Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
37
1
2017
06
01
Circulating miRNA-146a and miRNA-146b-5p in psoriasis and in response to psoriasis therapy
1
17
EN
Naglaa
Ibrahim
Azab
Department of Medical Biochemistry, Faculty of Medicine, Benha University, Benha - Egypt.
naglaa.azab@fmed.bu.edu.eg
Aliaa
Daifalla
Department of Dermatology, Venereology and Andrology, Faculty of Medicine, Benha University, Benha– Egypt.
10.21608/besps.2017.8220
MiRNA-146a is overexpressed in psoriasis. MiRNA-146a and miRNA-146b have partially overlapping functions. We evaluated blood miRNA-146a/b expression by syber green real time-PCR in psoriasis and in response to mono-therapy using narrow-band ultraviolet B (NB-UVB) phototherapy or methotrexate (MTX), and evaluated if they are possible biomarkers for disease activity and for response to NB-UVB and MTX therapies.. MiRNA-146a/b expression significantly increased in psoriasis patients before therapy than in the controls. However, miRNA-146a expression showed significantly higher fold increase than miRNA-146b. Moreover, miRNA-146a/b expression levels before therapy were significantly higher in the MTX group than in the NB-UVB group, which coincided with the higher PASI score in the MTX group before therapy. In addition, miRNA-146a/b expression significantly decreased in response to both therapies. The fold decrease after MTX therapy relative to before MTX therapy was significantly higher than that after UVB therapy relative to before UVB therapy, which coincided with the significantly higher reduction effect of MTX on the PASI score. Only miRNA-146a significantly and positively correlated with PASI score. In conclusion, miRNA146a/b expression levels are increased in psoriasis and decreased in response to psoriasis therapy and may be used in predicting the response to NB-UVB therapy or methotrexate. Also, miRNA-146a may be used as a biomarker for disease activity.
miRNA-146a,miRNA-146b,Psoriasis,Narrow-band ultraviolet B phototherapy,Methotrexate
https://besps.journals.ekb.eg/article_8220.html
https://besps.journals.ekb.eg/article_8220_6ef27f7dd2b63a50e14947f802bae63f.pdf
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
37
1
2017
06
01
Study of thrombophilic gene polymorphisms in Egyptian patients with deep venous thrombosis using in situ hybridization technique.
18
35
EN
Naglaa
Ibrahim
Azab
Department of Medical Biochemistry, Faculty of Medicine, Benha University, Benha - Egypt.
naglaa.azab@fmed.bu.edu.eg
Rabab
Fawzy
Salim
Department of Medical Biochemistry, Faculty of Medicine, Benha University, Benha - Egypt.
Mohamed
Saeed
Darwish
Department of Cardiovascular medicine, Faculty of Medicine, Benha University, Benha - Egypt.
10.21608/besps.2017.8221
Up to 70% of thrombotic patients with no identifiable risk factors were termed idiopathic. Now, molecular diagnostics combined with existing laboratory techniques allow accurate classification of at least half of patients with inherited thrombotic disorders. However, the previously studied genetic variants explain only a fraction of all thromboembolic events, so the aim of the present study was to expand the genetic prevalence determination to include also more extensive thrombophilic gene polymorphisms in patients with DVT compared to healthy subjects in Egyptian population. This study was conducted on 75 patients with DVT and 45 age and sex matched healthy subjects as control group. The diagnosis of DVT was based on patient’s history, clinical findings, D-dimer test, and confirmed by Doppler ultrasonography. Both groups were assessed for thrombophilic gene polymorphisms using multiplex polymerase chain reaction and reverse-hybridization technique through CVD strip assay. It was found that FV Leiden G1691A (P=0.001), Factor V H1299R (P=0.02), MTHFR A1298C (P=0.02), β-fibrinogen−455 G/A (P=0.01), PAI-1 4G/5G (P=0.03) and ACE (P=0.03) polymorphisms were all significantly associated with an increased risk of DVT. Our data are of extreme importance in clinical practice for introducing the extended CVD panel into routine molecular diagnostic tests to allow management of thrombotic patients and screening, thromboprophylaxis and genetic counselling for high-risk individuals.
Venous Thromboembolism,Deep Venous Thrombosis,Genetic risk factors,Thrombophilic gene polymorphisms,Cardiovascular disease strip assay
https://besps.journals.ekb.eg/article_8221.html
https://besps.journals.ekb.eg/article_8221_3d30978cb8fb68ae22c9a1fe1ab57a6a.pdf
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
37
1
2017
06
01
Impact of chronic hepatitis C infection on diabetic patients with proteinuria
36
44
EN
Azza
M.
Abdu Allah
Department of Medical Biochemistry, Faculty of Medicine and National Liver Institute, Menoufia University, Egypt.
azza.abdallah@yahoo.com
Nesreen
G.
Elhelbawy
Department of Medical Biochemistry, Faculty of Medicine and National Liver Institute, Menoufia University, Egypt.
Ayman
Alsebaey
Hepatology Departments Faculty of Medicine and National Liver Institute, Menoufia University, Egypt.
10.21608/besps.2017.8223
Background: Hepatitis C (HCV) and diabetes mellitus (DM) are major health burden with various systemic complications. Both affect the kidney with increasing the incidence of progression to end stage renal disease. Aim: Usefulness of tumor necrosis factor (TNF), high-sensitivity CRP (hs-CRP) and interleukin-18 (IL-18) as differentiating markers of proteinuria in HCV diabetic patients. Methods: Eighteen as control and 72 patients with DM were enrolled. Diabetic patients were divided into 4 equal groups; diabetic patients with microalbuminuria negative for HCV (n=18), positive for HCV (n=18), diabetic patients with macro-albuminuria negative for HCV (n=18), positive for HCV (n=18). TNF, hs-CRP and IL-18 were measured in all groups. Results: TNF was not useful for distinguishing microalbuminuria (174.61 ±64.89 vs. 229.33 ±101.31 pg/mL; p=0.093) or macroalbuminuria (69.78 ±22.34 vs. 78.00 ±20.57 pg/mL; p=0.259) in HCV diabetic patients. The hs-CRP had a paradox relationship where higher levels were found in HCV diabetic patients with microalbuminuria (6.42 ±0.76 vs. 4.43 ±0.70 mg/L; p=0.001) unlike those with macroalbuminuria (5.25 ±0.69 vs. 6.72 ±1.56 mg/L; p=0.003). IL-18 increased in microalbuminuria (619.72 ±72.53vs 148.54 ±5.69 mg/L; p=0.001) or macro-albuminuria (609.22 ±52.05 vs. 352.44 ±12.33 pg/mL; p=0.001) in HCV diabetic patients. Conclusion: IL-18 is a promising.
HCV,DM,albuminuria
https://besps.journals.ekb.eg/article_8223.html
https://besps.journals.ekb.eg/article_8223_d7cf0fe9280403c9f74685b128e08fb3.pdf
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
37
1
2017
06
01
Effects of Caffeine Intake on Oxido/Inflammatory Axis in Rat Model of Non-Alcoholic Fatty Liver Disease
45
58
EN
Amal
Baalash
Department of Medical Biochemistry, Faculty of Medicine, Tanta University, Egypt.
Noha
M.
Shafik
Department of Medical Biochemistry, Faculty of Medicine, Tanta University, Egypt.
Abeer
A.
Abo Zeid
Department of Physiology, Faculty of Medicine, Tanta University, Egypt.
Abla
M.
Ebeid
Department of Clinical Pharmacy, Faculty of Pharmacy, Al-Delta University, Gamasa, Egypt.
10.21608/besps.2017.8225
The precise mechanism of caffeine modulating effects on NAFLD is completely unknown. So, the aim of this study was to evaluate the modulating effects of caffeine on some biochemical markers in obesity induced NAFLD. Forty male albino rats were divided into four groups of 10 rats each. The rats of group 1 served as normal control. Group 2 received control diet and caffeine in a concentration of 1g/L orally. Group 3 received a high fat diet (HFD). Group 4 received high fat diet and was given caffeine just like group 2. After 20 weeks, serum and liver tissues were obtained from the sacrificed rats. Total lipid profile, blood glucose levels, liver triacylglycerols (TAGs) and serum activity of alanine aminotransferase (ALT) were estimated. Tissue 4-hydroxy-2-nonenal (4-HNE) as a marker for oxidative stress, calgranulin S100 A8, receptor for advanced glycation end products (RAGE) and toll like receptor-4 (TLR4) were immunoassayed. Plasma caffeine levels were estimated by ultra performance liquid chromatography. NAFLD was confirmed by histopathological results as well as increased serum activity of ALT, marked dyslipidemia and high blood glucose levels. NAFLD led to elevations in liver TAGs, 4 –HNE, calgranulin S100 A8, RAGE and TLR4. Improvement in all assessed parameters except TLR4, was observed in NAFLD rat group treated with caffeine. Caffeine had a modulatory action on NAFLD which may be through improving the antioxidant status in hepatic tissue and/or suppressing some inflammatory cascades. It is hoped that these findings would assist in development of NAFLD management strategies that may use caffeine as a potential drug for treatment.
Obesity ,non alcoholic fatty liver disease (NAFLD),,caffeine ,4-hydroxy-2-nonenal (4-HNE) ,calgranulin S100 A8 ,receptor for advanced glycation end products (RAGE) ,toll like receptor,4(TLR4)
https://besps.journals.ekb.eg/article_8225.html
https://besps.journals.ekb.eg/article_8225_9da7660c75c920a3621254bf48bcfd44.pdf
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
37
1
2017
06
01
Value of Serum miRNA 146a as Biomarker for Early Diagnosis of Neonatal Sepsis
59
68
EN
Inas
A.
Ahmed
Department of Medical Biochemistry, Molecular Biology and Biotechnology Unit, Faculty of Medicine, Benha University, Al Qalyubia, Egypt
inas.ahmed@fmed.bu.edu.eg
Amal
A.
Elfadle
Department of Medical Biochemistry,Faculty of Medicine, Benha University,Egypt.
Ghada
Saad
Abdelmotaleb
Department of Pediatrics, Faculty of Medicine, Benha University,Egypt.
10.21608/besps.2017.8226
Neonatal sepsis (NS) is regarded as one of the most common causes of morbidity and mortality among neonates worldwide. However, the magnitude of the problem is higher in low income countries, in spite of recent improvement of neonatal health care units. Accurate and early diagnosis of NS is the corner stone toward better therapeutic approach and safe outcome. C-reactive protein is currently used however it has been reported to have a low specificity and to rise in blood of cases with a delay of 24 hours than other biomarkers like inerleukin-16 or procalcitonin. Therefore, identification of new biomarkers with high specificity and sensitivity is extremely needed. The current study aimed to detect changes in miR-146a serum expression levels among NS cases, neonates at high risk to develop sepsis and healthy control neonates and to assess the value of serum miR-146a as early indicator of NS. qPCR was used for detection of miR-146a serum expression among different study groups. Serum miR-146a showed differential expression of statistical significance within study groups, with the highest level in NS group and the lowest in healthy controls. Moreover, serum miR-146a showed a significant positive correlation with the CRP titer in NS group. Receiver operating characteristic (ROC) curve analysis revealed that best cut off value for miR-146a was at 0.539 relative units, with a sensitivity of 91.7%, specificity of 100% and 95.7% accuracy. As regard to CRP, the best ROC curve was at 18 mg/dL with 100% sensitivity, 86.4% specificity and 93.5% accuracy. Combination of miR-146a and CRP increased specificity, sensitivity and accuracy to 100%. Furthermore, in multivariate logistic regression analysis, only CRP and miR-146a were considered as independent risk factor for prediction of sepsis within high risk neonates. In conclusion, serum miR-146a may be a promising predictor for sepsis within the high risk neonates. However, large-scale prospective studies are required to confirm our findings.
Neonatal sepsis,miRNAs,Systemic inflammatory response syndrome
https://besps.journals.ekb.eg/article_8226.html
https://besps.journals.ekb.eg/article_8226_f78975f48d593405f03ff712843044e8.pdf
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
37
1
2017
06
01
The relationship between the IL-1β 31gene polymorphism and HCC in Egyptian patients
69
79
EN
Abdelnaser
Badawy
Medical Biochemistry Department, Faculty of Medicine, Mansoura University Egypt.
Rehan
Monir
Medical Biochemistry Department, Faculty of Medicine, Mansoura University Egypt.
Heba
Kamal
Department of Medical Biochemistry, Mansoura Faculty of Medicine, Mansoura University, Egypt.
kamalheba@hotmail.com
Nader
Elmalky
Internal medicine Department, Faculty of Medicine, Mansoura University, Egypt.
Amr
El-rabat
Internal medicine Department, Faculty of Medicine, Mansoura University, Egypt.
Mahmoud
Abdelghafar
Oncology Center, Surgery Department, Mansoura University, Egypt.
10.21608/besps.2017.8227
Background: Hepatocellular carcinoma (HCC) is one of the most common cancers worldwide and has an increasing incidence in Egypt. Hepatitis virus (HCV and HBV) are the major risk factors for developing HCC. IL-1β, a proinflammatory cytokine has been suggested to affect the hepatic carcinogenesis. Aim of the work: Was to evaluate the role of IL-1β polymorphism in the occurrence of HCC on top of viral versus none-viral etiology. Patients and Methods: The study included 178 patients with HCC (74 with HCV, 48 with HBV, and 56 without hepatitis virus) and 90 healthy volunteers represented the control group. The polymorphism in IL-1β –31 gene was investigated by polymerase chain reaction and restriction fragment length polymorphism. Quantitative determination of IL-1β serum level was performed using ELISA technique. Results: The frequency of TT genotype was higher in HCC patients with HCV when compared to HCC patients without viral hepatitis, and the control group (43.2%, 25% and 26.7%, respectively). We observed that the dominant model (TT and CT genotypes) were associated with increased HCC risk in HCV or HBV patients compared to the control group with odd ratio and 95%CI of 2.64 (1.3-5.3) and 2.77 (1.2-6.2) respectively. In addition, the T allele was more frequent in HCC patients with HCV or HBV when compared to none viral HCC and the control group (60.8%, 56.2%, 42.9 and 42.2%, respectively). Serum IL-1β level was elevated in all HCC groups as compared to the control group (p<0.05). Serum level of IL-1β was considerably increased in individuals with TT genotype or T allele as compared to other genotypes and allele (p<0.0001). Conclusion: IL-1β TT genotype and T allele which are associated with high blood IL-1β level may increase the risk of HCC in patients with chronic viral hepatitis.
IL-1 β,Polymorphism,viral hepatitis,Hepatocellular carcinoma
https://besps.journals.ekb.eg/article_8227.html
https://besps.journals.ekb.eg/article_8227_3368984ed38d9312f528e0bd4708a276.pdf
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
37
1
2017
06
01
Nitric oxide/Asymmetric Dimethylarginine Axis may Interplay With Some Sex Hormones for Pathogenesis and Severity of Pre-eclampsia: Study controlled by Uterine Arterial Doppler.
80
91
EN
Adel
A.
Al-kholy
Department of Medical Biochemistry, Faculty of Medicine, Benha University, Egypt.
adeladel59@yahoo.com
Manal
Hassan
Department of Medical Biochemistry, Faculty of Applied Medical Sciences, 6 October University, Egypt.
Maha
M.
Serag
Department of Clinical Pathology, Faculty of Medicine, Tanta University, Egypt.
Hesham
M.
Abou Ragab
Department of Obstetrics & Gynecology, Faculty of Medicine, Benha University, Egypt.
10.21608/besps.2017.8229
Objectives: To evaluate relations of asymmetric dimethylarginine (ADMA) and nitric oxide (NO) with estrogen (E2) and progesterone (Pg) and its role in pathogenesis and severity of pre-eclampsia (PE). Patients & Methods: At 12th week gestational age (GA) constitutional data, systolic and diastolic blood pressures (SBP and DBP), uterine artery pulsatility index (UAPI) and serum NO, ADMA, E2 and Pg levels were determined for primigravida attending the antenatal care unit. During pregnancy, women who developed PE were categorized as early or late and mild or severe according to ACOG Practice Bulletin of PE guidelines. Studied parameters were evaluated statistically as early predictors for development and severity of PE. Results: Ninety pregnant women developed PE; 39 early and 51 late. Only 13 women had severe and 77 women had mild PE. At 12th week GA, UAPI and serum ADMA levels were significantly higher with significantly lower E2 and NO levels in PE women than women free of PE and in early than in late PE. High SBP at time of PE diagnosis showed positive significant correlation with body mass index (BMI) and serum ADMA levels but showed negative significant correlation with serum NO and E2 levels. Serum ADMA levels showed positive significant correlation with BMI, but negative significant correlation with serum E2 levels. High UAPI, serum ADMA and high BMI were significant early predictors for PE development and severity. Conclusion: Disturbed maternal serum ADMA/NO axis and low E2 serum levels may underlie PE development. High BMI and low E2 level may have a role in PE pathogenesis through induction of high serum ADMA levels. High UAPI and serum ADMA at 12th week GA could be used as early predictors of PE especially early-onset and severe PE.
Pre-eclampsia,pathogenesis,severity,Asymmetric dimethylarginine,Sex hormones,Uterine arterial Doppler
https://besps.journals.ekb.eg/article_8229.html
https://besps.journals.ekb.eg/article_8229_0580523b81aa44c4db5000ca1b2bbfb2.pdf