Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
32
1
2012
01
01
Epigenetic Silencing of WNT Inhibitory Proteins; SFRP1 and DKK3 in Acute Leukemia
1
14
EN
Ahmed
Shams
Department of Clinical Pathology, Faculty of Medicine, Cairo University
Dalia
GA
Department of Clinical Pathology, Faculty of Medicine, Cairo University
Shaimaa
S
Department of Clinical Pathology, Helwan Hospital.
Omar
Tolba
Cairo University Specialized
Pediatric Hospital, Pediatrics Department, Faculty of Medicine, Cairo University.
10.21608/besps.2012.35461
Background: Over-activation of Wnt (derived from names of two genes; Drosophila<br />Wingless and mouse Int-1) pathway is incriminated in leukemogenesis. Functional<br />loss of Wnt antagonists; DKK (Dikkopf) and SFRPs (secreted frizzled-related<br />protein), can contribute to Wnt hyper-activation. Silencing of Wnt antagonists by<br />hypermethylation is reported in human malignancies as well as in hematopoietic<br />malignancies. Our aim was to estimate the frequency and the possible impact of<br />hypermethylation of the SFRP1 and DKK3 in acute leukemia. Methods: We evaluated<br />SFRP1 and DKK3 methylation status using methylation specific polymerase chain<br />reaction (MS-PCR) in 50 acute myeloid leukemia (AML) and 30 B-acute<br />lymphoblastic leukemia (B-ALL) patients and 20 age and sex matched controls.<br />Results: The frequency of methylation in B-ALL patients was 40% for SFRP1, 40%<br />for DKK-3, in AML patients; the frequency was 44% for SFRP1, 36% for DKK-3. All<br />the control subjects had no aberrant methylation in either SFRP1 or DKK-3. B-ALL<br />and AML groups showed no statistical significant difference in the frequency of<br />SFRP1 or DKK-3 methylation. B-ALL patients with M-SFRP1 had a significantly<br />higher mean platelets count and a lower mean age compared to B-ALL patients with<br />UM-SFRP1, no other significant clinical or hematological difference was<br />encountered between patients with M-SFRP1 and UM-SFRP1 or between M-DKK3<br />and UM-DKK3 patients in the ALL or the AML group. Different B-ALL and AML<br />prognostic cytogenetic groups showed nearby frequency of SFRP1 and DKK3<br />methylation. Conclusion: SFRP1 and DKK3 methylation is frequent in acute<br />leukemia. Treatment with demethylating agents may reverse the overactivated Wnt<br />signaling in patients with methylated phenotype.
SFRP1,DKK3,methylation,AML,B-ALL,MS-PCR
https://besps.journals.ekb.eg/article_35461.html
https://besps.journals.ekb.eg/article_35461_c5ef75b2782feb855586e2cd048d3054.pdf
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
32
1
2012
06
01
Biochemical Effects of Passive Maternal Smoking as Measured by Serum Cotinine on Birth Weight, Oxidative Stress, Some Toxic Metals and Trace Elements
15
32
EN
Amany
Osama
Biochemistry Department, Faculty of Medicine, Assiut University
Nagwa
Ahmed
Biochemistry Department, Faculty of Medicine, Sohag University
Mervat
Khamis
Gynecology and Obstetrics Department,
Faculty of Nursing, Assiut University
10.21608/besps.2012.35474
Prenatal exposure to passive smoking has consequences both in childhood and in<br />adulthood. Cigarette smoking during pregnancy, preoxidant/antioxidant imbalance<br />might have pathomorphological and pathophysiological effect on fetus. Therefore the<br />aim of the present study was to estimate the effect of tobacco smoking during<br />pregnancy on activity of superoxide dismutase, and the levels of lipid peroxidation<br />marker, lead, cadmium, copper and zinc. The subjects of the study consisted of sixty<br />three parturient mothers and their neonates. Urine and serum samples were collected<br />from mothers and their neonates. Our results reveled that there was a significant<br />positive correlation between cotinine levels in meconium and both maternal urinary<br />and serum cotinine levels. It could be concluded that preventing and reducing<br />passive maternal smoking during pregnancy might have a beneficial impact on infant<br />size at birth.
https://besps.journals.ekb.eg/article_35474.html
https://besps.journals.ekb.eg/article_35474_7e046b32fb75089eadecbc75729a733a.pdf
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
32
1
2012
06
01
Heat-Shock Protein Expression and Oxidative Stress in Male Infertility
33
46
EN
Adel
Zalata
Medical Biochemistry, Faculty of Medicine, Mansoura University.
Nariman
Badr El-din
Zoology Dept.,
Faculty of Science - Mansoura University
Hussein
Abdalla
Medical Biochemistry, Faculty of Medicine, Mansoura University
Mahmoud
El-shahat
Zoology Dept.,
Faculty of Science - Mansoura University
10.21608/besps.2012.35482
In infertile men, it has been demonstrated that heat shock protein (HspA2) is<br />expressed in spermatogonia parallel the loss of spermatogenic function. Low level of<br />HspA2 expression in spermatogonia might lead to an altered level of protection,<br />which in turn could be involved in low spermatogenic efficiency. Aim: The present<br />study aimed to investigate the relationship between expression of heat shock protein<br />(HspA2) in ejaculated human sperm and oxidative stress in male infertility. Patients<br />& Methods: This study included 96 men attending the Andrology Outpatient Clinic,<br />Mansoura University Hospital. The semen samples obtained from men were grouped<br />according to WHO criteria into: Normozoospermia (N) was used as control group<br />(n=24), Asthenozoospermia (A) (n= 21), Astheno-Teratozoospermia (AT) (n=23) and<br />Oligo-Astheno-Teratozoospermia (OAT) (n=28). Computer assisted semen analysis<br />(Autosperm), hypo-osmotic swelling (HOS) test and acrosin activity of spermatozoa<br />by gelatinolysis test were performed. Also, malondialdehyde (MDA)/spermatozoa and<br />total antioxidant capacity (TAC) were assessed in seminal plasma. Expression level of<br />HspA2 mRNA of spermatozoa was determined by RT-PCR and DNA fragmentation<br />was detected by agarose gel electrophoresis. Result: The current study showed that,<br />percentage of DNA fragmentation was significantly increased in OAT group<br />compared to control group (N). Also, the present study showed significantly negative<br />correlation between MDA/spermatozoa with sperm concentration, grade A motility<br />grade A+B motility, velocity, linear velocity linearity index, normal morphology,<br />acrosin activity index, HOS test TAC and HspA2 expression. HspA2 expression and<br />TAC level showed significantly positive correlation with sperm concentration, grade<br />A motility, grade A+B motility, velocity, linear velocity, linearity index, normal<br />morphology, acrosin activity index, HOS test and HspA2 expression. Conclusion:<br />From results of the current study, it could be concluded that HspA2 gene expression<br />in ejaculated sperm from infertile might be associated with spermatogenic and/or<br />spermiogenic dysfunction involved in the pathogenesis of some cases of male<br />infertility.
https://besps.journals.ekb.eg/article_35482.html
https://besps.journals.ekb.eg/article_35482_eec401a1ffd356e99dee7dac1df81f93.pdf
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
32
1
2012
06
01
T cell receptor Zeta chain (TCRζ) expression in patients with idiopathic thrombocytopenic purpura
47
58
EN
Dalia
GA
Department of Clinical Pathology,
Faculty of Medicine, Cairo University
10.21608/besps.2012.35484
Background: T cell receptor zeta chain (TCRζ) plays a critical role in signal<br />transduction via TCR. Defective signaling through TCRζ-CD3 components may<br />disrupt peripheral T cell tolerance to autoantigen, giving rise to a variety of<br />autoimmune diseases. The aim of this work was to evaluate TCRζ chain gene<br />expression in patients with ITP, thereby to understand the functioning status of T cells<br />and its possible contribution to disease outcome. Methods: SYBR Green based Realtime<br />relative quantitative PCR (qRT-PCR) was used to evaluate TCRζ gene<br />expression in 49 ITP patients and 35 age- and sex-matched control subjects. Results:<br />Patients in acute phase of ITP had a significantly lower TCRζ gene expression<br />compared to those in chronic phase and those of the control group; on the other hand<br />TCRζ gene expression was significantly lower in chronic ITP patients than in<br />controls. Patients with active ITP had a significantly lower TCRζ gene expression<br />compared to those in remission and those of control group (P < 0.001). TCRζ gene<br />expression was significantly lower in ITP patients in remission state in comparison to<br />the control group. Treated ITP patients had a significantly higher TCRζ gene<br />expression compared to untreated patients, however TCRζ gene expression in the<br />treated group was still significantly lower compared to controls (P<0.001). Complete<br />responders had a significantly higher TCRζ gene expression compared to non<br />responders (P<0.001), while no significant difference was found between nonresponders<br />and partial responders as regards TCRζ gene expression (P>0.05). The<br />level of TCRζ gene expression in complete responders was still significantly lower<br />compared to controls (P<0.001). Sequential analyses for TCRζ gene expression in 6<br />patients with stable disease revealed no significant difference in median TCRζ gene<br />expression values in the 3 consequents analyses (P>0.05). TCRζ gene expression<br />showed no significant correlation with any of the clinical or hematological data.<br />Conclusion: From these data, it could be suggested that downregulation of TCRζ<br />may represent intrinsic defects of potential pathogenetic significance for ITP.<br />Therapy targeted to normalization of TCRζ expression may represent a new strategy<br />for treatment of ITP patients after being validated in clinical trials.
TCRζ,qRT-PCR,ITP
https://besps.journals.ekb.eg/article_35484.html
https://besps.journals.ekb.eg/article_35484_eda70a60248f16815ecda2456ee67edd.pdf
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
32
1
2012
06
01
Growth Hormone Releasing Hexapeptide-6 (GHRP-6): Possible Protective Role against Experimentally-Induced Osteoporosis in Female Albino Rats
77
92
EN
Ibrahim
Ibrahim
Department of Physiology, Faculty of Medicine, Minia University
Walaa
Nazmy
Department of Physiology, Faculty of Medicine, Minia University
Adel
Saad
Department of Physiology, Faculty of Medicine, Minia University
El-Shymaa
Abdel-Hakeem
Department of Physiology, Faculty of Medicine, Minia University
10.21608/besps.2012.35506
Postmenopausal osteoporosis is by far the most common cause of age related bone<br />loss. Growth hormone (GH) is not only important for linear body growth during<br />childhood, but it is also a major determinant of adult bone mass. GH secretion<br />diminishes with aging. Therefore, there might be a causal link between the agerelated<br />decline in GH secretion and bone loss after menopause. So, the present study<br />was designed to investigate the effect of growth hormone releasing hexapeptide<br />(GHRP-6), a synthetic GH secretagogue, on bone loss in ovariectomized albino rats<br />and to compare the results with those of estrogen replacement therapy as a strategy<br />for treatment in such condition. All rats (except the control rats) were subjected to<br />bilateral ovariectomy and were divided into four groups (10 rats each): sham<br />operated control, ovariectomized (OVX), OVX+ estrogen supplemented and<br />OVX+GHRP-6 treated groups. Rats were administrated their treatments<br />subcutaneously daily for 6 weeks. In the present study, GHRP-6 was equally powerful<br />as estrogen in preventing OVX-induced bone loss. Even more, it caused a +ve shift<br />between bone resorption and bone formation for the benefit of bone formation as<br />evidenced by the higher serum levels of alkaline phosphatase (a marker of bone<br />formation) without any significant change in acid phosphatase level (a marker of<br />bone resorption). In conclusion, GHRP-6 prevents ovariectomy-induced bone loss in<br />albino rats mainly via preservation of bone tissue and increasing bone formation.<br />Hence, GHRP-6 could be of value for postmenopausal osteoporotic women who<br />cannot tolerate estrogens or for whom estrogens are contraindicated.
GHRP-6,Ovariectomy,Osteoporosis,Estrogen,Rats
https://besps.journals.ekb.eg/article_35506.html
https://besps.journals.ekb.eg/article_35506_a58d6f995cfb6631de9503318bd1df19.pdf
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
32
1
2012
06
01
Biochemical and Hematological Effects of Food Flavoring Furfural in Male Albino Rats
93
110
EN
Tarek
Ali
Physiology Department, Faculty of Medicine,Beni-Suef University.
Osama
Ahmed
Physiology Division, Zoology Department, Faculty of Science,
Beni-Suef University
Rasha
Ahmed
Histology division, Zoology Department, Faculty of Science,
Beni-Suef University
10.21608/besps.2012.35529
Objectives: The present study aims to assess the effect of furfural, an aldehyde<br />commonly used as food flavor in many foods, on the hematological and biochemical<br />aspects in male albino rat. Materials and methods: The experimental animals used in<br />the study were divided into four groups. The control group was given a daily volume<br />of the vehicle dimethyl sulfoxide (DMSO) whereas the other three groups were orally<br />administered furfural for 8 weeks in a dose of 3.18, 6.36 and 12.72 mg/kg body<br />weight respectively. Blood samples were obtained for complete blood count (CBC),<br />liver and kidney function tests. Serum α-fetoprotein (AFP) and carbohydrate antigen<br />(CA19.9 were also measured. Results: Obtained results revealed a decrease in red<br />blood corpuscles (RBCs) count, packed cell volume (PCV) and hemoglobin content<br />and an increase in mean cell volume (MCV) and mean cell hemoglobin content<br />(MCH) in rats received furfural in a dose dependent manner. The white blood cells<br />(WBC) count was decreased specially esinophils percentage was depleted in the low<br />and medium doses groups. Furfural elevated serum liver enzymes activities and total<br />bilirubin concentration. The serum total proteins and albumin levels were increased<br />in medium and high doses groups, while globulin level, was elevated only in low dose<br />group. Furfural elevated serum urea level but did not affect serum AFP and CA19.9<br />levels in all groups. Conclusions: In conclusion, furfural administration to rats<br />induces a sort of macrocytic anemia, leucopenia, esinopenia and elevated liver<br />enzymes and urea in rats in a dose related manner.
https://besps.journals.ekb.eg/article_35529.html
https://besps.journals.ekb.eg/article_35529_2abb8de43890e650280317c67f48cbba.pdf
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
32
1
2012
06
01
Study of the Relation between Serum Total Homocysteine and Methylenetetrahydrofolate Reductase Gene Polymorphism In Renal Transplant Recipients
111
123
EN
Hala
El-Wakil
Department of Internal Medicine, Faculty of Medicine,
Alexandria University, Egypt
Iman
Diab
Department of Medical
Biochemistry, Faculty of Medicine,
Alexandria University, Egypt
Gihan
Sharara
Department of Medical
Biochemistry, Faculty of Medicine,
Alexandria University, Egypt
Sahar
Azab
Department of Cardiology, Faculty of Medicine,
Alexandria University, Egypt
Samer
Zahran
Department of Biochemistry, Faculty of
Pharmacy, Pharos University
10.21608/besps.2012.35539
The main cause of reduced long-term graft survival is chronic allograft injury.<br />Cardiovascular risk factors such as hyperhomocysteinemia seem to play an important<br />role. As atherosclerotic lesions in chronic allograft injury may be due to<br />hyperhomocysteinemia, we examined the hypothesis that the C677T variant of the<br />methylenetetrahydrofolate reductase (MTHFR) gene, which is linked to elevated<br />plasma homocysteine levels in patients with renal failure, determines renal allograft<br />dysfunction. Endothelial dysfunction probably has a role in this process. The aim of<br />the present work was to study the influence of the C677T MTHFR gene polymorphism<br />on plasma levels of homocysteine and folate in renal graft recipients, and their impact<br />on chronic graft dysfunction, as well as studying the relation between chronic<br />allograft injury and endothelial dysfunction by estimating von Willebrand factor<br />(vWF) and measuring endothelial dependent dilatation of the brachial artery (EDD).<br />The subjects included in this study were 32 renal allograft recipients (Group I) and<br />30 normal subjects as a control group (Group II). MTHFR genotype was determined<br />by PCR, subsequently the patients were further classified into three subgroups<br />according to the MTHFR genotypes: Group I (a): 6 allograft recipients with<br />homozygous- TT type. Group I (b): 8 allograft recipients with heterozygous- CT type.<br />Group I (c): 18 allograft recipients with wild- CC type. Estimation of total plasma<br />homocysteine concentration, plasma folic acid, plasma and von Willebrand factor<br />(vWF) were determined. Vascular responses of the brachi al artery were performed by<br />high resolution ultrasound imaging. This study showed significantly higher levels of<br />both homocysteine and von Willebrand factor (vWF) were found in renal allograft<br />recipients as compared to the control group. A negative correlation was found<br />between homocysteine levels and creatinine clearance suggesting<br />hyperhomocysteinemia contributes to the renal allograft dysfunction. No significant<br />difference was found as regards the plasma folic acid levels between the patients and<br />controls. Allograft recipients with MTHFR homozygous-TT type showed significantly<br />higher levels of homocysteine and vWF as compared to allograft recipients with<br />heterozygous-CT type and those with wild- CC type. Also allograft recipients with<br />homozygous- TT type showed lower levels of plasma folic acid and creatinine
clearance as compared to the other two subgroups. Lower endothelial dependent<br />dilatation of the brachial artery (EDD) was observed in renal allograft recipients as<br />compared to the control group. The EDD was significantly less in allograft recipients<br />with MTHFR homozygous- TT type than those with MTHFR heterozygous- CT type or<br />wild- CC type. CONCLUSION: The present study supports the hypothesis that the<br />C677T variant of the MTHFR gene is an important determinant of renal-transplant<br />survival, and that certain genotypes of MTHFR gene are associated with chronic<br />allograft injury. Hyperhomocysteinemia, elevated vWF, lower folic acid levels and<br />endothelial dysfunction together with certain genotypes of MTHFR gene increases the<br />risk of development of chronic allograft injury in renal transplant patients.
renal transplant,MTHF gene polymorphism,Homocysteine,Folic acid,Von Willebrand Factor,endothelial dysfunction
https://besps.journals.ekb.eg/article_35539.html
https://besps.journals.ekb.eg/article_35539_c502ed3a33aa371eba1793e87ca8d583.pdf
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
32
1
2012
06
01
Association of Il-6 and Il-10 Genes Polymorphisms with Breast Cancer in Egyptian Women
125
140
EN
Abdelnaser
Badawy
Medical Biochemistry Department, Faculty of Medicine, Mansoura University, Egypt
Hussein
Abdalla
Medical Biochemistry Department, Faculty of Medicine, Mansoura University, Egypt
Sabry
Mahmoud
General Surgery Department, Faculty of Medicine, Mansoura University, Egypt
Saad
Elshafei
Histology Department, Faculty of Medicine, Mansoura University, Egypt
Dina
Eltantawy
Pathology Department, Faculty of Medicine, Mansoura University, Egypt
10.21608/besps.2012.35550
Cytokines are factors that are known to have both tumor-promoting and inhibitory<br />effects on breast cancer growth depending on their relative concentrations and the<br />presence of other modulating factors. Genetic polymorphisms of cytokine-encoding<br />genes are known to predispose to malignant disease. Interleukins -10 and -6 are<br />crucially involved in breast carcinogenesis. The aim of this study is to evaluate the<br />role of IL-6 and IL-10 genes polymorphisms in the pathogenesis of breast cancer in<br />a case – control study of 80 females with breast cancer and 80 healthy females as<br />controls. Patients and controls underwent a clinical examination and finally, one ml<br />blood samples were withdrawn from all cases into tubes containing EDTA as an<br />anticoagulant for analysis of IL-6 and IL-10 genotypes. There were significant<br />association between C allele of the G−174 C promoter single nucleotide<br />polymorphism (SNP) in the IL-6 gene and breast cancer (p = 0.02). But, there were<br />no significant difference in IL-10 1082G→A genotypes frequency in breast cancer<br />when compared with normal individuals (p = 0.99). It's concluded that the CC<br />genotype and C allele of the G−174 C in the IL-6 gene were associated with breast<br />cancer in Egyptian women.
Cytokines,IL6,IL10,Gene polymorphism,breast cancer
https://besps.journals.ekb.eg/article_35550.html
https://besps.journals.ekb.eg/article_35550_1c6e5667da1cca561e3348af3c362947.pdf
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
32
1
2012
06
01
Some Biochemical Changes in Brain and Blood of Experimental Rats Resulting From Monosodium Glutamate (MSG) Administration
141
156
EN
Ayman
Wagih
Medical Biochemistry Department, Faculty of Medicine –Tanta University
10.21608/besps.2012.35555
Objective: Monosodium glutamate (MSG) is one of the commonest food additives in<br />the world and it is a commonly used flavor enhancer. In recent years MSG<br />consumption has increased as flavoring in cooking. Interestingly, little is known<br />about its effects on oxidative stress and some metabolic changes. Aim of the study:<br />The aim of this study was to elucidate the effect of oral ingestion of MSG on some<br />biochemical changes in the brain and blood of experimental rats to demonstrate the<br />adverse effects of that widely used flavor enhancer. Materials and methods: This<br />study was carried out on 50 white albino rats which were divided into two equal<br />groups; control group and MSG treated group. All groups were subjected to<br />estimation of serum lipid profile, free fatty acids (FFs) and malondialdehyde (MDA)<br />levels. Also brain tissue levels of L-glutamate, gamma amino butyric acid (GABA)<br />and MDA were measured for all groups. Results: The present study showed that oral<br />feeding of MSG significantly increased final body weight, visceral fat weight,<br />impaired glucose tolerance and a state of dyslipidemia with an increased serum free<br />fatty acid level. Significant increase of both serum and brain MDA tissue levels were<br />also documented. MSG-treated group also showed significant increase of brain L.<br />glutamate and significant decrease of brain gamma amino butyric acid (GABA) levels<br />as compared to the control. Conclusion: On the basis of these results it could be<br />concluded that MSG may be an inductive factor of several biochemical and metabolic<br />alterations taking place in healthy subjects, which clearly must contribute to the<br />development of obesity, decreased glucose tolerance and some excitotoxic effects.<br />Recommendations: The present findings are alarming, and throw doubts upon the<br />unscrupulousness of current use of the flavoring agent MSG in fast processed and<br />canned foods. Much work is still to be performed to determine other adverse effects<br />on other organs.
Monosodium glutamate (MSG),malondialdehyde (MDA) and gamma amino butyric acid (GABA) and free fatty acids (FFs)
https://besps.journals.ekb.eg/article_35555.html
https://besps.journals.ekb.eg/article_35555_5a184dac28b740dfd44723af34d4c50d.pdf
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
32
1
2012
06
01
Evaluation of Mammaglobin Transcription and its protein in Blood as a Marker For Diagnosis of Breast Cancer
157
172
EN
Hosam
El-Ezawy
Department of Clinical Biochemistry, National Liver Institute, Menoufiya University
Rayyh
Saleh
Clinical Pathology Department, Faculty of Medicine, Al-Azhar University
Mona
Abd El-Raof
Department of Public Health, National Liver Institute, Menoufiya University
Enas
Abd El-Razik
Department of Oncology , Faculty of Medicine, Menoufiya University
Mohamed
El Tahmody
Department of Pathology, Faculty of Medicine, Menoufiya University
Moones
Obada
Departments of Clinical
pathology, National Liver Institute, Menoufiya University
Hazem
El-Kashef
Radiology Department, Faculty of
Medicine, Cairo University
10.21608/besps.2012.35572
Breast cancer is the most common cancer and the second most common cause of<br />cancer death among women in the Western world. As understanding of the<br />pathophysiology of cancer increases, the role of tumor markers becomes more<br />important in the management of cancer patients. An ideal tumor marker should be<br />highly sensitive, specific, and reliable with high prognostic value, organ specific and<br />it should correlate with tumor stages. Human mammaglobin (hMAM) gene was<br />cloned in 1996, hMAM encodes a glycoprotein. The expression of hMAM was initially<br />believed to be restricted to the adult mammary gland and breast cancer cell lines. The<br />aim of the present study was to investigate the clinical reliability of each hMAM<br />mRNA and its transcripts as a tumor marker in breast cancer patients to diagnose<br />breast cancer. The subjects were selected from Oncology Department, Faculty of<br />Medicine Menoufiya University before hormonal, chemotherapy or surgical<br />treatment. Eighty six patients suffering from breast cancer and 59 subjects as a<br />control group were enrolled in this study. The control group was subdivided into 11<br />apparent healthy subjects (18.6%) and 48 patients served as patient control group<br />(81.4%) suffering from cancers rather than breast cancer. Results: A highly<br />significant increase in the mean serum level of hMAM was detected in the breast<br />cancer patients compared to the control group. Regarding expression of hMAM<br />mRNA, 50 females having breast cancer (73.5%) were positive, while it was negative<br />in 100% of females in the control group. This gave 73.5% sensitivity, 100%<br />specificity, 100% positive predictive value and 76% negative predictive value.<br />Conclusion: The expression of hMAM is restricted to breast epithelial cells. So,<br />hMAM is a promising marker of interest in breast cancer. Further studies are needed<br />to evaluate its usage for screening and staging of breast cancer.
https://besps.journals.ekb.eg/article_35572.html
https://besps.journals.ekb.eg/article_35572_7a35a473bdbfc8ce5aad5c9d2b2708ec.pdf
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
32
1
2012
06
01
In Vitro Study of the Effect of Cypermethrin on Human Sperm Function, DNA Fragmentation and the Possible Protective Role of Vitamins C and/ or E
173
189
EN
Adel
Zalata
Medical Biochemistry, Faculty of Medicine, Mansoura University
Hussein
Abdalla
Medical Biochemistry, Faculty of Medicine, Mansoura University
Mohamed
Serria
Medical Biochemistry, Faculty of Medicine, Mansoura University
Amal
Aziz
Clinical Pathology Dept. Faculty of Medicine, Cairo University
Sahar
El-Dakroory
Forensic Medicine & Clinical Toxicology, Faculty of Medicine, Mansoura
University, Egypt
Amal
El-Bakary
Forensic Medicine & Clinical Toxicology, Faculty of Medicine, Mansoura
University, Egypt
10.21608/besps.2012.35582
Pyrethroid pesticides are used preferably over organochlorines and organophosphates<br />due to their high effectiveness, low toxicity to non-target organisms and easy<br />biodegradability. Cypermethrin, a type II synthetic pyrethroid pesticide, is widely used<br />in Egypt in pest control programs in agriculture and in public health as well. The<br />objective of the present study was to evaluate the potential of cypermethrin<br />genotoxicity in sperm and to investigate the possible ameliorative effects of vitamin C<br />and/or E on cypermethrin toxicity. This study was done on semen samples collected<br />from 10 healthy normozoospermic volunteers. Each semen sample was divided into six<br />aliquots. One served as control negative aliquot (group I) that was not exposed to any<br />treatments. The second aliquot (group II) was incubated with 20 mM vitamin C<br />(ascorbic acid) and 2 mM vitamin E (α-tocopherol). The third aliquot was exposed to<br />cypermethrin with a dose of 10 μM (group III) for 6 hours at 37°C, while the other<br />three aliquots (IV, V, VI) were incubated with 20 mM vitamin C, 2 mM vitamin E and<br />vitamin C & E (20 mM, 2 mM) respectively for 30 min before cypermethrin exposure.<br />All aliquots were kept at room temperature. Unexposed and exposed aliquots were<br />analyzed for sperm concentration, motility, and viability according to WHO guidelines.<br />Hypo-osmotic Swelling (HOS) test and the modified alkaline comet assay were carried<br />out on the prepared samples. There was statistically significant decrease in parameters<br />of sperm motion, seminal functions and increase in sperm DNA damage parameters in<br />cypermethrin group. With addition of antioxidant vitamins C and E either alone or<br />combined there was statistically significant improvement in all of the parameters of<br />sperm motion, seminal functions and DNA damage parameters and the maximal<br />improvement was with the combined vitamin C and E. It could be concluded that<br />cypermethrin can alter sperm function and induce genotoxic effect on sperms in vitro<br />and that the antioxidant vitamins (C and E) might be useful in antagonizing the toxic<br />effects of cypermethrin on sperm.
https://besps.journals.ekb.eg/article_35582.html
https://besps.journals.ekb.eg/article_35582_b6de0188c0621c0e3a1162a1a996baa1.pdf
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
32
1
2012
06
01
Follicle-Stimulating Hormone Receptor Polymorphisms and Its Association with Ovarian Carcinoma
191
206
EN
Amal
Mackawy
Medical Biochemistry and Molecular Biology Department,
Faculty of Medicine, Zagazig University
Wafaa
Emam
Medical Biochemistry and Molecular Biology Department,
Faculty of Medicine, Zagazig University
10.21608/besps.2012.35586
Objectives: Follicle stimulating hormone (FSH) and its receptor (FSHR) are<br />important in ovarian follicular development and can influence the growth of ovarian<br />epithelial cells. It seems to implicate in ovarian carcinogenesis. Thr307Ala and<br />Asn680Ser are two single nucleotide polymorphisms (SNPs) of the FSHR gene which<br />have effects on FSH efficacy. Our aim was to examine the association between these<br />two SNPs of FSHR gene and the risk of epithelial ovarian carcinoma in Egyptian<br />females. Subjects and Methods: Genomic DNA was extracted from 40<br />histopathologically confirmed ovarian cancer patients and 20 cancer-free control<br />subjects using Polymerase chain reaction (PCR) assays with restriction<br />fragment length polymorphism (RFLP). Results: showed a non-significant association<br />between the genotypes with tumor stage for SNPs Ala307Thr and Ser680Asn<br />(P>0.05). The 307Ala and 680Ser carriers had higher risk to develop ovarian cancer<br />when compared with the controls (X2=3.935,,P =0.047, OR =2.81, 95% CI =0.99–<br />8.02; and X2=5.26, P=0.022, OR=3.491, 95% CI =1.158–10.526,respectively). The<br />genotypes of the two SNPs were significantly associated with the serous (SC) and<br />mucinous (MC) subtypes (X2=15.597, P=0.000 and X2=19.858, P = 0.000,<br />respectively), with non-significant associations in endometrioid (EC) and clear cell<br />(CC) subtypes (P>0.05). The two SNPs were found to be in modest linkage<br />disequilibrium, D 0.182 = ׳ and 0.1, r2 = 0.553 and 0.333 for the cancer and control<br />groups, respectively. Haplotype Ala307-Ser680 was shown to be associated with<br />higher risk of ovarian cancer (X2=5.79, P=0.026, OR=0.303, 95% CI =0.111–0.825),<br />with more association with SC and MC subtypes (X2=0.213, P= 0.002, OR = 0.184,<br />95% CI =0.062–0.543), in the EC and CC subtypes this haplotype showed no<br />significant correlation (P>0.05). Conclusion: SNPs at these two sites of FSHR may<br />influence FSHR function and enhance the probability to specific subtypes of ovarian<br />cancer. They may be useful as a DNA-based diagnostic biomarker for identifying<br />high-risk Egyptian females susceptible to ovarian cancer. SC and MC ovarian cancer<br />may have different carcinogenetic pathways when compared with EC and CC<br />carcinomas in Egyptian females.
FSHR,Single nucleotide polymorphism,ovarian carcinoma predisposition
https://besps.journals.ekb.eg/article_35586.html
https://besps.journals.ekb.eg/article_35586_1b017a5340fc32d4ce4f040cf855f87f.pdf
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
32
1
2012
06
01
HSP70 gene Polymorphism in Pre-eclamptic Women and its Correlation with Serum Levels of HSP70
207
218
EN
Ayman
Darwish
Department of Medical Biochemistry,
Faculty of Medicine, Alexandria University, Egypt
Burhan
Imamwerdi
Department of
Medical Lab. Technology, College of Applied Medical Sciences,
King Faisal University, KSA
Mahmoud
Melis
Department of Obstetrics & Gynaecology,
Faculty of Medicine, Alexandria University, Egypt
Fathia
AlJama
Department of Obstetrics &
Gynaecology , College of Medicine, King Faisal University, KSA
10.21608/besps.2012.35598
Objective: The present study was designed to evaluate the possible association of<br />three different HSP70; HSPA1A, HSPA1B and HSPA1L; gene polymorphisms with<br />pre-eclampsia and the possible correlation with serum levels of HSP70. Method: The<br />study included 46 women with pre-eclampsia as group I (GI) and twenty two<br />normotensive pregnant controls representing group II (GII). All candidates of the<br />study were subjected to the following: Measurement of human HSP70 concentrations<br />in serum, molecular analysis for PCR amplification of the HSPA1A G (190)C regions,<br />HSPA1B A(1267)G regions, HSPA1L T(2437)C regions. Results: Serum HSP70 was<br />found to be significantly increased in pre-eclamptic patients when compared to<br />normal pregnancy. HSPA1A (190) GC and HSPA1B (1267) GG genotypes occurred<br />more frequently in pre-eclamptic patients compared to healthy controls (p < 0.03).<br />Significant difference was found in the distribution of HSPA1B (1267) AG genotype<br />between the pre-eclamptic and control group (p < 0.004). Distribution of HSPA1L<br />T(2437)C gene was found similar in the Pre-eclamptic and control group. Cases with<br />HSPA1A G(190)C had a mean serum level of HSP70 that is 1.4 ng/ml more than<br />those without HSPA1A G(190)C polymorphism. A significant negative association<br />was noted between maternal age and serum Hsp70 concentration in both preeclampsia<br />and healthy pregnant women. Conclusion: Elevated serum HSP70 level in<br />pre-eclamptic patients seems to reflect the burden of oxidative stress taking place im<br />pre-eclampsia. HSP70 polymorphism may affect the serum level as proved by<br />increase in the mean serum level of HSP70 by 1.4 ng/ml in those with HSPA1A<br />G(190)C polymorphism .
Pre-eclampsia,Heat shock proteins,Heat Shock Protein 70,Polymorphisms
https://besps.journals.ekb.eg/article_35598.html
https://besps.journals.ekb.eg/article_35598_368240ea633c3d9e4f1fc0339abea4db.pdf