ORIGINAL_ARTICLE
The Role of Hydrogen Sulfide in Chronic Unpredictable Stress-Induced Gastric Lesions.
The aim of this study was to investigate the possible protective role of Hydrogen sulfide on chronic unpredictable stress (CUS) -induced gastric lesions. Materials and methods: 40 rats were divided into 4 groups: control group, stressed rats group, stressed + Aminooxyacetic acid (AOAA) (inhibitor of H2S synthesis; 50mg/kg/48hour; IP), stressed + Sodium hydrosulfide (NaHS) (donor of H2S, 5mg/kg/48hour; IP). In all the groups exposed to CUS, a set of chronic unpredictable stressors was applied for 6 weeks in random order. At the end of experimental protocol blood samples, gastric content and gastric tissues samples were collected. Gastric tissues histopathological changes were evaluated by macroscopic and microscopic examination. Gastric content pH, serum MDA level, whole blood GSH level were estimated. Expression of Caspase-3 (apoptotic marker) and BCL-xl (anti-apoptotic marker) was assessed by immunohistochemistry. Results: In all the groups exposed to CUS, there was a significant reduction in gastric pH value and a range of gastric lesions ranging from superficial to deep ulceration as evident by macroscopic and microscopic examination. Also, there was significant elevation in MDA serum level and significant reduction in anti-oxidant GSH blood level. In all stressed rats, the expression of Caspase-3 was significantly higher whereas a significant decrease in expression of anti-apoptotic protein BCL-xl was observed. These findings were aggravated by AOAA while using NaHS improved them. Conclusion: it seems that increasing bioavailability of H2S could have a protective role against CUS induced gastric lesions. The results suggest that this protection could be through anti-oxidant and anti-apoptotic effects.
https://besps.journals.ekb.eg/article_95284_8856c728f3363157fda2b3f671885eea.pdf
2020-06-01
1
15
10.21608/besps.2019.16189.1032
hydrogen sulfide (H2S)
chronic unpredictable stress (CUS)
Caspase 3
BCL-xl
mahmoud
el tohamy
dr.m.eltohamy@mans.edu.eg
1
department of medical physiology,faculty of medicine, Mansoura university, mansoura, Egypt
LEAD_AUTHOR
Mohammad
Ghalwash
dr_ghalwash@mans.edu.eg
2
department of medical physiology, faculty of medicine, mansoura university, mansoura, egypt
AUTHOR
Nisreen
Abo-Elmaaty
nisreenomar@hotmail.com
3
Department of Medical Physiology,Faculty of Medicine,Mansoura University, Mansoura, Egypt.
AUTHOR
refka
Messiha
refkakhalil@gmail.com
4
department of medical physiology, faculty of medicine , mansoura university, mansoura , egypt
AUTHOR
samah
Fouad
drsmahawad85@yahoo.com
5
Medical experimental research center (MERC), mansoura university, mansoura, egypt
AUTHOR
ORIGINAL_ARTICLE
Possible Effect of Abscisic Acid on Nitric Oxide in Isoproterenol-Induced Myocardial Infarction in Rats.
The aim of this study was to find out the possible role nitric oxide (NO) due to its nature as a second messenger in stress responses in the cardioprotective effect of the abscisic acid (ABA) in isoproterenol (ISO) -induced myocardial infarction in rats. Material and method: Thirty male rats distributed into: normal control group, myocardial infarction (MI) group, and MI group treated with ABA for one week. The cardiac effect of ABA on ISO-induced MI was evaluated by ECG, measuring mean arterial pressure, markers of heart injury in serum, levels of lipid peroxidation, and antioxidants in heart tissue, observing pathological changes of tissue, and indirect detection of NO was performed. Results: Compared with the MI group, it was found that ABA could ameliorated ISO-induced disturbances in the ECG pattern and in mean arterial pressure. Moreover, the concentration of creatine kinase MB (CK-MB), lactate dehydrogenase (LDH), troponinI (CTn-I), nitrite and nitrate in the serum all decreased in the MI+ABA group. The activities of catalase (CAT), and the content of reduced glutathione (GSH) in heart increased, on the contrary, the content of malondialdehyde (MDA) and iNOS was decreased. In addition, the histopathologic examination showed that pathological changes in the myocardium that was observed in the MI group, was reduced in the group treated with ABA. Conclusion: ABA protected myocardium against damage caused by an ISO through inhibition of lipid peroxidation, prevent the cytotoxic actions of the excessive NO release, and restored near normal architecture and function of myocardial.
https://besps.journals.ekb.eg/article_96868_660b7c3bed840a91d52bbab4a496bd8c.pdf
2020-06-01
17
30
10.21608/besps.2019.17609.1035
abscisic acid
Isoproterenol
myocardial infarction
Nitric oxide
Mona
Elhadidy
mona.gaber2012@yahoo.com
1
Department of Medical Physiology, Faculty of Medicine, Mansoura University, Egypt.
LEAD_AUTHOR
Mahmoud
Elalfy
dr_melalfym@mans.edu.eg
2
Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Mansoura University, Egypt.
AUTHOR
lashin
saad
lashin.saad@yahoo.com
3
Department of Medical Physiology, Faculty of Medicine, Mansoura University & Hours University, Egypt.
AUTHOR
ORIGINAL_ARTICLE
Nigella Sativa Seeds Afford Comparable Therapeutic Effect to that of Valsartan in Amelioration of Cardiovascular Complications in Dexamethasone-induced Hypertension Model in Adult Albino Rats
Aim: To identify the role of Valsartan and Nigella Sativa Seeds in amelioration of cardiovascular complications in dexamethasone-induced hypertension model in adult albino rats. Methods: Animals were randomized in into 4 groups. Group 1; a control negative group, without intervention. Group2; dexamethasone was administrated orally (0.3 mg/kg/day) for 1 month. Group 3: Nigella sativa ground seed in solution was administered orally via oral gavage (2.5 mg/kg/day) for 5 weeks. Group 4; Valsartan was administered orally (30 mg/kg/day) for 5 weeks. At day 1 baseline blood pressure measurement was done in all rats after proper conditioning for one week. Withdrawal of blood samples for spectral assay of NO and histopathological assessment of the heart and aorta were done. Results: there was an improvement in both the systolic and diastolic blood pressures in Nigella S. group and in Valsartan-treated group. NO assay showed increment in the aorta of the Nigella S. group only. The histopathological assessment has revealed a marked improvement in the Nigella S. group and Valsartan treated group with potentially better effect of Nigella S. Conclusion: Nigella S. has a better therapeutic potential in attenuating cardiovascular pathologies in rats treated with dexamethasone.
https://besps.journals.ekb.eg/article_95204_feca0eecb00ad22238c30387c3c00794.pdf
2020-06-01
30
43
10.21608/besps.2019.18819.1037
Nigella Sativa
Valsartan
Corticosteroids
Hypertension
Nitric oxide
Karima
El-Sayed
venose259@hotmail.com
1
Medical physiology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt
AUTHOR
Yasser
Awad
yasser_awad@agr.suez.edu.eg
2
Department of Agricultural Botany, Faculty of Agriculture, Suez Canal University, Ismailia
AUTHOR
Horeya
Erfan
dollar72009@hotmail.com
3
Department of Histology, Faculty of Medicine, Suez Canal University, Ismailia
AUTHOR
Shimaa
Yousof
drshimaay@gmail.com
4
Physiology department, Faculty of Medicine, Suez Canal University, Ismailia, Egypt
LEAD_AUTHOR
ORIGINAL_ARTICLE
THE PROTECTIVE EFFECT OF L- ARGININE AND ASCORBIC ACID ON DEXAMETHASONE INDUCED-HYPERTENSION IN RATS
Excess use of glucocorticoids either natural or synthetic has been associated with hypertension. Dexamethasone is a synthetic glucocorticoid with multiple uses. Recent studies demonstrated that dexamethasone induced hypertension is of endothelial dysfunction and decreased nitric oxide (NO) production. This work was designed to investigate the protective effect of L-arginine and ascorbic acid on dexamethasone induced hypertension in rats. Thirty adult male Albino rats were used in this study. Rats were equally divided into 5 groups: (G 1) Negative control group, (G 2): Positive hypertensive group, (G 3): L – arginine treated group, (G 4): Ascorbic acid treated group and (G 5): combined L-arginine and ascorbic acid treated group. L – arginine, ascorbic acid were given 2 weeks before induction of hypertension. Rats were followed up by measuring arterial blood pressure, Nitric oxide in urine and tissue homogenate, Total antioxidant capacity in serum and histopathology. Dexamethasone increased systolic (260.16 ± 28.9 mmHg) and diastolic blood pressure (149.8 ± 7.22 mmHg), decaresed NO in tissue (72.1 ± 5.2 μmol/ g (and urine (0.26 ± 0.04 μmol/ 24h(. Combination between L-arginine and ascorbic acid normalized systolic (118.6 ± 5.3 mmHg) and diastolic (79.5 ± 5.04 mmHg ) blood pressure and increased NO in aortic tissue (135.5 ±7.1μmol/ g) and urine (0.6 ± 0.08 μmol/ 24h) denoting that combination between L-arginine and ascorbic acid could effectively prevent dexamethasone induced hypertension
https://besps.journals.ekb.eg/article_95206_c424da5f77faeb9c13917a86b4ff4807.pdf
2020-06-01
44
53
10.21608/besps.2020.21575.1039
Nitric oxide
Oxidative Stress
vasodilatation
Mohamed
Abdo
mohamedabdo75@hotmail.com
1
Physiology department, Faculty of Medicine, Suez Canal University, Ismailia ,Egypt
LEAD_AUTHOR
Lobna
Nagy
rawdet_eljanna@yahoo.com
2
Physiology department, Faculty of Medicine, Suez Canal University, Ismailia
AUTHOR
Magda
Mohamed
magdamandor@gmail.com
3
Department of Medical Physiology, Faculty of Medicine, Suez Canal University
AUTHOR
ORIGINAL_ARTICLE
Maternal Metabolic Alterations in Monosodium Glutamate Fed Rats during Gestation and Lactation Period
Background: Monosodium glutamate (MSG), an extensively used food additive, is claimed to cause many health problems, its use is still under debate. Aim: to explore the metabolic and hypertensive effect of MSG dietary administration in pregnant and lactating female rats, and its possible underlying mechanism. Methods: 16 adult female albino Wister rats were divided into 2 groups, control group; were fed standard rat chow, and MSG group; were fed 2% MSG supplemented rat chow throughout gestation and lactation period. Oral glucose tolerance test (OGTT) and arterial blood pressure (ABP) were measured at mid and late gestation, mid and late lactation periods. At mid lactation period, a retroorbital samples were analyzed for fasting glucose, lipid profile; triglycerides (TGs), total cholesterol (TC) and low density lipoprotein-cholesterol (LDL-C), hormonal assays; insulin, glucagon, prolactin, corticosterone and ACTH, as well as pancreatic lipase, amylase and malondialdehyde (MDA). By the end of lactation, adipose tissue was assessed for glucose transporter-4 (GLUT-4) and hormone sensitive lipase (HSL) relative expression. Results: MSG rats showed elevated ABP during pregnancy and lactation, with impaired OGTT at mid and late lactation period. Fasting serum glucose, TGs, TC, LDL-C, glucagon, corticosterone, pancreatic lipase and amylase, MDA as well as adipose tissue HSL expression were significantly elevated, while, fasting serum insulin, ACTH and adipose tissue GLUT-4 expression were significantly declined in MSG fed rats as compared to control group. Conclusion: MSG feeding during pregnancy and lactation induced hypertension and metabolic alterations that could be due to pancreatic and adrenal affection secondary oxidative stress.
https://besps.journals.ekb.eg/article_95207_c18871c5b50b0db741bd280acce4eafc.pdf
2020-06-01
54
69
10.21608/besps.2020.21680.1040
MSG
Pregnancy
lactation
metabolism
pancreatic function
Doaa
Abou-Bakr
doaa1510@gmail.com
1
Physiology department, Faculty of medicine, Ain shams university, Cairo, Egypte
LEAD_AUTHOR
Rania
Mansour
dr_raniasalah@yahoo.com
2
Physiology department, Faculty of medicine, Ain shams university, Cairo, Egypt
AUTHOR
ORIGINAL_ARTICLE
Effect of Quercetin and Metformin on Glucose Transporter-4 Expression, Oxidative Stress, Inflammation Markers and Insulin Resistance in Type 2 Diabetes Mellitus
Background: Defects in glucose transporter-4 (GLUT-4) expression and function in the skeletal muscles and adipose tissue can be considered as the main cause of insulin resistance (IR) in type 2 diabetes mellitus (T2DM). Also, Oxidative stress and inflammation play a very important role in the development of IR and T2DM. Aim: Studying the effect of quercetin and metformin on GLUT-4 expression, oxidative stress, inflammation markers and IR in T2DM.Method: This study was carried out on 50 male Wistar rats which were divided into five groups; control, untreated diabetic rats, diabetic rats treated with metformin, diabetic rats treated with quercetin and diabetic rats treated with metformin and quercetin. Results: The use of metformin and quercetin separately produced significant decrease in plasma glucose, insulin, IL-6, TNF-α levels, HOMA-IR and TBARS level in skeletal muscle. Also, they caused significant elevation in the antioxidant enzyme activities in skeletal muscles with increased expression of GLUT-4 in the skeletal muscles and adipose tissue compared to the diabetic rats. Evidently, the quercetin effects were more significant than that of metformin on all the parameters except on HOMA-IR (similar significant improvement). Moreover, the combined use of quercetin and metformin produced highly significant improvement approaching control level in all the parameters than that observed by using either of them. Conclusion: The combined use of QC and MF improved hyperglycemia and IR by increasing the expression of GLUT-4 in skeletal muscles and adipose tissue together with the reversal of the oxidative stress and inflammatory states.
https://besps.journals.ekb.eg/article_95208_1042651769430855862e813585a6ae98.pdf
2020-06-01
70
85
10.21608/besps.2020.22519.1041
Glucose transporter-4
Quercetin
Metformin
Insulin Resistance
Type 2 diabetes mellitus
Magdi
Eldamarawi
magdidamarawi@med.tanta.edu.eg
1
Medical Physiology, Faculty of Medicine, Tanta University, Tanta, Egypt
LEAD_AUTHOR
Marwa
Abdelazeem
marwaabdelhaq@gmail.com
2
Pathology department, Faculty of Medicine, Tanta University, Tanta, Egypt
AUTHOR
ORIGINAL_ARTICLE
Functional and morphological consequences after atorvastatin intake in a rat model of intracerebral haemorrhage
Intracerebral haemorrhage (ICH) is a devastating disease for which there are currently no curative treatment options. Hypolipidemia has been recognized as a possible risk factor for ICH. Several studies have demonstrated that low cholesterol is a risk factor for ICH; others have reported that hypercholesterolemia is protective against ICH. The current study was designed to demonstrate the effect of low LDL levels on vascular endothelial cells integrity and subsequently on the development of ICH in rats. Material and methods: This study was carried on male Wistar albino rats divided into 2 groups: Group I: normal Sham-operated rats; Group II: ICH rats, were randomly subdivided into 3 subgroups (17 rat each): rats received 2% gum acacia daily orally; rats received atorvastatin 10 mg/kg daily alone or with L-arginine (143 mg/kg) orally for four weeks before induction of ICH and continued for one week after. Behavioural tests were performed 2, 24, and 48 h after ICH and 7 days later. Serum lipid profile was assessed. Brains were dissected and assessed macroscopically and microscopically for the extent of hematoma, brain water content and endothelin level in the brain homogenate were investigated. Immunoassaying for glial acidic fibrillary protein and Ki67 were performed. Results& Conclusion: Rats received atorvastatin and L-arginine showed a significant decrease in serum cholesterol and low-density lipoprotein (LDL) concentration. ICH led to marked impairment in motor functions. Atorvastatin and L-arginine intake before haemorrhage failed to impose any protection on the motor deficits with corresponding large hematoma size with disruption of nearby tissue.
https://besps.journals.ekb.eg/article_95209_eb88801df66c8dccb134c6d32126fce5.pdf
2020-06-01
86
102
10.21608/besps.2020.23202.1043
Intracerebral haemorrhage
statins
Endothelial cells
glial acidic fibrillary protein
Ki67
Noha
Badae
dr.nohabadi3@yahoo.com
1
Department of Medical Physiology-Alexandria Faculty of Medicine-Egypt
LEAD_AUTHOR
Noha
Zahran
nohamzahran@yahoo.com
2
Department of Histology and Cell biology- Alexandria Faculty of Medicine-Egypt
AUTHOR
Azza
Baraka
azzabarakamnh@gmail.com
3
Department of Clinical Pharmacology- Alexandria Faculty of Medicine- Egypt
AUTHOR
Rasha
Elshinety
rashaelshinety@yahoo.com
4
Department of Anatomy- Alexandria Faculty of Medicine- Egypt
AUTHOR
Abeer
Dief
abeeredief@gmail.com
5
Department of Medical Physiology-Alexandria Faculty of Medicine-Egypt
AUTHOR
ORIGINAL_ARTICLE
Potential role of transforming growth factor β1 and brain derived neurotrophic factor in Alzheimer and multi-infarct dementias.
Dementia is a progressive cognitive impairment of variable causes. It is considered a global health challenge worldwide as it represents a major comorbidity in the absence of specific treatment or cure. Neurotrophic factors like transforming growth factor β1 (TGFβ1) and brain derived neurotrophic factor (BDNF) have been emerged to be a corner stone in synaptic plasticity and memory. Our work aimed to recognize the potential role of TGFβ1and BDNF in cognitive impairment estimated by the psychometric tests of Alzheimer and multi-infarct dementias. Thirty demented patients divided into two groups (15 in each group) and 25 healthy matched controls were included. Diagnosis of dementia and estimation of psychometric features using modified mini mental state examination (3MS), memory assessment scale (MAS), and beck depression inventory scale were performed. Serum TGFβ1 and BDNF were also assessed in all groups. Psychometric tests revealed significant cognitive impairment in both groups compared to normal control. There was significant increase in the serum level of TGFβ1 and significant decrease in BDNF in both demented groups compared to control group. A significant positive correlation was found between the cognitive impairment and serum TGFβ1 in both demented groups. In conclusion, our findings suggested that serum TGFβ1 levels could reflect the severity of dementia regardless its cause, in addition, depletion of BDNF might be a possible mechanism of cognitive deterioration in dementia.
https://besps.journals.ekb.eg/article_95574_10e575bfc938dfb99d2e7a5b8b8805e3.pdf
2020-06-01
103
112
10.21608/besps.2020.23455.1044
Key words: Alzheimer dementia (AD)
Brain derived neurotrophic factor (BDNF)
Multi-infarct dementia (MID)
Transforming growth factor β1 (TGFβ1)
omyma
Ahmed
omyma_galal@hotmail.com
1
Department of physiology, Faculty of Medicine ,Assiut University, Assiut Egypt
AUTHOR
Eman
Khedr
emankhedr99@yahoo.com
2
Neurology and Psychiatry Department, Faculty of Medicine, Assiut University hospital.
AUTHOR
hanaa
mohammed
hanaa.dwak@yahoo.com
3
Physiology department, Faculty of Medicine, Assiut University.
LEAD_AUTHOR
Asmaa
Gomaa
asmaagom3a@yahoo.com
4
Medical Physiology Department,Faculty of Medicine, Assiut University.
AUTHOR
ORIGINAL_ARTICLE
Intermittent Calorie Restriction Ameliorates Behavioral Changes and Tau Hyperphosphorylation in Chronic Immobilization Stress in Rats.
Background: Chronic stress has been linked to mood and anxiety disorders and alteration of cognitive functions, such as memory. Intermittent calorie restriction (ICR) is a repeated mild stress that enhances the cell ability to combat more severe stress. Aim: Examination of the effects of ICR on rat behaviour and Tau hyperphosphorylation in chronic immobilization stress (CIS) rat model. Methods: 32 rats were divided into 4 equal groups: control, ICR (subjected to ICR protocol), CIS (subjected to CIS protocol), and CIS+ICR (subjected to both CIS and ICR protocols). After performing behavioural (open field and Barnes maze) tests, serum level of corticosterone, tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were determined, and hippocampal tissue level of phosphorylated Tau (phospho-Tau), noradrenaline, and serotonin were measured. Results: We found that, ICR significantly altered the stress-induced anxiety-like behavior and memory disturbances (P<0.001). Rats exposed to both ICR and CIS protocols had significantly lower levels of phospho-Tau, corticosterone, and TNF-α, and enhanced levels of noradrenaline and serotonin than those subjected to CIS alone (P<0.01). Conclusion: our results suggest that ICR protects against chronic stress-induced anxiety-like behaviour, memory disturbance and Tau hyperphosphorylation, possibly through inhibition of hypothalamic pituitary adrenal (HPA) axis, anti-inflammatory effects, and enhancement of noradrenaline and serotonin hormones.
https://besps.journals.ekb.eg/article_95377_00509ec8b03b60e436bd2f8178cd8c4f.pdf
2020-06-01
113
125
10.21608/besps.2020.23788.1045
calorie restriction
locomotor activity
Stress
tau proteins
Dalia
El Agamy
dalia.elagami@med.menofia.edu.eg
1
Physiology Department, Faculty of Medicine, Menoufia University, Egypt
LEAD_AUTHOR
Fatma
Ahmed
fatma1979isla@gmail.com
2
Clinical Pharmacology Department, Faculty of Medicine, Menoufia University, Egypt
AUTHOR
Safa
Elfiky
safariad55@gmail.com
3
Clinical Pharmacology Department, Faculty of Medicine, Menoufia University, Egypt
AUTHOR
ORIGINAL_ARTICLE
Effect of Electrical Microcurrent on Median Nerve Conduction Velocity and Mechanical Pain Threshold in the Median Nerve in a Randomized Single Blind Controlled Trial
Background: Microcurrent electrical stimulation (MES) is a promising line for treating a variety of conditions. Its outcome on the peripheral nerves remains vague. Objective: The purpose of this work was to assess the impact of MES on nerve conduction velocity (NCV) of the median nerve and pressure pain threshold in healthy people. Subjects and Methods: It was a randomized single-blind controlled trial that was conducted on sixty healthy students of the Faculty of Physical Therapy, Cairo University. Participants were assigned randomly into two groups: control and study groups; who were exposed to MES for 30 minutes using a frequency of 10 Hz, and intensity of 100 µA at the volar aspect of the non-dominant forearm. Median NCVs (motor and sensory) and pain pressure threshold were assessed before, immediately after and 30 minutes after the MES application. Results: Concerning the pain pressure threshold, there was a significant difference between both control and study groups favoring the study group (p-value < 0.05), and between pre and post measures of the sensory distal latency and sensory nerve conduction velocity (SNCV) in the study group (p-value < 0.05). While no significant results on median nerve motor parameters were recorded (p-value > 0.05). Conclusion: Within the limitation of this study, a single application of MES over the course of the median nerve in healthy subjects was effective in increasing pressure pain threshold, and sensory distal latency; and decreasing SNCV, So, upon these results MES could be promising in treating painful conditions.
https://besps.journals.ekb.eg/article_95210_7a4996e090063d6dc30c11da0f4afd48.pdf
2020-06-01
126
135
10.21608/besps.2020.25392.1046
Median nerve
Microcurrent electrical stimulation
Nerve conduction velocity
Pressure pain threshold
Randomized Single-Blind Controlled Trial
Fatma
Hassan
fatma.e.elsayed@kasralainy.edu.eg
1
Physiology department- Faculty of Medicine-Cairo University, Egypt
LEAD_AUTHOR
Salah Eldin
Elsayed
utcsalah@cu.edu.eg
2
Assistant professor at Basic Sciences Department- Faculty of Physical Therapy-Cairo
AUTHOR
Olfat
Ali
olfat_ib@yahoo.com
3
Assistant professor at Basic Sciences Department- Faculty of Physical Therapy-Cairo
AUTHOR
ORIGINAL_ARTICLE
Exogenous versus Endogenous Stem Cells Impacts on Knee Articular Cartilage Repair, Pain Sensation and Gait in a Rat Model of Osteoarthritis; Structural and Functional Assessment
Osteoarthritis (OA) is a joint disease with a very limited available curative option. Stem cells-based therapy revealed a promising regenerative capability in cartilage repair. We aimed to compare the sole and the combined effects of bone marrow derived mesenchymal stem cells (BM-MSCs) and the granulocyte colony stimulating factor (G-CSF) mobilized endogenous stem cells in articular cartilage repair, pain and gait improvement in monoiodoacetat (MIA)-induced rat model of osteoarthritis. OA was induced by a single intra articular injection 1 mg MIA. BM-MSCs-treated and the combined groups received a single intra-articular of injection of BM-MSCs. G-CSF-treated and the combined groups received subcutaneous injections of G-CSF. The articular cartilage repair, total leucocytic count, nociception behavior and gait parameters were assessed. The results revealed an increase in total leucocytic count on the 5th day after G-CSF injection and returned to normal on day 35. All treated groups showed comparable improvement in nociception behavior and gait parameters. Histopathological evaluation showed enhanced cartilage repair in the combined group compared to the two other treated groups. In conclusion The concomitant application of BM-MSCs and G-CSF have a promising comparable effect of exogenous and mobilized endogenous stem cells on pain, gait and the structural improvements.
https://besps.journals.ekb.eg/article_95205_80217e727221f5209bba90dd84776c63.pdf
2020-06-01
136
153
10.21608/besps.2020.19234.1038
articular cartilage
Bone marrow derived mesenchymal stem cells
Granulocyte colony stimulating factor
osteoarthritis
Heba
Sadek
hebaaa_2008@yahoo.com
1
Department of Medical Physiology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt
AUTHOR
Horeya
Korayem
dollar72009@hotmail.com
2
Department of Histology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt
AUTHOR
Shimaa
Yousof
drshimaay@gmail.com
3
Department of Medical Physiology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt
LEAD_AUTHOR
Dalia
Abd-Elhalim
dalia1431@yahoo.com
4
Department of Medical Physiology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt
AUTHOR
ORIGINAL_ARTICLE
Is there a possible impact of erythropoietin hormone on peripheral neuropathy going with acute renal damage in fully-grown male rats?
Background: erythropoietin hormone (EPO) with its great built erythropoietic impact, has recorded a lot of protective impacts on particular body organs and tissues. A standout amongst these is its renoprotective impact which is interceded through various mechanisms. Objective: as neuropathy had been reported frequently amongst patients with severe renal damage, this work had been made to record any possible benefits of EPO on the peripheral neuropathy that might go with acute renal failure (ARF). Methods: This study was carried out in the Faculty of Medicine, Cairo University, in January- 2019, on forty rats that had been randomly separated into four groups; Group I: control rats, Group II: ARF, Group III: ARF+EPO, and Group IV: EPO+ARF. Results: EPO injection had significantly reduced the levels of serum creatinine, blood urea nitrogen and (malondialdehyde and nerve growth factor within the nerve fibers). Also, 24-hours urine volume, glomerular filtration rate, nerve conduction velocity and amplitude besides the neural level of superoxide dismutase had been fundamentally improved. Conclusion: from this study, we had concluded that EPO intake had exerted protective impacts on peripheral neuropathy following ARF.
https://besps.journals.ekb.eg/article_95211_e4485e71d75a4e61a7eada6c2227fba6.pdf
2020-06-01
154
164
10.21608/besps.2020.25404.1047
erythropoietin
Nerve conduction velocity
Nerve Growth Factor
Neuropathy
Oxidative Stress
Fatma
Hassan
fatma.e.elsayed@kasralainy.edu.eg
1
Physiology department- Faculty of Medicine-Cairo University, Egypt
LEAD_AUTHOR
Dalia
Eid
daliaeid19740@gmail.com
2
Biochemistry Department, Faculty of Medicine, Ain Shams University, Egypt
AUTHOR