eng
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
2010-12-01
30
1
1
20
10.21608/besps.2010.36162
36162
Original Article
Renal Functions in Pediatric Patients with β-Thalassemia Major: Relation to Iron Chelation Therapy
Enas Hamed
eah3a2010@yahoo.com
1
Nagla ElMelegy
2
Department of Physiology , Faculty of Medicine, Assiut University.
Department of Medical Biochemistry, Faculty of Medicine, Assiut University.
Renal failure is one of the main complications in β-thalassaemia as a result of longstandinganemia, therefore the objectives of the study were: (1) To investigateglomerular and tubular functions in transfusion dependant (TD) β-thalassaemiamajor (βTM) pediatric patients without any occult renal diseases. 2) To correlate thefindings with clinical parameters, oxidative stress status [by measuring serum totalantioxidant capacity (TAC) and urinary malondialdehyde (MDA)] anddesferrioxamine (DFO) chelation therapy. Patients and methods: The study includedsixty-nine TD-βTM patients (45 males and 24 females). They were subdivided intothose with (34 patients) and without chelation therapy (35 patients). In addition tofifteen age-, sex-, body mass index (BMI)-matched healthy subjects as a controlgroup. From each participant blood sample was taken for determination of the serum(S) levels of creatinine (Cr), albumin, calcium (Ca), inorganic phosphorus (PO4), uricacid (UA), cystatin-C (CysC) and TAC. Also a urine sample was taken fordetermination of urinary (U) levels of creatinine, albumin, N-acetyl-beta-Dglucosaminidase(NAG) activity, β2-microglobulin (β2MG) and MDA. Results: Theresults revealed that in βTM patients the serum levels of Cr, albumin, PO4, UA, CysC,and urinary levels of NAG/Cr, β2MG/Cr, MDA/Cr, albumin/Cr were significantlyhigher; while serum TAC, estimated glomerular filtration rate (eGFR) weresignificantly lower than those of the controls. In patients with chelation therapy,serum levels of CysC, and albumin were significantly higher while, TAC wassignificantly lower than those without chelation therapy. Significant positivecorrelations were observed in TD-βTM patients between SCysC and each of Salbuminand SCr; STAC and eGFR and UNAG/Cr with each of Uβ2MG/Cr, UMDA/Cr andUalbumin/Cr. Also, significant negative correlations were found between SCysC andeGFR; STAC and each of SCys, SCr, and Salbumin. Conclusion: The results of thepresent study confirm that renal tubular dysfunction exists in children with βTMwhich could be attributed to iron overload, oxidative stress and DFO therapy.
https://besps.journals.ekb.eg/article_36162_a32dba85a3f65c4c4019ecf71261a5f0.pdf
β-thalassaemia major
β2-microglobulin
Cystatin-C
Desferioxamine
Nacetyl- beta-D-glucosaminidase activity
Oxidative Stress
eng
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
2010-12-01
30
1
21
36
10.21608/besps.2010.36163
36163
Original Article
Assessment of the Role of Maternal Angiogenic Factors and Nitric Oxide in Prediction of Preeclampsia
Olfat Shaker
1
Fatma Taha
2
Salwa Fayez
3
Mohmmed Shehata
4
Dalia Ahmed
5
Department of Medical Biochemistry , Faculty of Medicine, Cairo University
Department of Medical Biochemistry , Faculty of Medicine, Cairo University
Department of Medical Biochemistry , Faculty of Medicine, Cairo University
Department of Obstetrics & Gynecology , Faculty of Medicine, Cairo University
Department of Public Health & Community Medicine , Faculty of Medicine, Cairo University
Preeclampsia has been proposed to be an antiangiogenic state that may be detected by the determination of the concentrations of the soluble vascular endothelial growth factor receptor- 1 (sVEGFR-1) and placental growth factor (PlGF) in maternal blood even before the clinical development of the disease. Aim: The aim of the present study was to determine the role of the combined use of uterine artery Doppler velocimetry (UADV) and maternal plasma PlGF , sVEGFR-1 and NO products concentrations for the prediction of preeclampsia in high-risk women.and to compare these parameters between patients with mild and severe preeclampsia. Subjects and Methods: A prospective cohort study was conducted on 142 women, only 112 were enrolled in the study, patients with preeclampsia were subclassified as either severe or mild preeclampsia. Blood samples were obtained between 22 and 26 weeks of gestation. Doppler ultrasound of the uterine arteries at the time of blood sampling was done. The presence of an early diastolic notch in the uterine arteries was determined. An abnormal UADV was defined as the presence of bilateral uterine artery notches and/or a mean pulsatility index above 95th percentile for the gestational age. Maternal serum PlGF and sVEGFR-1 concentrations were determined with the use of sensitive and specific immunoassays. Nitric Oxide Colorimetric Assay was used also to measure NO products in the maternal blood. Results: Among patients with abnormal UADV, maternal plasma sVEGFR1, PlGF and NO products concentrations contributed significantly in the identification of patients destined to develop mild preeclampsia and severe preeclampsia sVEGFR1 (>2005 pg/ml) and NO products (<50.90 umol/L) were found to be the best predictors for preeclampsia with high sensitivity and specificity followed by PLGF (<286.32 pg/ml). In severe preeclampsia sVEGFR1 (>2900 pg/ml) was the best followed by NO products (<54 umol/L) and PLGF (<234.56 pg/ml). Conclusion: The results of current study suggested that the identification of high concentrations of sVEGFR1 combined with low concentrations of PlGF and NO products, could be used to predict the development of preeclampsia.
https://besps.journals.ekb.eg/article_36163_8ad7028228127eb836117684c4a41422.pdf
preeclampsia
soluble vascular endothelial growth factor receptor- 1 (sVEGFR1)
placental growth factor (PlGF)
Nitric oxide (NO)
eng
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
2010-12-01
30
1
37
52
10.21608/besps.2010.36164
36164
Original Article
Gly82Ser Polymorphism of the Receptor for Advanced Glycation End Products Gene (RAGE) and the Endogenous Secretory RAGE (esRAGE) and its Association with Diabetic Retinopathy in Type 2 Diabetic Patients
Yousri Hussein
1
Etewa L
2
Bahaa El Din Hassan
3
Faculty of Medicine. Zagazig University. Medical Biochemistry Department
Faculty of Medicine. Zagazig University. Medical Biochemistry Department
Faculty of Medicine. Zagazig University. Opthalmic Department
The binding of advanced glycation end-products (AGEs) to their receptor (RAGE)may play an important role in the development of diabetic retinopathy (DR). Recently,endogenous secretory RAGE (esRAGE) has been identified as an alternative splicingform of RAGE able to capture AGEs, and exerts protection against AGEs-inducedendothelial cell injury. A Gly82Ser polymorphism in exon 3 of RAGE gene wasidentified and thought to have an effect on the functions of its protein. This study wasplanned to investigate the frequency of the Gly82Ser polymorphism in RAGE geneand the role of esRAGE as a biological marker for DR in type2 diabetes and itsassociation with the severity of DR. Thirty-five patients with type2 diabetes wererecruited into the study. They were subclassified into 15 patients with no clinicallyapparent retinopathy (No DR), 12 patients with nonproliferative DR (NPDR), and 8patients with proliferative DR (PDR). Twenty, age matched, healthy subjects wereincluded as controls. Serum esRAGE level was measured by enzyme-linkedimmunosorbent assay. Genotype frequencies of Gly82Ser polymorphism were studiedby polymerase chain reaction amplification and restriction fragment lengthpolymorphism analysis using AluI enzyme. The results showed no significantdifference between serum esRAGE levels in both controls and diabetic patients withNo DR (P = 0.15). Among the diabetic subjects, there was a significant decrease ofserum esRAGE levels between patients with No DR and patients with NPDR (P =0.008) and a more significant decrease between diabetic patients with No DR andpatients with PDR (P = 0.001). The low serum esRAGE diabetic patients had higherrisk to develop DR than those with high serum esRAGE level (odds ratio = 4.7, 95%confidence interval = 1.07-20.65, P = 0.02). There were no significant differences ingenotyping frequencies or allele frequencies between controls and diabetic patientswith No DR, or patient with DR (P<0.05). In conclusion, serum esRAGE level showeda significant association with the severity of DR and, hence, it could be used as aprognostic tool to predict the development and progression of DR. esRAGE could bea novel and potential protective factor for DR. Gly82Ser polymorphisms in RAGEgene are not associated with the susceptibility of type 2 diabetes, or with thedevelopment of DR in type 2 diabetic subjects.
https://besps.journals.ekb.eg/article_36164_49d9c0ad97f4593594703260291f2bca.pdf
eng
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
2010-12-01
30
1
53
72
10.21608/besps.2010.36165
36165
Original Article
Role of Interleukin-18, S-adenosylmethionine and SAdenosylhomocysteine as Cardiovascular Risk Factors in Patients with Systemic Lupus Erythematosus
Ayman El Samanoudy
1
Adel Zalata
2
Abd El Hameed Metwali
3
Fayez el Kenawy
4
Adel Elbadrawy
5
Department of Medical Biochemistry, Faculty of Medicine, Mansoura University
Department of Medical Biochemistry, Faculty of Medicine, Mansoura University
Department of Internal medicine, Faculty of Medicine, Mansoura University
Department of Internal medicine, Faculty of Medicine, Mansoura University
Department of Diagnostic Radiology, Faculty of Medicine, Mansoura University
Background: The incidence of Systemic Lupus Erythematosus (SLE) appears to beincreasing and the main cause of death in that disease is coronary artery diseasesince SLE is associated with premature atherosclerosis. The association of plasmainterleukin-18 levels and proinflammatory cytokines with cardiovascular risk in SLEpatients has not been extensively established. Hyperhomocysteinemia is associatedwith increased risk for cardiovascular events, but it is not clear whether it is a markeror mediator for vascular dysfunction or a marker for another risk factor. Aim of thework: The purpose of the present study was to determine whether plasma IL-18, SAM,SAH and SAM/SAH ratio are associated with cardiovascular risk factors and diseaseactivity in SLE patients. Subjects and Methods: The plasma concentrations of a novelpro-inflammatory cytokine, interleukin (IL)-18 by ELISA as well as SAM,SAH andSAM/SAH ratio by HPLC was determined in 31 patients with systemic lupuserythematosus (SLE) and 30 sex- and age-matched healthy control subjects andcorrelated them with cardiovascular risk factors and the SLE disease activity. Forevery patient the systemic lupus disease activity was assessed using the SystemicLupus Erythematosus Disease Activity Index (SLEDAI). Body mass index (BMI),systolic blood pressure, diastolic blood pressure, CBC, liver functions, plasmacreatinine, urine analysis, erythrocyte sedimentation rate (ESR)1, ANA, anti- ds DNA,C3, C4, fasting insulin and glucose, plasma lipid profile, plasma SAH,SAM,SAM/SAH ratio, titers of autoantibodies against oxidized low-density lipoprotein andcarotid intima media thickness (CIMT) were determined. SLE patients with a historyof diabetes mellitus, hypertension, hyperlipidemia, smoking, or coronary arterydisease (CAD) and positive pregnancy test were excluded. Results: The mean age ofSLE patients was 35.1±10.3 years and the mean duration of SLE was 4.2± 2.9 years.Plasma concentrations of IL-18 were significantly higher in SLE patients than agematchedhealthy controls (p< 0.001). Also, plasma SAH is elevated in SLE patientsversus controls while SAM and SAM/SAH ratio were significantly lower in SLEpatients versus controls. Elevation of plasma IL-18 correlated positively andsignificantly with SLE disease activity index. In addition, plasma concentrations ofIL-18 correlated positively and significantly with BMI, insulin, Homeostasis modelassessment insulin resistance (HOMA IR), triglycerides, CIMT, and SAH, in SLEpatients. IL18 concentrations showed a positive and significant correlation with
plasma creatinine (r=0·7, P = 0·001), antinuclear antibody (ANA) (r= 0.6, p=0.001),anti double stranded DNA (dsDNA) (r=0.5, p=0.008), ESR1 (r= 0.56, p=0.001). Theconcentrations of plasma IL-18 in SLE patients with elevated plasma creatinine weresignificantly higher than those with normal plasma creatinine (285.7.6±59.6 pg/ml vs182.8±29.4, p< 0.001). Also, SLEDAI correlated positively with both plasma levels ofinsulin and HOMA-IR values (p<0.05 in both). Conclusions: In SLE patients, a highIL-18 level reflects activity of the disease and is related to cardiovascular risk factors.IL-18 is therefore suggested to play a crucial role in triggering the inflammatoryprocesses of premature atherosclerosis in SLE, in addition to the markers ofdisturbed homocysteine metabolism could play a role as mediator of cardiovasculardisorders in SLE.
https://besps.journals.ekb.eg/article_36165_fe5ccdd8ff640a667b14004e282cf5c2.pdf
IL 18
SAH/SAM ratio
Systemic lupus erythematosus
disease activity
Atherosclerosis
T-helper cell cytokines
eng
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
2010-12-01
30
1
73
102
10.21608/besps.2010.36166
36166
Original Article
The Role of Urinary Fibronectin and Telomerase in Patients with Superficial Bladder Cancer after Transurethral Resection Treated with BCG
Ghada Abdel Aleem
1
Hala Hamouda
2
Tarek Gameel
3
Mona Abdel-Azem
4
Medical Biochemistry Department, Faculty of Medicine, Tanta University
Medical Biochemistry Department, Faculty of Medicine, Tanta University
Urology Department, Faculty of Medicine, Tanta University
Pathology Department, Faculty of Medicine, Tanta University
Objective: Bacillus Calmette-Guérin (BCG) has been shown to be an effective treatment for superficial transitional cell carcinoma (TCC) of the bladder, but the precise mechanism of action of BCG remains poorly understood. Fibronectin (FN) has been found to play a role in BCG therapy. Although adjuvant BCG has been shown to improve the clinical outcome of superficial bladder cancer (SBC), a significant proportion of patients fail to respond to it. Identification of the subset of patients that are going to develop recurrent disease or stage progression after BCG therapy is very important. Telomerase activity represents a potential tool for tumor detection. The aim of the present study was to give an insight into the diagnostic and prognostic values of the quantitative urinary estimation of both fibronectin (FN) and the catalytic subunit of the complex human telomerase reverse transcriptase [hTERT]) as non invasive tumor markers for early detection of patients with high risk superficial transitional cell carcinoma (TCC) and after trans-urethral resection of bladder tumors (TURBT) that received adjuvant BCG immunotherapy to explore its association with recurrence-free-survival (RFS) or development of invasive disease. Materials and methods: The study was carried out on 10 healthy individuals as a control group (group I) and 20 Egyptian patients with histologically confirmed superficial bladder cancer (group II). Patients were underwent formal TURBT. Morning urine samples were collected from every patient, 1 day before and 2days, two weeks, six weeks, three months, six months and one year after TURBT. Also, morning urine samples were collected from the healthy individuals. Urine samples were used for cytological examination, estimation of fibronectin level using ELISA technique and for the quantitative analysis of hTERT using quantitative real time RT-PCR technique. Results: The results showed that urinary fibronectin level and normalized hTERT- mRNA were significantly higher in SBC patients before TURBT (group II) versus control group (P<0.001). hTERT-mRNA and urinary fibronectin levels were significantly different in group II before and 2 days after TURBT. Urinary fibronectin and normalized hTERT- mRNA were significantly lower 2 weeks and 6 weeks after TURBT than patients group 2 days after TURBT. Both markers were significantly lower in fifteen patients 3 months, 6 months and one year after TURBT when compared with their levels 2 days after TURBT. There was insignificant difference of both markers between the control group and fifteen patient one year after TURBT. The remaining five patients suffered recurrence of tumor. This approach enabled us to identify cutoff values of urinary fibronectin which characterized by 90% sensitivity and 100% specificity and 93.3% accuracy, and that for urinary normalized hTERT with 85% sensitivity and 80% specificity and 83.3% accuracy. Conclusion: On the basis of these results, it could be concluded that both urinary fibronectin and hTERT- mRNA can be considered from the best markers for diagnosing superficial bladder carcinoma. These parameters can be also used to determine the presence of residual tumor load after TURBT; also they can give an insight about the expected response to BCG immunotherapy after TURBT and detect the BCG nonresponder patients. It seems conceivable that these emerging urine-based tests may progressively replace urine cytology and form a reliable adjunct to cystoscopy in the diagnosis and follow up of patients with superficial bladder cancer.
https://besps.journals.ekb.eg/article_36166_2c3d16c32476e334f4fd7db8040e1d3b.pdf
Bacillus Calmette-Guérin (BCG)
Superficial bladder cancer (SBC)
Fibronectin (FN)
transitional cell carcinoma (TCC)
carcinoma in situ (CIS)
human telomerase reverse transcriptase (hTERT)
transurethral resection of bladder cancer (TURBT)
eng
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
2010-12-01
30
1
103
122
10.21608/besps.2010.36167
36167
Original Article
Some Potential Biochemical Markers of Leprosy
Ghada Abdel Aleem
1
Hoda Moneib
2
Sahar Youssef
3
Nahla Darwish
4
Mona Abdel-Azem Mona Abdel-Azem
5
Medical Biochemistry Department, Faculty of Medicine, Tanta University
Dermatology & Venereology Dep; Faculty of Medicine, Ain Shams University
Dermatology & Venereology Dep; Faculty of Medicine, Ain Shams University
Dermatology & Venereology Dep; Faculty of Medicine, Ain Shams University
Pathology Dep., Faculty of Medicine, Tanta University
Background: Leprosy is a chronic granulomatous infectious disease caused by thebacterium Mycobacterium leprae. Leprosy "Type 1" reactions (T1R), reversalreactions, occur in 30–40% of borderline patients with cellular immune responses toM. leprae. "Type 2" reactions (T2R), also known as erythema nodosum leprosum(ENL), occur only in lepromatous (LL) and borderline lepromatous (BL) patients witha high bacterial load and little or no cellular immunity to M. leprae. Corticosteroidsalleviate symptoms in T1R and T2R, but many patients have multiple, recurrentepisodes. The Objective of the present study is to verify the validity of measuringchitotriosidase activity and neopterin level, products of activated macrophages,adenosine deaminase activity and monocyte chemoattractant protein-1 (MCP-1) asmarkers of leprosy and to detect their values in diagnosis of different types of leprosy.Methods: This study was conducted on 15 healthy subjects and 75 leprotic patientsthat were classified into 5 groups [tuberculoid leprosy (TT), borderline tuberculoid(BT), borderline borderline (BB), borderline lepromatous (BL), and lepromatousleprosy (LL)], each group formed of 15 patients, depending on clinical,bacteriological and histopathological pattern. Patients were further grouped with aBI≥2 as multibacillary (MB, n=45), whereas those with BI<2 were grouped aspaucibacillary (PB, n=30). Thirty-four of the aforementioned patients werediagnosed with reactions of which 17 had type II/erythema nodosum leprosum (ENL)and 17 had type I/reversal reaction (RR). Reactions were treated using prednisolonefor 12 weeks. Venous blood sample was collected from each subject and processed forestimation of the activity of chitotriosidase and adenosine deaminase, neopterin andMCP-1 levels. Results: both chitotriosidase activity and neopterin level were elevatedin leprosy patients with significant elevation in MB than PB leprosy with significantlowering in the patients with reactional leprosy after prednisolone therapy.Adenosine deaminase activity was significantly elevated in TT, PB and reactionalleprosy. MCP-1 was significantly elevated in LL, MB and ENL. Conclusion:chitotriosidase and neopterin could be considered as promising markers fordifferentiation of MB from PB patients and useful for determining the response ofreactional leprosy to therapy. Adenosine deaminase could be useful in distinguishingTT, PB and reactional leprosy. MCP-1 could be considered a fair marker in LL, MBand ENL. In addition, these findings may provide new clues to the pathogenesis ofleprosy reactions.
https://besps.journals.ekb.eg/article_36167_90fd0e683b9debbb986b34d871e59b8a.pdf
multibacillary (MB)
paucibacillary (PB)
Non-reactional leprosy (NR)
erythema nodosum leprosum (ENL)
reactional leprosy (RL)
reversal reaction (RR) and healthy controls (HC). Tubercloid Leprosy (TT)
Leprometous Leprosy (LL)
Bordreline Lepromatous (BL)
Bordreline Tubercloid (BT)
Bordreline Bordreline (BB)
eng
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
2010-12-01
30
1
123
136
10.21608/besps.2010.36168
36168
Original Article
Effect of Histidine on Autotaxin Activity in Experimentally Induced Liver Fibrosis
Manal El-Batch
1
Wafaa Ibrahim
2
Soha Said
3
Medical Biochemistry Department, Faculty of Medicine, Tanta University
Medical Biochemistry Department, Faculty of Medicine, Tanta University
Medical Biochemistry Department, Faculty of Medicine, Tanta University
The aim of the present study was to explain whether serum autotaxin activity might bea target for regulation of liver fibrosis and to evaluate the hepato-protective and antifibroticeffects of histidine in thioacetamide induced liver fibrosis in rats. The studywas carried out on 100 albino rats, classified into 5 groups each of 20 rats: group І(control group), group ІІ: rats were given histidine intra-peritoneally, group ІІІ: ratswere injected intra-peritoneally with thioacetamide, group IV: rats were injected withL-histidine together with thioacetamide, group V: rats were injected with TAA for onemonth then treated with intra-peritoneal injection of L-histidine for another month. Atthe end of experiment, blood and liver were collected for determination of some liverenzymes, plasma total antioxidant capacity, serum autotaxin activity and liver tissuehydroxyproline. Thioacetamide treatment caused significant increases in liverenzymes, autotaxin activities and liver hydroxyproline, but with a significant decreasein plasma total antioxidant capacity. Upon treatment with histidine significantdecreases in liver enzymes, autotaxin activities and liver hydroxyproline wereobserved with a significant increase in plasma total antioxidant capacity in group IVand significant decrease in group V. Conclusion: histidine as an antioxidant has aprotective effect on thioacetamide-induced liver fibrosis , its beneficial effects in ratsmight be not only by inhibition of collagen synthesis and increasing total antioxidantcapacity, but also by inhibition of autotaxin activities, thus reducing its capacity toproduce lysophosphatidic acid which has a role in liver fibrosis.
https://besps.journals.ekb.eg/article_36168_d6c6825fcd2917018696af46cecebf21.pdf
autotaxin
histidine
Liver fibrosis
Thioacetamide
total anti-oxidant capacity
eng
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
2010-12-01
30
1
137
148
10.21608/besps.2010.36169
36169
Original Article
Biochemical Studies on the Molecular Level of Polioviruses
Hend El Sherbini
1
Naglaa Abd El Meguid
2
Clinical Pathology Department, Faculty of Medicine, Cairo University
The Holding Company for Biological Products and Vaccines (VACSERA)
Paralytic poliomyelitis continues to be an important disease affecting many children.The strategy for poliomyelitis eradication includes routine immunization, surveillanceof acute flaccid paralysis (AFP) and mopping-up activities. Environmentalmonitoring, mainly involving the screening of waste water can provide evidence ofthe circulation or absence of wild-type polioviruses. The present work has beencarried out to investigate the sequence divergence of neurovirulent derivatives of theSabin oral poliovirus vaccine (OPV) strains, to establish epidemiological linkbetween isolates, and to determine poliovirus genotype which circulates in Egypt.Enzyme Linked Immunoassay (ELIZA) and Reverse Transcription Polymerase ChainReaction (RT-PCR) for intratypic differentiation have been used. Molecular analysisof the VPI/2A junction region has been performed on 22 isolates using the dideoxychain termination method. Sequencing of the amplified region has been performedusing the automated genetic analyzer ABI prism 310. Forward and reverseelectropherograms were compared using sequencing alignment software version 2.Phylogenetic analysis of the VPI/2A region was done using Clustal X software.
https://besps.journals.ekb.eg/article_36169_3790caccac133746e305dc5c515d6e27.pdf
Sequence divergence
neurovirulent
epidemiological link
genotype
VPl/2A junction region
Phylogenetic analysis
eng
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
2010-12-01
30
1
149
158
10.21608/besps.2010.36170
36170
Original Article
Identification of A 38 KDa Antigen in the Urine of Schistosoma Mansoni Chronicaily Infected Patients Using A Specific Antibody Microeluted from A Nitrocellulose Membrane; A Diagnostic Tool
Samir Mahgoub
1
Department of Biochemistry, Faculty of Medicine, Al-Minia University
Schistosomiasis is a chronic debilitating disease affecting 200 -300 millionthroughout the world, a major focus of research has been done to identify andcharacterize antigens that may have vaccine and/or diagnostic potential. In thepresent study, NP-40 extracted surface proteins of Schistosoma mansoni (S.mansoni) adult worms were subjected to 12.5% Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis (SDS-PAGE) and electrotransfered onto anitrocellulose membrane (PVDF), then, incubated with pooled sera collected from S.mansoni chronically infected patients. The presence of antibodies identify a numberof NP-40 extracted surface proteins of the adult worms of S. mansoni. One of theseproteins of 38 kDa molecular weight with high immunogenicity was selected. Thestrip of nitrocellulose membrane containing the complex of the identified protein andits specific antibody was cut guided by the molecular weight marker, then, theantibody was micro-eluted. Proteins of urine samples from the same patients wereprecipitated and purified over G-Sephadex column. The purified proteins of urinesamples and proteins of sera were subjected to another SDS- PAGE and electrotransferredonto PVDF membrane. The microeluted antibody was used to identify anantigen of 38 kDa molecular weight in sera of the chronically infected patients aswell as in urine. The 38 kDa antigen was excreted in the urine of those patients in astable form and detected by the specific monoclonal antibody. The active epitope of38 kDa antigen could be a promising immunochemical probe for S. mansoniinfection diagnosis. Further studies will be done to characterize that antigen as wellas its potential application in immunodiagnosis for S. mansoni
https://besps.journals.ekb.eg/article_36170_d388d53329cb1ee5ce6d1917910c98a6.pdf
eng
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
2010-12-01
30
1
159
170
10.21608/besps.2010.36171
36171
Original Article
Molecular Characterization of the Gene Encoding SJCHGC 03921 Protein of the Lung Stage of Schistosoma Mansoni (7-Days Schistosomula)
Samir Mahgoub
1
Department of Biochemistry, Faculty of Medicine, Al-Minia University
The parasitic helminth Schistosoma mansoni (S. mansoni) is a major public healthconcern in many developing countries. Over 200 million people have, and another600 million are at risk of contracting schistosomiasis which is one of the majorneglected tropical diseases. For this dangerous disease the development of long -lasting immunity through vaccination may be the real solution to control the spreadof the disease. The molecules on the surface or associated with the tegument of S.mansoni are a major focus as potential vaccine candidates. In the present study, allsurface and internal proteins of the lung stage of the parasite were screened toincrease the chances for the discovery of a unique protein of the parasite to betargeted by the immune system of the host. Pooled sera were collected from S.mansoni chronically infected patients, then, purified over a column made of solubleextract of the lung stage (7-days schistosomula) of S. mansoni. The eluted antibodieswere used to immunoscreen λgt11 cDNA library of 7-days schistosomula. A numberof cDNA clones were identified after three rounds of immunoscreening and plaquespurification. The phage DNAs of the isolated clones were amplified by polymerasechain reaction (PCR) using λgt11 forward and reverse primers, then, cloned inPCRTMII plasmid vector. The isolated clone 4-65 was fully sequenced and was foundencoding the gene of SJCHGC 03921 protein of 7-days schistosomula of S. mansoni.Also, the 0.9 kb cDNA clone was found to have a single open reading frame (ORF)encoding 269 amino acids, which exhibited 94% homology with the gene of SJCHGC03921 protein of Schistosoma japonicum.
https://besps.journals.ekb.eg/article_36171_7853ebaf3768780050d08a891edc6adb.pdf
eng
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
2010-12-01
30
1
171
184
10.21608/besps.2010.36172
36172
Original Article
Schistosoma Mansoni: Partial Molecular Characterization of the Gene Encoding Zinc Finger Protein of the Lung Stage (7-Days Schistosomula)
Samir Mahgoub
1
Department of Biochemistry, Faculty of Medicine, Al-Minia University
Schistosomiasis is a serious parasitic disease with world-wide distribution, causingan estimated 200 000 deaths per year. Despite the fact that the global distribution ofschistosomiasis has changed significantly in the past 50 years, particularly in regionswhere control strategies have been successfully employed, the disease remainsendemic in over 70 developing countries and more than 200 million people areestimated to be harboring the disease. Chemotherapy, although effective, it does notprevent re-infection, and in addition, partial drug resistance may occur. Hence,immunological intervention in the form of a vaccine would contribute to the successof the present efforts. Most of the trials in the development of anti-schistosomiasisvaccine were involving membrane-associated antigens contained in the adultSchistosoma mansoni (S. mansoni) tegument because they are capable ofstimulating protective immunity. In the current study, a trial to obtain antigenvarieties which could be vaccine candidates by incorporating internal antigens of thelung stage of S. mansoni (7-days schistosomula) in addition to the tegumentalantigens was done. The soluble extract of the lung stage was obtained by sonicatingthe whole parasite, then, coupled to Sepharose-4B column for affinity purification ofpooled sera collected from chronically infected patients. The purified sera were usedto immunoscreen λgt 11 cDNA library of 7-days schistosomula. The plaquespurification after three rounds of immunoscreening gave a number of cDNA clones.one of the isolated clones (clone 2-4) was amplified by PCR using λgt 11forward and reverse primers, then ,cloned in a plasmid vector (PCRTMII). Thecloned insert was partially sequenced 270 bp from the 5/- end using Sp6 primer aswell as 187 bp from the 3/-end using T7 primer. The sequenced part of the cloneshowed it has two open reading frames (ORFs) with 31-36% homology to the genethat encodes Zinc Finger protein (the transcriptional regulatory protein) from anumber of eukaryotic species including human , rat and mice.
https://besps.journals.ekb.eg/article_36172_2cb77f452c0779c6b3dbc53d4a6fcb50.pdf
eng
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
2010-12-01
30
1
185
206
10.21608/besps.2010.36173
36173
Original Article
Renal Actions of Neutral Endopeptidase Inhibition and Its Effects on Gene Expression of Atrial Natriuretic Peptide and Neutral Endopeptidase in Rats with Chronic Heart Failure
Ayman Elsamanoudy
1
Amr Abbas
2
Department of Medical Biochemistry , Faculty of Medicine, Mansoura University, Egypt
Department of Medical Physiology, Faculty of Medicine, Mansoura University, Egypt
Background and Aim of work: The aim of the current study is to evaluate the effectsof acute and chronic inhibition of NEP, by ONO-9902, on ANP, and NEP geneexpressions, hemodynamic and renal parameters in rats with chronic heart failure(CHF) following left coronary artery ligation (CAL). Methods: The study comprised48 male Sprague-Dawley rats (220–240 g) which were divided into sham and CALgroups. Myocardial infarction was induced by left CAL. All rats were divided intountreated and orally treated with ONO-9902 (300 mg/kg/day) from the 1st to 6th weekafter the operation At the 1st and 6th weeks after the operation, gene expression ofANP and NEP, plasma ANP, cGMP, and aldosterone concentrations, urine volume,Na and ANP excretion, creatinine clearance, renal cGMP generation, body andorgan weight were measured. Results: CAL led to sodium and water retention,increased plasma level of ANP and aldosterone, in addition to increase in ANP geneexpression as well as decrease in renal generation of cGMP. Acute treatment of ratswith CAL by NEPI, at the first week after the operation, inhibited the NEP geneexpression with increased plasma ANP concentration and gene expression, whichcaused diuresis and natriuresis and increased renal cGMP generation. Moreover,chronic treatment by NEPI caused significant decrease in lung weight, lung bodyweight ratio, NEP gene expression and PAC, non significant increase in plasmaconcentration and gene expression of ANP, diuresis and natriuresis with increasedrenal cGMP generation. GFR is not significantly changed either before or aftertreatment. Conclusions: It is concluded that gene expression and plasma level ofANP increased in CHF. Also, chronic treatment with NEP inhibitor improvespulmonary edema and decreases Na and water retention in rats with CHF bydeceasing degradative effect of NEP on ANP which leads to prolongation of itsbioactivity. So, ONO-9902 may offer a new therapeutic approach in patients withCHF.
https://besps.journals.ekb.eg/article_36173_d6b44d023eb62ac158a073acf4905650.pdf
Heart failure
kidney
atrial natriuretic peptide
neutral endopeptidase
aldosterone
Rats
eng
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
2010-12-01
30
1
207
220
10.21608/besps.2010.36174
36174
Original Article
The Effect of Aspirin on Survivin and COX-2 Expression and Their Correlation in Aberrant Crypt Foci
Maggie Ramzy
1
Soha Abdelwahab
2
Biochemistry Department, Faculty of Medicine, Minia University
Histology Department, Faculty of Medicine, Minia University
Non steroidal anti-inflammatory drugs (NSAID) such as aspirin havechemopreventive activity against colorectal tumors. Although the molecularmechanism has not been fully established, it is thought to involve COX-mediatedcarcinogen activation, cell proliferation, apoptosis and immune surveillance. In thepresent study, the effect of aspirin on the expression of survivin and COX-2 and theirpossible correlation in induced colorectal carcinoma were investigated. We used 1, 2dimethylhydrazine (DMH) for induction of aberrant crypt foci (ACF) that contributeto the stepwise progression to colorectal cancer. The rats were divided into 3 groups;control group, DMH group and DMH with aspirin group. The expression of survivinand COX-2 was investigated through IHC and immunoblot and the correlation inboth DMH-induced lesions and after aspirin treatment was evaluated. It was foundthat aspirin has the ability to reduce the incidence of the ACF when it was given as achemopreventive and it was found that both survivin and COX-2 are overexpressed inACF compared to normal group. They seem to be early events in the occurrence ofcolorectal carcinoma. In the evaluation to the effect of aspirin it was found thataspirin significantly causes down-regulation of both survivin and COX-2 (P=0.0001).Furthermore, we found survivin and COX-2 to be positively correlated in ACF lesionand after aspirin treatment (r = 0.816 and r = 0.742) respectively. All of thesefindings suggested that targeted therapies against survivin and COX-2 may bevaluable in chemoprevention of colorectal carcinoma.
https://besps.journals.ekb.eg/article_36174_32d5c344fcdf713fd006b302df72b5b9.pdf
aspirin
aberrant crypt foci
survivin
Cyclooxygenase-2
eng
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
2010-12-01
30
1
221
232
10.21608/besps.2010.36175
36175
Original Article
Protective Effect of Nigella Sativa in Alloxan Induced Diabetic Rats
Ahmed Mahmoud
ahmed_physiology@yahoo.com
1
Bahaa Abdel- Salam
2
Wafaey Gomaa
3
Biochemistry Department, Faculty of Medicine, Minia University
Zoology Department, Faculty of Science, Minia University
Pathology Department, Faculty of Medicine, Minia University
Oxidative stress has been suggested to be a contributory factor in complication ofdiabetes mellitus. There are many reports indicating the change in parameter ofoxidative stress in alloxan induced diabetic rats, we aimed to find if the oxidativestress which occurs in pancreas has a role in development of diabetes mellitus. Thiswas done by measuring plasma lipid profile, insulin, interleukin 8 (IL-8),transforming growth factor α and reduced glutathione content in pancreas. The studywas carried on 30 rats, classified into 3 groups: control group, diabetic group inwhich diabetes was induced by a single intraperitoneal (i.p) injection of alloxanmonohydrate (120 mg/kg of body weight), treated group in which rats were givenNigella sativa extract i.p. (0.2 ml/kg/day), 2 week before injection of alloxan, theseinjections were continued daily until the end of the study (for 4 weeks). The resultsshowed significant increase of plasma glucose, total cholesterol, triglycerides, lowdensity lipoprotein cholesterol (LDLc), interleukin 8 and transforming growth factorα in diabetic group, with significant decrease in high density lipoprotein cholesterol(HDLc), reduced glutathione content in pancreas, and insulin level in diabetic group.The treated groups showed significant reduction in plasma glucose, total cholesterol,triglycerides, LDLc, interleukin 8 and transforming growth factor α with significantincrease in HDLc, reduced glutathione content in pancreas and insulin level. It couldbe concluded that Nigella sativa treatment for 1.5 months in rats with experimentallyalloxan-induced diabetes increase the activity of the anti-oxidant defense system.Thus, it could be used as an anti-diabetic complement in cases of diabetes mellitus.
https://besps.journals.ekb.eg/article_36175_bb6c56332e2725b4b8a2739b9d416512.pdf
Alloxan and Nigella Sativa in rats
eng
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
2010-12-01
30
1
233
254
10.21608/besps.2010.36176
36176
Original Article
Protective Effect of Melatonin on Carbon Tetrachloride - induced Hepatic Fibrogenesis in Rats
Mona El-Karn
1
Medical Physiology Department, Faculty of Medicine, Assiut University
Background: Liver cirrhosis is a critical stage of chronic liver diseases that canproduce liver failure, portal hypertension and hepatic carcinoma. Sustained oxidativestress plays a key role in cell damage and fibrosis induced during liver cirrhosis. Aimof the work: The aim of the present study was to examine the potential protectiveeffect of exogenous melatonin co-treatment on liver tissue injury and oxidative stressprocesses during induction of early phase of liver fibrosis by carbon tetrachloride(CCl4) injection in rats. Methods: Hepatic fibrogenesis model was induced in thisstudy by subcutaneous injection of rats by carbon tetrachloride (CCl4). Eighteenadult, female albino rats were randomly divided into 3 groups (n = 6): control group(group I), carbon tetrachloride treated group (group II) and CCl4 + melatonin cotreatedgroup (group III). Rats in CCl4 treated group were injected subcutaneouslywith sterile CCl4 (2 ml/kg of body weight) in a ratio of 1:1 with olive oil twice a weekfor 8 weeks. Rats of group III (melatonin co-treated group) were injected with CCl4 inthe same manner as in group II and received intraperitoneal melatonin injection in adose of 20 mg/kg twice a week for 8 weeks, starting from the beginning of CCl4injection. Rats in normal control group were injected subcutaneously with olive oil atthe same dose and frequency as those in CCl4 treated group. At the end of theexperiment, rats were sacrificed, blood samples were collected for biochemical assay.Liver from each animal was removed for histopathological examination.Measurement of oxidative stress markers in serum was done by chemical estimationof serum levels of free radicals: lipid peroxides (LPO) and nitric oxide (NO).Antioxidant enzymes were estimated by chemical measurement of glutathioneperoxidase (GSH-Px) and superoxide dismutase (SOD) in the serum. Liver injury wasassessed by evaluation of serum levels of liver enzymes (alanine aminotransferase(ALT), and aspartate aminotransferase (AST)). Determination of development ofearly phase of hepatic fibrogenesis was done by chemical measurement of serum levelof hyaluronic acid (HA) using enzyme immunoassay (ELISA), and byhistopathological examinations of hepatic tissues to detect early fibrotic changes aswell as other histological damage of hepatic tissue caused by CCl4 injection with orwithout melatonin administration. Results: Results of the present study showed thatCCl4 treatment to rats of group II caused highly significant increase in serum levelsof oxidative stress markers (lipid peroxides and nitric oxide), decrease in serum levelsof antioxidant markers (glutathione peroxidase and superoxide dismutase), increaseof serum levels of hepatic enzymes (ALT and AST) as well as increased serum level ofhyaluronic acid (HA) 8 weeks after CCl4 injections when compared with controlgroup. Melatonin co-treatment to animals of group III caused significant reduction in
serum levels of lipid peroxides (LPS) and nitric oxide (NO), significant increase inplasma levels of glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD),significant reduction in serum levels of liver enzymes (ALT and AST) as well assignificant decrease in serum level of hyaluronic acid (HA) 8 weeks after CCl4injections when compared with group II. Histopathological study of liver tissue ofanimals of CCl4 treated group showed various manifestation of hepatic cell damageand early phase of fibrogenesis as necrosis, degeneration, collagen deposition andfew fibrous threads extending into the hepatic lobules. Histopathological study ofhepatic tissue of melatonin co-treated group showed that melatonin caused markedamelioration of histological manifestations of hepatic cell degeneration and absenceof any sign of fibrogenesis with nearly normalization of the histological appearanceof the hepatic tissue. Compared with CCl4 treated group (group II), histologicalappearance of hepatic tissue of rats in melatonin co-treated group (group III) showedsignificant improvement. Conclusion: Results of this study suggest that melatonin hasa substantial hepatoprotective effect in a rat hepatic fibrosis model induced by an 8-weeks’ CCl4 regimen. The protective effect of melatonin may be due to both its directradical scavenging properties and indirect effect as a regulator of antioxidantsystems. Therefore, the study proposes that melatonin may be a valuable drug forinhibition of unwanted fibrosis in patients exposed to different hepatotoxic agents.
https://besps.journals.ekb.eg/article_36176_01a6af77d0ce0c6574edcfe17a3abab6.pdf
eng
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
2010-12-01
30
1
255
274
10.21608/besps.2010.36177
36177
Original Article
Impact of Obesity in School Age of Late Childhood in Helwan Governorate
Afaf Mohsen
1
Safaa Ismail
2
Community Health Nursing,, Faculty of Nursing –Helwan University
Pediatric Health Nursing, Faculty of Nursing –Helwan University
Background: Childhood obesity is an emerging global public health challengebecause of the great increase in the prevalence of obesity among children andadolescents in all parts of the world. In Egypt, the prevalence of obesity amongchildren and adolescent was 14.7 %and 15.08 %for males and females respectively.In addition, the incidence of overweight and obesity among children is slowlybecoming a world-wide problem in developed and developing countries .There is nodoubt that the percentages are even greater nowadays because of physical inactivityand westernization in diet. In all times and in all cases, prevention is better than cure.So, health care providers need to take a proactive role when treating children andfocus on prevention of obesity rather than waiting until the condition exists. Aim ofstudy: is to assess life style habits which lead to obesity for school age children andevaluate its effect on puberty in school age children. Method: This is a descriptiveresearch design; it was carried out in Ezbet Elwalda in Helwan governorate. Subjectswere all obese students and accepted to participate in the study from both sexes,aged10- 12 years old ( late childhood stage), 13- 17 years old ( the adolescent stage)and Marley juvenile diabetes were excluded and their total number was 216.Theresearchers utilized self administered interview sheet which included the following ,personal information, eating habits, life style pattern and gender puberty andphysical assessment (height , weight & BMI).All data collected from first of March tothe end of April 2010. Results: The majority of studied sample had boys, theirnumbers was 138 from 216 student. The majority of studied sample were prefer to eatfast food on weekly basis (41.7%), drink beverage and eat candy (70.8%), take snacksbetween meals (52.8%) as ice cream in (66.7%) and eat potato chips (60.2%). Threequarter of obese sample had not seeking medical advice and 71.4 % were physicallyinactivity. All obese girls had pubic hair, armpit hair, developed nipple of the breastand 40 % of them started menarche at eleven years old. Otherwise there is nosignificant relation between overweight and obese boys regarding developed testes,emergence of armpit hair, developed growth hand and foot, masculine voice.Recommendation: Health care providers need to play a preventive role whentreating children and focus on prevention of obesity rather than wait until thecondition exists. Also, referring client to an experienced registered dietitian to obtaina full nutritional assessment for the child and family.
https://besps.journals.ekb.eg/article_36177_1feadee6478102ee7e36e6e794067285.pdf
obese
overweight
BMI
eng
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
2010-12-01
30
1
275
286
10.21608/besps.2010.36178
36178
Original Article
A Study of the Role of Some Antioxidants in Diabetes Induced in Rats
Nagy Tawfek
1
Mahmoud Elrehany
2
Adel Basyouny
3
Hanaa Hassan
4
Department of Zoology, Faculty of Science , El-Minia University, El-Minia, Egypt
Departement of Biochemistry, Faculty of Medicine, El-Minia University, El-Minia, Egypt
Department of Zoology, Faculty of Science , El-Minia University, El-Minia, Egypt
Department of Zoology, Faculty of Science , El-Minia University, El-Minia, Egypt
Oxidative stress is produced under diabetic conditions and possibly causes variousforms of tissue damage and destruction of pancreatic β-cells in insulin-dependentdiabetes mellitus (IDDM) patients. The present study was carried out to examine theinvolvement of oxidative stress in the progression of pancreatic β-cell dysfunction intype 1 diabetes and to evaluate the potential usefulness of antioxidants in thetreatment of type 1diabetes. The present study was achieved using ٢٤ male SpragueDawley albino rats. Rats were divided into three groups: normal control rats,diabetic control rats, and diabetic rats received mixture of antioxidants. A mixture ofantioxidants (N-acetyl-cysteine (NAC), alpha-lipoic acid (LA), vitamin E and vitaminC) was orally administered daily to cyclophosphamide-induced diabetic rats for aperiod of two months. The results revealed that these antioxidants exertedamelioration in fasting plasma glucose level, significant inhibition of lipid peroxideslevel and observed elevation in glutathione (GSH) level and glutathione peroxidase(GPX) activity of diabetic rats. On the basis of the present results it could beconcluded that (N-acetyl-cysteine (NAC), alpha-lipoic (LA), vitamin E and vitamin C)restored the activities of the studied parameters and the activities of some enzymes indifferent ways, depending on special mechanism in each one. Supplementation ofantioxidants at once after diagnosis of diabetes may delay the complications ofdiabetes. This finding suggests a potential usefulness of antioxidants for treatingdiabetes and provides further support for the implication of oxidative stress in β-celldysfunction in diabetes.
https://besps.journals.ekb.eg/article_36178_e2920d55e887f014de4824a7de8c5b39.pdf
eng
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
2010-12-01
30
1
287
300
10.21608/besps.2010.36179
36179
Original Article
Role of N-Acetylcystein in Protection against Cisplatin Nephrotoxicity
Sahar Habib
1
Maggie Ramzy
2
Manal Abd Elghany
3
Forensic Med. And Toxicology Department, Faculty of Medicine, Minia University
Biochemistry Department, Faculty of Medicine, Minia University
Pathology Department, Faculty of Medicine, Minia University
Background: Cisplatin (CDDP) is a cytotoxic therapeutic agent that causes manyphysiological adverse effects such as nephrotoxicity. Although N-acetylcystein (NAC)a thiol containing antioxidant, has been documented to be effective in attenuatingCDDP induced renal injury, the precise mechanisms involved in its renoprotectionhave not been completely clarified. Methods: Four groups of albino rats were used.The first group injected by saline as control, the second by NAC (i.p.), the third by asingle bolus of cisplatin (i.v.), and the last by NAC/CDDP, after 5 days from CDDPinjection, investigations were conducted on the levels of serum urea and creatinine,oxidant/antioxidant status by estimation of malondialdehyde, catalase and reducedglutathione, and renal and systemic tumor necrosis factor-alpha (TNF-α). Also, renaltissues were examined histopathologically. Results: Administration of cisplatin to ratsinduced a significant increase in serum urea and creatinine levels in addition tosevere alterations in renal tissue architecture. Cisplatin also increasedmalondialdehyde and decreased glutathione, and catalase in renal tissues. Also,CDDP increased both renal and systemic TNF-α. Administration of NAC markedlyreduced the cisplatin-induced higher serum creatinine and urea levels andcounteracted the effects of cisplatin on oxidative stress markers, protected the tissuesfrom the cisplatin-induced lipid peroxidation, and increased TNF-α and improvedrenal tissue architecture. Conclusion: NAC protection is mediated by preventing thedecline of antioxidant status, inhibit malondialdehyde and TNF-α and preventnecrosis and apoptosis from kidneys. These results have implications in use of NAC inhuman application for protecting against drug-induced nephrotoxicity.
https://besps.journals.ekb.eg/article_36179_23923bfc4e05142356f45b018e6fad25.pdf
Cisplatin
Nephrotoxicity
reduced glutathione
Malondialdehyde
catalase
NAC
TNF- α
apoptosis
eng
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
2010-12-01
30
1
301
310
10.21608/besps.2010.36180
36180
Original Article
Bcl2 and c-erbB-3 as Prognostic Markers of Hepatocellular Carcinoma
Hanan Fouad
1
Ahmed Eldemery
2
Medical Biochemistry Department, Faculty of Medicine, Cairo University
Biochemistry Department, Faculty of Medicine, October 6 University
The present study was conducted to evaluate gene expression profile of c-erbB-3 andbcl2 in hepatocellular carcinoma patients (HCC) with and without associatedhepatitis C virus (HCV) infection. Forty eight subjects were included in the study anddivided equally into 2 groups: Group 1 involved HCC subjects with associated HCVinfection and group 2 involved HCC subjects without HCV associated infection.Adjacent para-cancerous tissues were examined as control samples. Correlationswith various clinico-pathological parameters of the tumor were assessed; stage,tumor size, intra-hepatic metastasis and carcinoma differentiation. c-erbB-3oncogene was expressed in 83.33% (40/48) of total HCC samples and in 31.25%(15/48) of the noncancerous lesions. c-erbB-3 was expressed in 87.5% (21/24) ofHCC samples with associated HCV infection and in 79.16% (19/24) of HCC withoutassociated HCV infection. Gene expression of c-erbB-3 was significantly correlatedto the clinico-pathological parameters of the tumor. As regards bcl2 gene expression,the gene was expressed in 20.8% (10/48) of total HCC samples and in the paracancerouslesions. Bcl2 was expressed in 20.8% (5/24) of HCC samples with andwithout HCV associated infection. Gene expression of bcl2 did not show significantcorrelations to the clinico-pathological parameters of the tumor. In conclusion,expression profile of c-erbB-3 in hepatocellular carcinoma patients could be used asa prognostic molecular marker in HCC.
https://besps.journals.ekb.eg/article_36180_2e6aa55c1e7204435881aca355c756dd.pdf
Hepatocellular carcinoma
Bcl2
c-erbB-3