidants (SOD, GPx, CAT and ascorbic acid;P<0.001) and sFas; (P<0.001); compared to fertile men with or without varicocele.Moreover, sperm concentrations in men with varicocele (fertile and infertile showedsignificant positive correlations with the levels of ascorbic acid (r=0.882 &0.626,respectively, P<0.001 for each); with the activity of SOD (r=0.901&0.711respectively, P<0.001 for each), GPx (r=0.909 &0.703 respectively, P<0.001 foreach), CAT (r=0.751&0.679 respectively, P<0.001 for each), and sFas (r=0.750&0.542, P<0.001& <0.005 respectively), and significant negative correlations withthe levels of MDA (r= -0.896 & - 0.732 respectively, P<0.001 for each), In addition,sperm motility showed similar correlations as the sperm concentrations.Conclusions: The present results indicated that varicocele in fertile and infertile menis associated with increased oxidative stress (indicated with significant increase ofMDA), and significant decrease in seminal antiapoptotic factor (sFas) andantioxidants (SOD, GPx, CAT and ascorbic acid). These associations were stronglycorrelated with increased grade of varicocele.}, keywords = {Male infertility,antioxidant,oxidant,Varicocele,apoptosis,sFas}, url = {https://besps.journals.ekb.eg/article_35825.html}, eprint = {https://besps.journals.ekb.eg/article_35825_c4e12b9d23b730761f931740ccb79acc.pdf} } @article { author = {Baalash, Amal and Ibrahim, Bedir}, title = {Diagnostic Implications of Putrescine, Spermidine, and Spermine in Pleural Effusions}, journal = {Bulletin of Egyptian Society for Physiological Sciences}, volume = {32}, number = {2}, pages = {89-100}, year = {2012}, publisher = {Egyptian Society for Physiological Sciences}, issn = {1110-0842}, eissn = {2356-9514}, doi = {10.21608/besps.2012.35831}, abstract = {Background: Pleural effusions are caused by a wide variety of diseases. It isimportant to elucidate their precise etiologies to differentiate benign from malignanteffusions. The polyamines are important molecules governing cell proliferation,survival and apoptosis. Consistent with their elevated levels in cancer, it seemedprobable that patients with active cancer might have elevated levels of thesecompounds in some of their body fluids. The aim of the present study was toinvestigate the diagnostic efficacy of measuring pleural effusion concentration of thepolyamines (putrescine, spermidine, and spermine) for discrimination of malignantand benign pleural effusions. Patients and Methods: Pleural effusions were collectedfrom 138 consecutive patients in whom the diagnosis was confirmed with cytologicaland/or histological examinations. Cytological samples were classified as malignant(n=78) and benign (n=60). Polyamines concentrations were measured using the ionexchange chromatography method. Results: The results showed that the levels of thethree polyamines were significantly higher in malignant pleural effusions whencompared to the benign effusions. Conclusion: The polyamines putrescine,spermidine, and spermine are of great value in the diagnosis of malignancy and maybe used as an adjunct to cytological findings in determining malignant pleuraleffusions.}, keywords = {Polyamines,Pleural effusion,Malignancy}, url = {https://besps.journals.ekb.eg/article_35831.html}, eprint = {https://besps.journals.ekb.eg/article_35831_7278bb39604ecae1f8f76c51f8cf0481.pdf} } @article { author = {Elsamanoudy, Ayman and Selim, Amal and Abdel Aziz, Hussin and Farid, Khaled and Besheer, Tarek}, title = {P53 Codon 72 Gene Polymorphism in Patients with Hepatocellular Carcinoma on Top of Viral and Nonviral Etiologies}, journal = {Bulletin of Egyptian Society for Physiological Sciences}, volume = {32}, number = {2}, pages = {101-114}, year = {2012}, publisher = {Egyptian Society for Physiological Sciences}, issn = {1110-0842}, eissn = {2356-9514}, doi = {10.21608/besps.2012.35838}, abstract = {Background and aim: Loss of p53 function has been suggested to be a critical step inmultistage hepatocarcinogenesis. So, we aimed to investigate the frequency of P53codon 72 gene polymorphism and its relation to plasma P53 levels in Egyptianpatients with hepatocellular carcinoma (HCC) on top of viral and nonviral etiologies.Methods: This is a hospital-based case-control study which included 159 HCCpatients in addition to 83 healthy volunteers as controls. Patients were classified into:63 patients with HCC complicating cirrhosis due to HCV; 55 patients with HCCcomplicating cirrhosis due to HBV and 41 patients with HCC complicating cirrhosisdue to nonviral causes. Quantitative determination of plasma P53 levels wasperformed by ELISA. P53 Arg 72 Pro gene polymorphism was carried out byconventional PCR followed by restriction enzyme digestion (PCR-RFLP). Results:There were significant increases in α.fetoprotein and plasma P53 levels in all studiedgroups in relation to the control group. AA genotype and A allele were more in thecontrol group, PP genotype and P allele were more frequent in HCV related HCCgroup & HCC with non viral causes. AP genotype and P allele were more frequent inHBV related HCC group. P 53 plasma level showed significant increase in all groupsin relation to the control group in AA genotype, AP genotype and PP genotype. Therewere significant increases in AP and PP genotype in all studied groups in comparisonto AA genotype. Plasma P53 level showed significant increase in all groups in bothallele A and allele P when compared with the control group. Also, it showedsignificant increase in their levels in P allele when compared with that of A allele inall studied groups. Conclusion: plasma p53 protein level could be considered as anadditional tumor marker to AFP to increase the diagnostic potential of AFP in HCCpatients. Therefore, P53codon 72 gene polymorphism could be used as an indicatorof the genetic susceptibility for future development of HCC in Egyptian cirrhoticpatients.}, keywords = {P53 codon 72 polymorphism –HCC- plasma P53}, url = {https://besps.journals.ekb.eg/article_35838.html}, eprint = {https://besps.journals.ekb.eg/article_35838_30e281d3a7354b117b36ed917a65fece.pdf} } @article { author = {Ramzy, Maggie and Abdel-Ghany, Hend}, title = {The Effect of Interferon and Ribavirin on Oxidative Stress in Chronic Hepatitis C Patients}, journal = {Bulletin of Egyptian Society for Physiological Sciences}, volume = {32}, number = {2}, pages = {115-124}, year = {2012}, publisher = {Egyptian Society for Physiological Sciences}, issn = {1110-0842}, eissn = {2356-9514}, doi = {10.21608/besps.2012.35841}, abstract = {Hepatitis C virus is the most common cause of chronic viral hepatitis. The hepaticdamage is due to both the cytopathic effect of the virus and the inflammatory changes.Oxidative stress could play a role in pathogenesis of hepatitis C virus infection. Theaim of the present study is to determine oxidant/antioxidant status of patients withchronic hepatitis C and the effect of alpha-interferon plus ribavirin combinationtherapy on oxidative stress. Twenty patients with chronic hepatitis C and ten healthycontrols were included in the study. Erythrocytes reduced glutathione (GSH),superoxide dismutase (SOD) and plasma total sulfhydryl group (tSH) were measuredbefore and after 3 and 6 months of treatment and in control group and correlatedwith serum AST, ALT and viral load. Our results showed that there was reduction inerythrocyte GSH, SOD and plasma tSH in patients with chronic hepatitis C and theybegan to rise significantly early after 3 months of treatment and sometimes normalizeafter 6 months. Also, there were significant negative correlations between theseparameters and liver enzymes (ALT and AST) and viral load. The present studyshowed presence of oxidative stress in chronic hepatitis C patients. Althoughinterferon and ribavirin are not antioxidants, they promote an early inhibition ofoxidative stress. This suggests that antioxidant supplementation might be consideredin patients with chronic hepatitis C.}, keywords = {}, url = {https://besps.journals.ekb.eg/article_35841.html}, eprint = {https://besps.journals.ekb.eg/article_35841_0a6b40b520627870362627c6b473f354.pdf} } @article { author = {Abdel-Karim, Mohammed and Ahmed, Nagwa and Abdel-Mawgoud, Asmaa}, title = {Oxidative Stress in Classic Type Lichen Planus}, journal = {Bulletin of Egyptian Society for Physiological Sciences}, volume = {32}, number = {2}, pages = {125-134}, year = {2012}, publisher = {Egyptian Society for Physiological Sciences}, issn = {1110-0842}, eissn = {2356-9514}, doi = {10.21608/besps.2012.35846}, abstract = {Background: Lichen planus (LP) is an autoimmune inflammatory disease of themucocutaneous tissue, whose exact pathological course is not yet understood. Manystudies have implicated the role of reactive oxygen species (ROS) and the protectiverole of antioxidants in several autoimmune skin disorders. Aim of study: is toevaluate the role of oxidant/antioxidant status in patients with classic type lichenplanus. Patients and Methods: The study included 30 patients of classic type lichenplanus attending Dermatology, Venereology and Andrology Outpatient Clinic inSohag University Hospital, and 20 healthy controls. Serum and tissue levels of nitricoxide(NO),malondialdehyde (MDA), superoxide dismutase(SOD), and catalase (CAT)were measured in patients with classic type lichen planus. Results: The LP patientsshowed statistically significant higher level of serum and tissue NO, MDA and SODcompared to that of controls. On the other hand, patients showed statisticallysignificant lower level of serum and tissue CAT. Male patients showed statisticallysignificant higher levels of serum NO, MDA and SOD than that of female patients. Onthe other hand, male patients had statistically significant lower level of serum andtissue CAT when compared with female patients. On the other hand male patientsshowed statistically significant lower levels of serum and tissue CAT than that of malecontrols. Conclusions: The imbalance between oxidant/ antioxidant mechanisms maybe a primary etiological factor or a secondary possible etiological factor inpathogenesis of classic LP.}, keywords = {}, url = {https://besps.journals.ekb.eg/article_35846.html}, eprint = {https://besps.journals.ekb.eg/article_35846_6e77a631fedf61ace181e1a157217413.pdf} } @article { author = {Aldardery, Samia and Wagih, Ayman and GabAllah, Hanaa and Abdelazeem, Mona}, title = {Impact of Porpoise Oil on Selective Biomarkers Associated with Oxidative Stress in Obesity-Induced in Rats}, journal = {Bulletin of Egyptian Society for Physiological Sciences}, volume = {32}, number = {2}, pages = {135-156}, year = {2012}, publisher = {Egyptian Society for Physiological Sciences}, issn = {1110-0842}, eissn = {2356-9514}, doi = {10.21608/besps.2012.35854}, abstract = {Background: Porpoise oil is yellow fatty oil obtained from porpoises. It causes arelative improvement of antioxidative defense via antioxidant enzymatic and nonenzymatic mechanisms. The antioxidant role of porpoise oil is relevant to itsconstitution of lipid lowering effects of ω-3 polyunsaturated fatty acids) (PUFAs).Obesity has become a leading global health problem owing to its strong associationwith a high incidence of diseases. Oxidative stress may play a critical role in thepathogenesis and development of obesity-related diseases. Aim of the work: The aimof the present study was to evaluate the possible mechanisms of obesity-inducedoxidative stress and to determine the efficacy of porpoise oil on counteractingoxidative stress induced by obesity in rats. Materials and methods: The study wascarried out on 75 female albino rats which were divided into 5 groups each comprise15 rats: group I (control non obese), group II (control obese), group III (non obesewith porpoise oil administration), group IV (porpoise oil administration withinduction of obesity) and group V (porpoise oil administration after induction ofobesity). All groups were subjected to estimation of plasma lipid profile, plasmaxanthine oxidase activity, total antioxidant capacity (TAC), hydrogen peroxide level(H2O2), and malondialdehyde level (MDA). Liver specimens were taken forhistopathological examination. Results: The present study confirmed a state ofdyslipidemia in obese rats manifested by significantly higher plasma levels of totaltriglycerides, total cholesterol, LDL-cholesterol and low levels of HDL-cholesterol ascompared to the control rats. There was significant increase in MDA as well as, H2O2levels, and xanthine oxidase activity, with significant decrease in total antioxidantcapacity in control obese group as compared to the other groups. Porpoise oil treatedgroup showed significant decrease in plasma levels of MDA, H2O2, xanthine oxidaseactivity, with significant increase in TAC. Also, an improvement in lipid profile andlobular architecture of the liver occurred with porpoise oil administration.Conclusion: From these results it could be concluded that obesity is a state ofchronic oxidative stress and may be an inductive factor of several biochemical,metabolic alterations and also hepatic histopathological changes. Porpoise oilsignificantly improves the biochemical, metabolic and histopathologicalabnormalities induced by obesity due to its powerful lipid lowering and antiinflammatoryeffects and unique antioxidant properties.}, keywords = {}, url = {https://besps.journals.ekb.eg/article_35854.html}, eprint = {https://besps.journals.ekb.eg/article_35854_7fdc87c5835dcf81060cc24f9d2dd74f.pdf} } @article { author = {Abedelhaffez, Azza and Metwalley, Kotb}, title = {Impact of Vitamin D Deficiency on Systemic Inflammatory Markers and Left Ventricular Functions in Rachitic Infants}, journal = {Bulletin of Egyptian Society for Physiological Sciences}, volume = {32}, number = {2}, pages = {157-170}, year = {2012}, publisher = {Egyptian Society for Physiological Sciences}, issn = {1110-0842}, eissn = {2356-9514}, doi = {10.21608/besps.2012.35857}, abstract = {Background: Circulating 25 hydroxyvitamin D (25 (OH)D), an accurate measure ofvitamin D status, is markedly reduced in rachitic infants. Aside from the knownrelationship between vitamin D and bone, vitamin D has also been implicated incardiovascular homeostasis, immune function and inflammation. Furthermore, amass of evidence is accumulating that vitamin D deficiency could lead tocardiovascular complications and imbalance of cytokines profile. Our objective wasto study the relationship between vitamin D status (as determined by serum25(OH) Dconcentrations) and inflammatory markers and left ventricular function in rachiticinfants, also to evaluate the effect of vitamin D supplementation on the aboveparameters. Subjects and methods: This study included two groups; vitamin Ddeficiency rickets (VDDR) group (25 infants) and an age matched control group (15infants). After subsiding of the acute illness, the rachitic infants received vitamin Dsupplementation for 6 months. Blood samples were collected in the morning beforethe start of treatment and analyzed for serum 25(OH)D, intact parathyroid hormone(iPTH), Alkaline phosphatase (ALP), calcium (Ca), Phosphorus (Ph) andinflammatory markers [interleukin-6 (IL-6), and C-reactive protein (CRP)].Electrocardiogram (ECG) and echocardiography measuring left ventricular functionswere done. The biochemical variables, ECG and echocardiography were assessed atbaseline and after 6 months of vitamin D supplementation. Results: VDDR group hadsignificant lower 25(OH)D, Ca, Ph and significant higher iPTH, ALP, IL-6 and CRPcompared to the age matched control group at baseline. Echocardiographic findingrevealed significant increase in LVEDD and LVESD and significant decrease in EF%and FS% in VDDR group compared to the age matched control group at the studyentry. Also, ECG finding showed abnormality in some patients at baseline. Thebiochemical, echocardiographic and ECG variables improved significantly after 6months of vitamin D supplementation and reached to those levels found in the agematched control group. Finally, we found negative correlations between 25(OH)Dlevel and IL-6, CRP, LVEDD and LVESD. Also, positive correlations were foundbetween 25 (OH)D and EF% and FS%. These correlations were observed at baselineand after 6 months of vitamin D treatment. Conclusion: VDDR is associated withincreased inflammatory markers and impairment of left ventricular functions inrachitic infants. Vitamin D supplementation ameliorated these effects. Also, resultsgleaned from this investigation support the possible contributing role of the elevatedinflammatory markers in the pathophysiology of left ventricular impairment invitamin D deficiency rachitic infants. More studies are needed to fully characterizethe relationship between Vitamin D induced inflammation and cardiac function inrachitic infants.}, keywords = {Vitamin D deficiency rickets,infants,inflammatory markers,left ventricle function}, url = {https://besps.journals.ekb.eg/article_35857.html}, eprint = {https://besps.journals.ekb.eg/article_35857_abb60dea7bab8a3aa465323f43f7da7a.pdf} } @article { author = {Sayyed, Hayam and Abdel-Hafeez, Azza}, title = {Influence of Endothelin-1 on Age-related Changes in Renal Function in Male Rats}, journal = {Bulletin of Egyptian Society for Physiological Sciences}, volume = {32}, number = {2}, pages = {171-186}, year = {2012}, publisher = {Egyptian Society for Physiological Sciences}, issn = {1110-0842}, eissn = {2356-9514}, doi = {10.21608/besps.2012.35858}, abstract = {The kidney is one of the organs that are highly susceptible to age-related tissuedamage. Several human and animal studies show decline in renal function with age.We examined the effect of endothelin-1 on age-related changes on renal function andwhether this effect is mediated by ET-1 receptor types A or B. Also, we investigatedthe hemodynamic response to ET-1 and ET-1 receptor antagonist. Two age groups ofmale rats were used: young (4–5 months) and old (19–20 months). Each group wassubdivided into four subgroups in which hemodynamic and renal function data weremeasured after administration of saline (control group), acute intravenous injectionof endothelin-1 (ET-1 group), administration of BQ-123, ETA receptor antagonist, 20minutes before ET-1 (ETA antagonist group) and administration of BQ-788, ETBreceptor antagonist, 20 minutes before ET-1(ETB antagonist group). We found thataging is associated with elevated mean arterial blood pressure and reduced renalfunction data. ET-1 injection resulted in more pressor response in old rats and morereduction of renal function data in young rats. Pretreatment with BQ-123 improvedrenal function with more augmentation in old rats while BQ-788 pretreatment has noeffect on renal function. These results indicated that ET-1 and ET-1A receptor play acrucial role in age-related change in renal function.}, keywords = {}, url = {https://besps.journals.ekb.eg/article_35858.html}, eprint = {https://besps.journals.ekb.eg/article_35858_693a5ee5d40fa4e87521666ba3a1cf6d.pdf} } @article { author = {Sallam, Khalid and Abo El-Azm, Tarek and Abadier, Mamdouh and Rageh, Ibrahim}, title = {Combined Estimation of Plasma Cell-free DNA Level and Neuron Specific Enolase Activity as Outcome Predictors of Post-resuscitation Patients}, journal = {Bulletin of Egyptian Society for Physiological Sciences}, volume = {32}, number = {2}, pages = {187-200}, year = {2012}, publisher = {Egyptian Society for Physiological Sciences}, issn = {1110-0842}, eissn = {2356-9514}, doi = {10.21608/besps.2012.35860}, abstract = {Objectives: To evaluate the prognostic yield of estimation of plasma cell-free DNAand neuron specific enolase (NSE) activities in post-resuscitation patients concerningsurvival and neurologic outcome in comparison to Cerebral Performance Category(CPC) score. Patients and Methods: The study included 80 patients developed out-ofhospitalcardiac arrest. All patients received mild therapeutic hypothermiairrespective of the initial rhythm. Blood samples were drawn at study inclusion forestimation of plasma cell-free DNA and serum NSE activity. Mortality rate wasdetermined 1-week and 1-month after admission. Neurologic outcomes wereevaluated using CPC score collectively as CPC score of 1-2 indicated favorableneurological outcome. Results: 1-week mortality rate was 20% and throughout amean ICU stay of 18.4±10.1; range: 3-42 days, another 17 patients died for latemortality rate of 21.3% and a total mortality rate of 46.3%. At time of discharge 31patients (38.8%) were CPC1-2 (Favorable outcome), while 12 patients (14.9%) wereCPC-3 (Unfavorable outcome). Mean plasma DNA and serum NSE activities weresignificantly higher in CPC3 patients compared to CPC1-2 patients. Survivors hadsignificantly lower at admission plasma cell-free DNA and non-significantly lowerserum NSE compared to non-survivors. Survivors had favorable outcome hadsignificantly lower at admission plasma cell-free DNA and serum NSE compared tothose had unfavorable neurologic outcome. There was positive significant correlationbetween plasma cell-free DNA and serum NSE activities and mortality andunfavorable outcome rates. However, the correlation was more significant withplasma DNA than with serum NSE. ROC curve analysis revealed that elevated levelsof both parameters could significantly predict the unfavorable neurologic outcome,while high plasma cell-free DNA could significantly predict high mortality rate.Conclusion: At admission plasma levels of cell-free DNA and serum NSE actsynergistically for prediction of survival and neurologic outcome of post-resuscitationpatients.}, keywords = {Post-resuscitation,Cell-free plasma DNA,Neuron specific enolase,mortality,Neurological outcome}, url = {https://besps.journals.ekb.eg/article_35860.html}, eprint = {https://besps.journals.ekb.eg/article_35860_229a0b778c0ccb0dc22def82bff23c3b.pdf} } @article { author = {Ahmed, Omyma}, title = {Inflammation versus Oxidative Stress in Pathophysiology of Cognitive Dysfunction induced by Aluminum Chloride in Male Rats}, journal = {Bulletin of Egyptian Society for Physiological Sciences}, volume = {32}, number = {2}, pages = {201-222}, year = {2012}, publisher = {Egyptian Society for Physiological Sciences}, issn = {1110-0842}, eissn = {2356-9514}, doi = {10.21608/besps.2012.35861}, abstract = {Alzheimer's disease (AD) is a highly debilitating neurodegenerative disordercharacterized by cognitive dysfunction, inflammation and oxidative stress are thoughtto play major roles in pathophysiology of the AD, which of them have a principle roleis unclear. The role of brain growth factors, cytokines and oxidative biomarkers incognitive dysfunction induced by AlCL3 in rats with application of anti-inflammatory(cilostazol) and antioxidant (N-acetyl cysteine, NAC) were investigated to clarify thepredominant pathophysiological mechanism involved. Methods: Alzheimer's modelgroup was given AlCL3 (100 mg/kg-) orally for 6 weeks. Alzheimer's model + NACand Alzheimer's model + cilostazol groups were given (NAC) and cilostazolrespectively one hr. before AlCL3 for the same duration. Results: anti-inflammatoryor antioxidant significantly improved memory retention which was evaluated byMorris Water Maze, passive avoidance task and eight-arm radial maze. Thisimprovement was consistent with histological recovery and was mediated byreduction AlCL3 concentration in the brain hippocampus and frontal cortex,interference with the cholinergic dysfunction as well as prevention of oxidativedamage. In addition, anti-inflammatory agent can modulate superiorly theinflammatory response via reduction the levels of inflammatory cytokines andadjustment the levels of brain–derived neurotrophic factors and transforming growthfactor B. This finding supports the principal role of inflammation in pathophysiologyof AD and suggests the potential therapeutic application of anti-inflammatory agentfor this condition.}, keywords = {Brain –derived neurotrophic factors (BDNF),transforming growth factor-B (TGF-B),cognitive function test,AlCl3,Cilostazol,Nacetyl cysteine,inflammatory cytokines and oxidative biomarkers}, url = {https://besps.journals.ekb.eg/article_35861.html}, eprint = {https://besps.journals.ekb.eg/article_35861_51deac097f39865690edac047b01f002.pdf} } @article { author = {Ahmed, Omyma and Negm, Eman}, title = {Adipocytokines, growth factors and oxidative markers programmed Alterations in intrauterine growth retardation offspring rats with relevance of melatonin}, journal = {Bulletin of Egyptian Society for Physiological Sciences}, volume = {32}, number = {2}, pages = {223-244}, year = {2012}, publisher = {Egyptian Society for Physiological Sciences}, issn = {1110-0842}, eissn = {2356-9514}, doi = {10.21608/besps.2012.35864}, abstract = {Background: Intrauterine growth restriction (IUGR) is an important perinatalproblem that poses several serious short- and long-term adverse effects on health.Lead is a common environmental and industrial pollutant. Although, a variety ofunfavorable effects of lead may be concerned; the knowledge about its negativedevelopmental effects and its pathophysiological mechanisms remain uncertain. Thisstudy aimed to verify the role of adipocytokines, oxidative markers and growth factorsprogrammed alterations in pathophysiology of IUGR induced by lead in albino ratsalso; to investigate the potential role of melatonin or continuous light or darkness asprophylactic against IUGR. Methods: All pregnant rats except two control groups(saline and melatonin) were given lead in drinking water throughout the pregnancy.Some pregnant rats were co-administered 10 mg/kg melatonin SC while, others wereexposed to lead simultaneously with continuous darkness or light. Half of dams ineach group were delivered spontaneously for following up their weight, pups weights(BW), crown-rump and tail lengths and pups survival rate after 21 days (PND21).The other half of the dams were delivered by laparotomy for detection of placentaweight and lead concentration in placenta. Results: As expected, exposure to leadresulted in decreasing all physical characters of fetus at PND 0 and 21. Of interest,melatonin supplementation or continuous darkness throughout the pregnancyameliorated lead-induced IUGR characters. The counterbalance role of melatoninmay be mediated by a significant adjustment of lead-induced alternations in the levelsof adipocytokines (TNF-a, IL-6 and ghrelin) and growth factors (platelet-derivedgrowth factor, placental growth hormone, transforming growth factor-a and insulingrowth factor-1), nitric oxide and oxidative markers. Therefore, melatonin or itsstimulator may represent a therapeutic option for IUGR and other perinatalcomplications.}, keywords = {}, url = {https://besps.journals.ekb.eg/article_35864.html}, eprint = {https://besps.journals.ekb.eg/article_35864_79ee90f3c78b0e0c2fc3e6070f87c51b.pdf} } @article { author = {Al-Eryani, Ekram and Dughish, Munira and Al-Habori, Molham}, title = {Estimation of Plasma Levels of Zinc, Copper and Selenium in Yemeni Qat Chewer women}, journal = {Bulletin of Egyptian Society for Physiological Sciences}, volume = {32}, number = {2}, pages = {245-254}, year = {2012}, publisher = {Egyptian Society for Physiological Sciences}, issn = {1110-0842}, eissn = {2356-9514}, doi = {10.21608/besps.2012.35908}, abstract = {As no previous study has evaluated zinc, copper, and selenium in Yemeni womenchewing qat, the purpose of this study was to estimate the effects of chewing qat ontheir plasma levels of zinc, copper, and selenium. A total of forty seven healthy, adult,Yemeni women , were selected and grouped in to 24 healthy Yemeni regularlychewing qat, and 23 healthy women non-chewing qat. Plasma samples were collectedfrom two groups for zinc, copper, and selenium analysis by atomic absorption. Theplasma levels (mean ± SD) of zinc, copper, and selenium in Yemeni women chewingqat were 40 ± 610.6 μg/dl, 70±34.6μg/dl, 50±15.2 μg/dl respectively and the mean ±SD of zinc, copper, and selenium in Yemeni women non-chewing qat were 60 ±14.2μg/dl, 40±12.7 μg/dl, and 140±40.6μg/dl. These results, high content of copperand low level of zinc and selenium among women chewing qat can be attributed to theantagonistic effect of qat content as (Cathinone, Copper, Vitamin C, and tannin. Sodeficiency of zinc and selenium among women chewing qat may increase the risk ofdisease.}, keywords = {Cath edulis,Qat,zinc,copper,selenium,Yemen}, url = {https://besps.journals.ekb.eg/article_35908.html}, eprint = {https://besps.journals.ekb.eg/article_35908_a69833c0aef09804820caf7bb90a656c.pdf} } @article { author = {Alsaid, Afaf and El-Missiry, Mohammed and Hatata, El-Sayed and Tarabay, Mohammad and Settin, Ahmad}, title = {Interleukin 4 -590 C>T and Interleukin 13 -1112 C>T Gene Polymorphisms in Relation to the Susceptibility to Type 2 Diabetes Mellitus in Egypt}, journal = {Bulletin of Egyptian Society for Physiological Sciences}, volume = {32}, number = {2}, pages = {255-264}, year = {2012}, publisher = {Egyptian Society for Physiological Sciences}, issn = {1110-0842}, eissn = {2356-9514}, doi = {10.21608/besps.2012.35915}, abstract = {Background: Type 2 diabetes mellitus is the most frequent type of diabetes. It iscaused by insulin resistance and often combined with symptoms of progressive defectin insulin secretion. Various factors have been implicated in the pathogenesis andcomplications of type 2 diabetes mellitus (T2DM), of which, immune response andinflammation were suggested to play a role. Objective: To investigate the associationof IL-4-590 and IL-13 -1112 genetic polymorphisms with (T2DM) in Egyptianpatients. Subjects and Methods: The study included 135 cases with type 2 DM (65males and 70 females), with a median age of 56 years and 101 healthy unrelatedcontrols from Nile Delta region, Egypt. DNA was extracted by purification Capturecolumn kit supplied by Fermentas, K0721, USA. Polymorphisms of IL-4 -590(C>T)and IL-13 -1112(C>T) genes were characterized using ARMS-PCR technique.Results: The frequency of heterozygous (CT) genotype of IL-4-590 was significantlyincreased in type 2 diabetic patients compared to the control, CT genotype of IL-13-1112 was highly significantly increased in diabetic patients compared to control (85.2vs.66.3, OR=2.9, p=0.001; 76.3 vs.51.5, OR=3' p= 0, respectively). The frequency ofthe homozygous (CC) genotypes of both IL-4 and IL-13 were significantly lower intype 2 diabetic patients than in the control group (10.4 vs. 26.7, OR=0.32, p=0.002;20.7 vs 31.7, OR=0.6, p=0.04, respectively). The frequency of CT+TT genotypes ofIL-4 gene were higher in patients compared to controls (89.6 vs. 73.3, OR =3.2, P=0.002).Results did not show any significant difference between allele frequency indiabetic and healthy controls either. Conclusion: Polymorphisms related to IL-4-590CT and IL-13-1112 CT genotypes may be considered as a risk factor for type 2diabetes mellitus among Egyptian subjects.}, keywords = {IL4 polymorphism,IL13 polymorphism,Type 2 diabetes mellitus,Egypt}, url = {https://besps.journals.ekb.eg/article_35915.html}, eprint = {https://besps.journals.ekb.eg/article_35915_ea6b936b20a7918aef07afe1868faefc.pdf} } @article { author = {Mahmoud, Ahmed and Ali, Basma}, title = {Cystatin C as an Early Marker of Glomerular Dysfunction in Children with Beta Thalassemia Major}, journal = {Bulletin of Egyptian Society for Physiological Sciences}, volume = {32}, number = {2}, pages = {265-278}, year = {2012}, publisher = {Egyptian Society for Physiological Sciences}, issn = {1110-0842}, eissn = {2356-9514}, doi = {10.21608/besps.2012.35922}, abstract = {Background and aim of work: Reports investigating renal dysfunction in betathalassemia major (β-TM) patients have been limited in number, mainly studyingadult patients. Additionally, most of them had not assessed early markers ofglomerular dysfunction such as cystatin C. Early identification of patients at high riskis of great importance as it may allow specific measures to be taken to delay renalimpairment. The present work aimed to estimate the frequency of glomerulardysfunction in children with β-TM by using different markers and to correlate thesemarkers to serum ferritin and iron chelation therapy. Patients and Methods: Thestudy included one hundred patients with β-TM (Group Ι) which was subdivided into;groups: I-a included 62 patients (62%) with iron chelation therapy (deferoxamine)and group I-b included 38 patients (38%) without iron chelation therapy and GroupII (control group) included fifty apparently healthy volunteers age and sex matched tothe diseased groups. Members of the two groups were subjected to; history taking,clinical examination and laboratory investigations including determination of: Serumferritin, albumin/creatinine ratio in urine, eGFR by both Schwartz formula andcreatinine clearance; blood urea and serum creatinine and finally serum cystatin C.Results: Group I showed significant higher levels of Cystatin C, serum creatinine,serum ferritin, albumin /creatinine ratio in urine than group II. Furthermore, theyhad significantly lower eGFR and creatinine clearance than group II (p<0.05).Moreover, group Ι-a had significant lower eGFR and creatinine clearance than groupI-b. Also, cystatin C had highly significant strong negative correlation with eGFR andcreatinine clearance and significant strong positive correlation with serum ferritin.Finally, cystatin C had higher sensitivity and specificity than serum creatinine andcreatinine clearance for small changes in GFR. Conclusion: Glomerular dysfunctionin β-TM is not a rare complication so, the use of early markers such as cystatin C isuseful for the early detection of small changes in GFR. Periodic renal assessment ofthose patients is mandatory where many of them may had hidden renal affection}, keywords = {Glomerular dysfunction,β-Thalassemia,Cystatin C}, url = {https://besps.journals.ekb.eg/article_35922.html}, eprint = {https://besps.journals.ekb.eg/article_35922_26902b5c7c53ecb0bc5b68ca00db46fa.pdf} } @article { author = {Elkazaz, Amany and Salama, Khaled}, title = {The Effect of Oral Contraceptive: Different Patterns of Use on Circulating IGF-1 and Bone Mineral Density in Healthy Premenopausal Women.}, journal = {Bulletin of Egyptian Society for Physiological Sciences}, volume = {32}, number = {2}, pages = {279-292}, year = {2012}, publisher = {Egyptian Society for Physiological Sciences}, issn = {1110-0842}, eissn = {2356-9514}, doi = {10.21608/besps.2012.35926}, abstract = {Background: Both insulin like growth factor-1(IGF-1) and oral contraceptive (OC)use have been linked to premenopausal breast and colorectal cancers, osteoporosisand cardiovascular disease. Understanding the effects of different patterns of use ofOC on IGF-1 levels and bone mineral density (BMD) may offer insight into itsinfluence on osteoporosis. Methods: We conducted a cross-sectional study of 135women who were included into 3 groups (Group A= OC users, 43 women; Group Bwho never use OC, 51 women; and Group C who were past users, 41 women). Eachpatient completed a questionnaire on demographic parameters, marital state history,and contraception history including duration of use and type of contraceptive pills orused method. Lower end radius, proximal femur and lumbar spine BMD weremeasured by dual-energy X-ray absorptiometry. IGF-1 was assessed withchemiluminescent immunometric assay. Results: The 3 groups were matched for ageand BMI, and nearly similar in total body T- value of BMD (with slight better resultsin past-users than the other 2 groups but it was statistically insignificant difference),but the other BMD values shows significant difference between the studied groupsregarding the measurement at lumbar spine and femur which were statisticallysignificantly better results in Group C (past users). Among past-users women themean level of circulating IGF-1 was higher than the other 2 groups, and thatdifference was statistically significant. Conclusion: Lower IGF-1 level amongcurrent users may also potentially lead to decreased BMD, while the higher levels weobserved in older past users may decrease the osteoporosis risk, reflecting observedrelationship between IGF-1, BMD, and oral contraceptives.}, keywords = {IGF-1,BMD and Oral contraceptives}, url = {https://besps.journals.ekb.eg/article_35926.html}, eprint = {https://besps.journals.ekb.eg/article_35926_e04361d9eedbbee100be180344beddb9.pdf} } @article { author = {Ismail, Emad and El Kazaz, Amany and ElSerafi, Ahmed and Abd El All, May and Nasr, Gamela}, title = {Role Of Insulin Like Growth Factor-1 and Insulin Like Growth Factor Binding Protein-3 In Patients Of Coronary Artery Disease Attending Suez Canal University Hospital}, journal = {Bulletin of Egyptian Society for Physiological Sciences}, volume = {32}, number = {2}, pages = {293-308}, year = {2012}, publisher = {Egyptian Society for Physiological Sciences}, issn = {1110-0842}, eissn = {2356-9514}, doi = {10.21608/besps.2012.35932}, abstract = {Cardiovascular disease is the leading cause of mortality, not only in the Westernworld, but also in developing countries. It has been suggested that circulating insulinlike growth factor-1 (IGF-I) and insulin like growth factor binding protein-3(IGFBP-3) are involved in the pathophysiological processes underlying coronary arterydisease. We evaluated the relationship between the levels of IGF-I and IGFBP-3 ndcoronary atherosclerosis in sixty two patients attending Cardiac Clinic and CoronaryCare Unit at Suez Canal University Hospital, and 30 healthy controls. IGF-1 andIGFBP-3 were measured using Immulite 1000. Fasting blood glucose and lipidprofile were measured for all subjects. Coronary artery disease was more frequentamong males compared to females (67.7%, and 32.3% respectively). Total cholesterol(TC), low density lipoprotein cholesterol (LDLc) and triacylglycerol (TAG) levelswere significantly higher, while HDL was significantly lower in patients thancontrols. The means of IGF-1 and IGFBP-3 were significantly higher among controlgroup compared to patients group (212.5 ± 109.1 ng/ml and 6.4 ± 2.3 ng/ml versus129.5 ± 66.2 ng/ml., 4.4 ± 1.6 ng/ml respectively, p= 0.001 for each). Thirty (48.4%)of patients had 3 stenosed vessels. The mean percent of stenosis was 74.7 ± 14.2 %.IGF-1 and IGFBP-3 were lower in the group of three affected vessels but there wasno significant correlation between both parameters and severity of the disease. Therewas significant correlation between IGF-1 and HDL (r =0.254, p-value = 0.046) inpatients group while no significant correlation between IGF-1 and other parametersin both groups. There was no significant correlation between IGFBP-3 and otherparameters in both groups. In conclusion, IGF-1, IGFBP-3 may be used to determinethe risk of coronary artery disease in Egyptian population living in Suez Canal area.}, keywords = {IGF-1,IGFBP-3and CAD}, url = {https://besps.journals.ekb.eg/article_35932.html}, eprint = {https://besps.journals.ekb.eg/article_35932_fc661a736d5144262cd69f26311351c9.pdf} } @article { author = {Habib, Salem and Eissa, Laila and Abdel Latif, Mohammed}, title = {Potent Effect of Superoxide Dismutase -Like Activity of Partially Purified Protein from Raphnus Sativus Roots on Transforming Growth Factor and Vascular Endothelial Growth Factor In Ehrlich Ascites Carcinoma Bearing Mice}, journal = {Bulletin of Egyptian Society for Physiological Sciences}, volume = {32}, number = {2}, pages = {309-326}, year = {2012}, publisher = {Egyptian Society for Physiological Sciences}, issn = {1110-0842}, eissn = {2356-9514}, doi = {10.21608/besps.2012.35939}, abstract = {The alterations in cancer cell's metabolism have been associated with enhancedoxidative stress. Therefore, a partially purified SOD-like activity protein from theroots of Raphnus sativus (R. sativus) and low-molecular-weight heparin (LMWH)were tested for their abilities to inhibit the growth of Ehrlich ascites carcinoma cells(EAC) intraperitoneally implanted in albino mice. Ninty mice were randomly dividedinto 6 groups. The antitumor effect of the partially purified protein and LMWH wereassessed by estimating transforming growth factor Beta (TGF-β) and vascularendothelial growth factor (VEGF) in serum. Also, tumor volume, median survivaltime (MST), total lipids, DNA and RNA contents in liver tissues as well as liverfunction tests and the redox status were estimated. TGF-β, VEGF, DNA, RNA, andmalondialdehyde (MDA) levels in addition to serum alanine transaminase (ALT) andgamma glutamyl transferase (GGT) activities and tumor volume were highlysignificantly increased (P<0.001) in the untreated EAC-bearing mice compared tothose of the control. However, total lipids in liver tissues and serum albumin werehighly significantly decreased in the same group compared to the correspondingvalues of the control group. All these parameters were restored to their normal levelsin the partially purified extract and LMWH treated EAC-bearing mice groupscompared to the untreated EAC-bearing mice. It could be concluded that, the partialpurified protein with SOD-like activity from the roots of R. sativus and the LMWHhave a remarkable anti-tumor activity against EAC cells in Swiss albino mice throughits potent effect on both TGF-β and VEGF.}, keywords = {Transforming growth factor-β,vascular endothelial growth factor,Ehrlich Ascites Carcinoma (EAC),Low-molecular-weight heparins,Raphnus sativus,and SOD-like activity}, url = {https://besps.journals.ekb.eg/article_35939.html}, eprint = {https://besps.journals.ekb.eg/article_35939_367cc7c381af31b1ae780e33e73a74cb.pdf} }