Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
30
1
2010
12
01
Renal Functions in Pediatric Patients with β-Thalassemia Major: Relation to Iron Chelation Therapy
1
20
EN
Enas
Hamed
Department of Physiology , Faculty of
Medicine, Assiut University.
eah3a2010@yahoo.com
Nagla
ElMelegy
Department of Medical Biochemistry, Faculty of Medicine, Assiut University.
10.21608/besps.2010.36162
Renal failure is one of the main complications in β-thalassaemia as a result of longstanding<br />anemia, therefore the objectives of the study were: (1) To investigate<br />glomerular and tubular functions in transfusion dependant (TD) β-thalassaemia<br />major (βTM) pediatric patients without any occult renal diseases. 2) To correlate the<br />findings with clinical parameters, oxidative stress status [by measuring serum total<br />antioxidant capacity (TAC) and urinary malondialdehyde (MDA)] and<br />desferrioxamine (DFO) chelation therapy. Patients and methods: The study included<br />sixty-nine TD-βTM patients (45 males and 24 females). They were subdivided into<br />those with (34 patients) and without chelation therapy (35 patients). In addition to<br />fifteen age-, sex-, body mass index (BMI)-matched healthy subjects as a control<br />group. From each participant blood sample was taken for determination of the serum<br />(S) levels of creatinine (Cr), albumin, calcium (Ca), inorganic phosphorus (PO4), uric<br />acid (UA), cystatin-C (CysC) and TAC. Also a urine sample was taken for<br />determination of urinary (U) levels of creatinine, albumin, N-acetyl-beta-Dglucosaminidase<br />(NAG) activity, β2-microglobulin (β2MG) and MDA. Results: The<br />results revealed that in βTM patients the serum levels of Cr, albumin, PO4, UA, CysC,<br />and urinary levels of NAG/Cr, β2MG/Cr, MDA/Cr, albumin/Cr were significantly<br />higher; while serum TAC, estimated glomerular filtration rate (eGFR) were<br />significantly lower than those of the controls. In patients with chelation therapy,<br />serum levels of CysC, and albumin were significantly higher while, TAC was<br />significantly lower than those without chelation therapy. Significant positive<br />correlations were observed in TD-βTM patients between SCysC and each of Salbumin<br />and SCr; STAC and eGFR and UNAG/Cr with each of Uβ2MG/Cr, UMDA/Cr and<br />Ualbumin/Cr. Also, significant negative correlations were found between SCysC and<br />eGFR; STAC and each of SCys, SCr, and Salbumin. Conclusion: The results of the<br />present study confirm that renal tubular dysfunction exists in children with βTM<br />which could be attributed to iron overload, oxidative stress and DFO therapy.
β-thalassaemia major,β2-microglobulin,Cystatin-C,Desferioxamine,Nacetyl- beta-D-glucosaminidase activity,Oxidative Stress
https://besps.journals.ekb.eg/article_36162.html
https://besps.journals.ekb.eg/article_36162_a32dba85a3f65c4c4019ecf71261a5f0.pdf
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
30
1
2010
12
01
Assessment of the Role of Maternal Angiogenic Factors and Nitric Oxide in Prediction of Preeclampsia
21
36
EN
Olfat
Shaker
Department of Medical Biochemistry , Faculty of Medicine, Cairo University
Fatma
Taha
Department of Medical Biochemistry , Faculty of Medicine, Cairo University
Salwa
Fayez
Department of Medical Biochemistry , Faculty of Medicine, Cairo University
Mohmmed
Shehata
Department of Obstetrics & Gynecology , Faculty of Medicine, Cairo University
Dalia
Ahmed
Department of Public Health & Community Medicine , Faculty of Medicine, Cairo University
10.21608/besps.2010.36163
Preeclampsia has been proposed to be an antiangiogenic state that may be detected by the determination of the concentrations of the soluble vascular endothelial growth factor receptor- 1 (sVEGFR-1) and placental growth factor (PlGF) in maternal blood even before the clinical development of the disease. Aim: The aim of the present study was to determine the role of the combined use of uterine artery Doppler velocimetry (UADV) and maternal plasma PlGF , sVEGFR-1 and NO products concentrations for the prediction of preeclampsia in high-risk women.and to compare these parameters between patients with mild and severe preeclampsia. Subjects and Methods: A prospective cohort study was conducted on 142 women, only 112 were enrolled in the study, patients with preeclampsia were subclassified as either severe or mild preeclampsia. Blood samples were obtained between 22 and 26 weeks of gestation. Doppler ultrasound of the uterine arteries at the time of blood sampling was done. The presence of an early diastolic notch in the uterine arteries was determined. An abnormal UADV was defined as the presence of bilateral uterine artery notches and/or a mean pulsatility index above 95th percentile for the gestational age. Maternal serum PlGF and sVEGFR-1 concentrations were determined with the use of sensitive and specific immunoassays. Nitric Oxide Colorimetric Assay was used also to measure NO products in the maternal blood. Results: Among patients with abnormal UADV, maternal plasma sVEGFR1, PlGF and NO products concentrations contributed significantly in the identification of patients destined to develop mild preeclampsia and severe preeclampsia sVEGFR1 (>2005 pg/ml) and NO products (<50.90 umol/L) were found to be the best predictors for preeclampsia with high sensitivity and specificity followed by PLGF (<286.32 pg/ml). In severe preeclampsia sVEGFR1 (>2900 pg/ml) was the best followed by NO products (<54 umol/L) and PLGF (<234.56 pg/ml). Conclusion: The results of current study suggested that the identification of high concentrations of sVEGFR1 combined with low concentrations of PlGF and NO products, could be used to predict the development of preeclampsia.
preeclampsia,soluble vascular endothelial growth factor receptor- 1 (sVEGFR1),placental growth factor (PlGF),Nitric oxide (NO)
https://besps.journals.ekb.eg/article_36163.html
https://besps.journals.ekb.eg/article_36163_8ad7028228127eb836117684c4a41422.pdf
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
30
1
2010
12
01
Gly82Ser Polymorphism of the Receptor for Advanced Glycation End Products Gene (RAGE) and the Endogenous Secretory RAGE (esRAGE) and its Association with Diabetic Retinopathy in Type 2 Diabetic Patients
37
52
EN
Yousri
Hussein
Faculty of Medicine. Zagazig University. Medical Biochemistry
Department
Etewa
L
Faculty of Medicine. Zagazig University. Medical Biochemistry
Department
Bahaa El Din
Hassan
Faculty of Medicine. Zagazig University. Opthalmic Department
10.21608/besps.2010.36164
The binding of advanced glycation end-products (AGEs) to their receptor (RAGE)<br />may play an important role in the development of diabetic retinopathy (DR). Recently,<br />endogenous secretory RAGE (esRAGE) has been identified as an alternative splicing<br />form of RAGE able to capture AGEs, and exerts protection against AGEs-induced<br />endothelial cell injury. A Gly82Ser polymorphism in exon 3 of RAGE gene was<br />identified and thought to have an effect on the functions of its protein. This study was<br />planned to investigate the frequency of the Gly82Ser polymorphism in RAGE gene<br />and the role of esRAGE as a biological marker for DR in type2 diabetes and its<br />association with the severity of DR. Thirty-five patients with type2 diabetes were<br />recruited into the study. They were subclassified into 15 patients with no clinically<br />apparent retinopathy (No DR), 12 patients with nonproliferative DR (NPDR), and 8<br />patients with proliferative DR (PDR). Twenty, age matched, healthy subjects were<br />included as controls. Serum esRAGE level was measured by enzyme-linked<br />immunosorbent assay. Genotype frequencies of Gly82Ser polymorphism were studied<br />by polymerase chain reaction amplification and restriction fragment length<br />polymorphism analysis using AluI enzyme. The results showed no significant<br />difference between serum esRAGE levels in both controls and diabetic patients with<br />No DR (P = 0.15). Among the diabetic subjects, there was a significant decrease of<br />serum esRAGE levels between patients with No DR and patients with NPDR (P =<br />0.008) and a more significant decrease between diabetic patients with No DR and<br />patients with PDR (P = 0.001). The low serum esRAGE diabetic patients had higher<br />risk to develop DR than those with high serum esRAGE level (odds ratio = 4.7, 95%<br />confidence interval = 1.07-20.65, P = 0.02). There were no significant differences in<br />genotyping frequencies or allele frequencies between controls and diabetic patients<br />with No DR, or patient with DR (P<0.05). In conclusion, serum esRAGE level showed<br />a significant association with the severity of DR and, hence, it could be used as a<br />prognostic tool to predict the development and progression of DR. esRAGE could be<br />a novel and potential protective factor for DR. Gly82Ser polymorphisms in RAGE<br />gene are not associated with the susceptibility of type 2 diabetes, or with the<br />development of DR in type 2 diabetic subjects.
https://besps.journals.ekb.eg/article_36164.html
https://besps.journals.ekb.eg/article_36164_49d9c0ad97f4593594703260291f2bca.pdf
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
30
1
2010
12
01
Role of Interleukin-18, S-adenosylmethionine and SAdenosylhomocysteine as Cardiovascular Risk Factors in Patients with Systemic Lupus Erythematosus
53
72
EN
Ayman
El Samanoudy
Department of Medical Biochemistry, Faculty of Medicine, Mansoura University
Adel
Zalata
Department of Medical Biochemistry, Faculty of Medicine, Mansoura University
Abd El Hameed
Metwali
Department of Internal medicine, Faculty of Medicine, Mansoura University
Fayez
el Kenawy
Department of Internal medicine, Faculty of Medicine, Mansoura University
Adel
Elbadrawy
Department of Diagnostic Radiology, Faculty of Medicine, Mansoura University
10.21608/besps.2010.36165
Background: The incidence of Systemic Lupus Erythematosus (SLE) appears to be<br />increasing and the main cause of death in that disease is coronary artery disease<br />since SLE is associated with premature atherosclerosis. The association of plasma<br />interleukin-18 levels and proinflammatory cytokines with cardiovascular risk in SLE<br />patients has not been extensively established. Hyperhomocysteinemia is associated<br />with increased risk for cardiovascular events, but it is not clear whether it is a marker<br />or mediator for vascular dysfunction or a marker for another risk factor. Aim of the<br />work: The purpose of the present study was to determine whether plasma IL-18, SAM,<br />SAH and SAM/SAH ratio are associated with cardiovascular risk factors and disease<br />activity in SLE patients. Subjects and Methods: The plasma concentrations of a novel<br />pro-inflammatory cytokine, interleukin (IL)-18 by ELISA as well as SAM,SAH and<br />SAM/SAH ratio by HPLC was determined in 31 patients with systemic lupus<br />erythematosus (SLE) and 30 sex- and age-matched healthy control subjects and<br />correlated them with cardiovascular risk factors and the SLE disease activity. For<br />every patient the systemic lupus disease activity was assessed using the Systemic<br />Lupus Erythematosus Disease Activity Index (SLEDAI). Body mass index (BMI),<br />systolic blood pressure, diastolic blood pressure, CBC, liver functions, plasma<br />creatinine, urine analysis, erythrocyte sedimentation rate (ESR)1, ANA, anti- ds DNA,<br />C3, C4, fasting insulin and glucose, plasma lipid profile, plasma SAH,SAM<br />,SAM/SAH ratio, titers of autoantibodies against oxidized low-density lipoprotein and<br />carotid intima media thickness (CIMT) were determined. SLE patients with a history<br />of diabetes mellitus, hypertension, hyperlipidemia, smoking, or coronary artery<br />disease (CAD) and positive pregnancy test were excluded. Results: The mean age of<br />SLE patients was 35.1±10.3 years and the mean duration of SLE was 4.2± 2.9 years.<br />Plasma concentrations of IL-18 were significantly higher in SLE patients than agematched<br />healthy controls (p< 0.001). Also, plasma SAH is elevated in SLE patients<br />versus controls while SAM and SAM/SAH ratio were significantly lower in SLE<br />patients versus controls. Elevation of plasma IL-18 correlated positively and<br />significantly with SLE disease activity index. In addition, plasma concentrations of<br />IL-18 correlated positively and significantly with BMI, insulin, Homeostasis model<br />assessment insulin resistance (HOMA IR), triglycerides, CIMT, and SAH, in SLE<br />patients. IL18 concentrations showed a positive and significant correlation with
plasma creatinine (r=0·7, P = 0·001), antinuclear antibody (ANA) (r= 0.6, p=0.001),<br />anti double stranded DNA (dsDNA) (r=0.5, p=0.008), ESR1 (r= 0.56, p=0.001). The<br />concentrations of plasma IL-18 in SLE patients with elevated plasma creatinine were<br />significantly higher than those with normal plasma creatinine (285.7.6±59.6 pg/ml vs<br />182.8±29.4, p< 0.001). Also, SLEDAI correlated positively with both plasma levels of<br />insulin and HOMA-IR values (p<0.05 in both). Conclusions: In SLE patients, a high<br />IL-18 level reflects activity of the disease and is related to cardiovascular risk factors.<br />IL-18 is therefore suggested to play a crucial role in triggering the inflammatory<br />processes of premature atherosclerosis in SLE, in addition to the markers of<br />disturbed homocysteine metabolism could play a role as mediator of cardiovascular<br />disorders in SLE.
IL 18,SAH/SAM ratio,Systemic lupus erythematosus,disease activity,Atherosclerosis,T-helper cell cytokines
https://besps.journals.ekb.eg/article_36165.html
https://besps.journals.ekb.eg/article_36165_fe5ccdd8ff640a667b14004e282cf5c2.pdf
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
30
1
2010
12
01
The Role of Urinary Fibronectin and Telomerase in Patients with Superficial Bladder Cancer after Transurethral Resection Treated with BCG
73
102
EN
Ghada
Abdel Aleem
Medical Biochemistry Department, Faculty of Medicine, Tanta University
Hala
Hamouda
Medical Biochemistry Department, Faculty of Medicine, Tanta University
Tarek
Gameel
Urology Department, Faculty of Medicine, Tanta University
Mona
Abdel-Azem
Pathology Department, Faculty of Medicine, Tanta University
10.21608/besps.2010.36166
Objective: Bacillus Calmette-Guérin (BCG) has been shown to be an effective treatment for superficial transitional cell carcinoma (TCC) of the bladder, but the precise mechanism of action of BCG remains poorly understood. Fibronectin (FN) has been found to play a role in BCG therapy. Although adjuvant BCG has been shown to improve the clinical outcome of superficial bladder cancer (SBC), a significant proportion of patients fail to respond to it. Identification of the subset of patients that are going to develop recurrent disease or stage progression after BCG therapy is very important. Telomerase activity represents a potential tool for tumor detection. The aim of the present study was to give an insight into the diagnostic and prognostic values of the quantitative urinary estimation of both fibronectin (FN) and the catalytic subunit of the complex human telomerase reverse transcriptase [hTERT]) as non invasive tumor markers for early detection of patients with high risk superficial transitional cell carcinoma (TCC) and after trans-urethral resection of bladder tumors (TURBT) that received adjuvant BCG immunotherapy to explore its association with recurrence-free-survival (RFS) or development of invasive disease. Materials and methods: The study was carried out on 10 healthy individuals as a control group (group I) and 20 Egyptian patients with histologically confirmed superficial bladder cancer (group II). Patients were underwent formal TURBT. Morning urine samples were collected from every patient, 1 day before and 2days, two weeks, six weeks, three months, six months and one year after TURBT. Also, morning urine samples were collected from the healthy individuals. Urine samples were used for cytological examination, estimation of fibronectin level using ELISA technique and for the quantitative analysis of hTERT using quantitative real time RT-PCR technique. Results: The results showed that urinary fibronectin level and normalized hTERT- mRNA were significantly higher in SBC patients before TURBT (group II) versus control group (P<0.001). hTERT-mRNA and urinary fibronectin levels were significantly different in group II before and 2 days after TURBT. Urinary fibronectin and normalized hTERT- mRNA were significantly lower 2 weeks and 6 weeks after TURBT than patients group 2 days after TURBT. Both markers were significantly lower in fifteen patients 3 months, 6 months and one year after TURBT when compared with their levels 2 days after TURBT. There was insignificant difference of both markers between the control group and fifteen patient one year after TURBT. The remaining five patients suffered recurrence of tumor. This approach enabled us to identify cutoff values of urinary fibronectin which characterized by 90% sensitivity and 100% specificity and 93.3% accuracy, and that for urinary normalized hTERT with 85% sensitivity and 80% specificity and 83.3% accuracy. Conclusion: On the basis of these results, it could be concluded that both urinary fibronectin and hTERT- mRNA can be considered from the best markers for diagnosing superficial bladder carcinoma. These parameters can be also used to determine the presence of residual tumor load after TURBT; also they can give an insight about the expected response to BCG immunotherapy after TURBT and detect the BCG nonresponder patients. It seems conceivable that these emerging urine-based tests may progressively replace urine cytology and form a reliable adjunct to cystoscopy in the diagnosis and follow up of patients with superficial bladder cancer.
Bacillus Calmette-Guérin (BCG),Superficial bladder cancer (SBC),Fibronectin (FN),transitional cell carcinoma (TCC),carcinoma in situ (CIS),human telomerase reverse transcriptase (hTERT),transurethral resection of bladder cancer (TURBT)
https://besps.journals.ekb.eg/article_36166.html
https://besps.journals.ekb.eg/article_36166_2c3d16c32476e334f4fd7db8040e1d3b.pdf
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
30
1
2010
12
01
Some Potential Biochemical Markers of Leprosy
103
122
EN
Ghada
Abdel Aleem
Medical Biochemistry Department, Faculty of
Medicine, Tanta University
Hoda
Moneib
Dermatology & Venereology Dep;
Faculty of Medicine, Ain Shams University
Sahar
Youssef
Dermatology & Venereology Dep;
Faculty of Medicine, Ain Shams University
Nahla
Darwish
Dermatology & Venereology Dep;
Faculty of Medicine, Ain Shams University
Mona Abdel-Azem
Mona Abdel-Azem
Pathology Dep., Faculty of Medicine, Tanta University
10.21608/besps.2010.36167
Background: Leprosy is a chronic granulomatous infectious disease caused by the<br />bacterium Mycobacterium leprae. Leprosy "Type 1" reactions (T1R), reversal<br />reactions, occur in 30–40% of borderline patients with cellular immune responses to<br />M. leprae. "Type 2" reactions (T2R), also known as erythema nodosum leprosum<br />(ENL), occur only in lepromatous (LL) and borderline lepromatous (BL) patients with<br />a high bacterial load and little or no cellular immunity to M. leprae. Corticosteroids<br />alleviate symptoms in T1R and T2R, but many patients have multiple, recurrent<br />episodes. The Objective of the present study is to verify the validity of measuring<br />chitotriosidase activity and neopterin level, products of activated macrophages,<br />adenosine deaminase activity and monocyte chemoattractant protein-1 (MCP-1) as<br />markers of leprosy and to detect their values in diagnosis of different types of leprosy.<br />Methods: This study was conducted on 15 healthy subjects and 75 leprotic patients<br />that were classified into 5 groups [tuberculoid leprosy (TT), borderline tuberculoid<br />(BT), borderline borderline (BB), borderline lepromatous (BL), and lepromatous<br />leprosy (LL)], each group formed of 15 patients, depending on clinical,<br />bacteriological and histopathological pattern. Patients were further grouped with a<br />BI≥2 as multibacillary (MB, n=45), whereas those with BI<2 were grouped as<br />paucibacillary (PB, n=30). Thirty-four of the aforementioned patients were<br />diagnosed with reactions of which 17 had type II/erythema nodosum leprosum (ENL)<br />and 17 had type I/reversal reaction (RR). Reactions were treated using prednisolone<br />for 12 weeks. Venous blood sample was collected from each subject and processed for<br />estimation of the activity of chitotriosidase and adenosine deaminase, neopterin and<br />MCP-1 levels. Results: both chitotriosidase activity and neopterin level were elevated<br />in leprosy patients with significant elevation in MB than PB leprosy with significant<br />lowering in the patients with reactional leprosy after prednisolone therapy.<br />Adenosine deaminase activity was significantly elevated in TT, PB and reactional<br />leprosy. MCP-1 was significantly elevated in LL, MB and ENL. Conclusion:<br />chitotriosidase and neopterin could be considered as promising markers for<br />differentiation of MB from PB patients and useful for determining the response of<br />reactional leprosy to therapy. Adenosine deaminase could be useful in distinguishing<br />TT, PB and reactional leprosy. MCP-1 could be considered a fair marker in LL, MB<br />and ENL. In addition, these findings may provide new clues to the pathogenesis of<br />leprosy reactions.
multibacillary (MB),paucibacillary (PB),Non-reactional leprosy (NR),erythema nodosum leprosum (ENL),reactional leprosy (RL),reversal reaction (RR) and healthy controls (HC). Tubercloid Leprosy (TT),Leprometous Leprosy (LL),Bordreline Lepromatous (BL),Bordreline Tubercloid (BT),Bordreline Bordreline (BB)
https://besps.journals.ekb.eg/article_36167.html
https://besps.journals.ekb.eg/article_36167_90fd0e683b9debbb986b34d871e59b8a.pdf
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
30
1
2010
12
01
Effect of Histidine on Autotaxin Activity in Experimentally Induced Liver Fibrosis
123
136
EN
Manal
El-Batch
Medical Biochemistry Department, Faculty of Medicine, Tanta University
Wafaa
Ibrahim
Medical Biochemistry Department, Faculty of Medicine, Tanta University
Soha
Said
Medical Biochemistry Department, Faculty of Medicine, Tanta University
10.21608/besps.2010.36168
The aim of the present study was to explain whether serum autotaxin activity might be<br />a target for regulation of liver fibrosis and to evaluate the hepato-protective and antifibrotic<br />effects of histidine in thioacetamide induced liver fibrosis in rats. The study<br />was carried out on 100 albino rats, classified into 5 groups each of 20 rats: group І<br />(control group), group ІІ: rats were given histidine intra-peritoneally, group ІІІ: rats<br />were injected intra-peritoneally with thioacetamide, group IV: rats were injected with<br />L-histidine together with thioacetamide, group V: rats were injected with TAA for one<br />month then treated with intra-peritoneal injection of L-histidine for another month. At<br />the end of experiment, blood and liver were collected for determination of some liver<br />enzymes, plasma total antioxidant capacity, serum autotaxin activity and liver tissue<br />hydroxyproline. Thioacetamide treatment caused significant increases in liver<br />enzymes, autotaxin activities and liver hydroxyproline, but with a significant decrease<br />in plasma total antioxidant capacity. Upon treatment with histidine significant<br />decreases in liver enzymes, autotaxin activities and liver hydroxyproline were<br />observed with a significant increase in plasma total antioxidant capacity in group IV<br />and significant decrease in group V. Conclusion: histidine as an antioxidant has a<br />protective effect on thioacetamide-induced liver fibrosis , its beneficial effects in rats<br />might be not only by inhibition of collagen synthesis and increasing total antioxidant<br />capacity, but also by inhibition of autotaxin activities, thus reducing its capacity to<br />produce lysophosphatidic acid which has a role in liver fibrosis.
autotaxin,histidine,Liver fibrosis,Thioacetamide,total anti-oxidant capacity
https://besps.journals.ekb.eg/article_36168.html
https://besps.journals.ekb.eg/article_36168_d6c6825fcd2917018696af46cecebf21.pdf
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
30
1
2010
12
01
Biochemical Studies on the Molecular Level of Polioviruses
137
148
EN
Hend
El Sherbini
Clinical Pathology Department, Faculty of Medicine, Cairo University
Naglaa
Abd El Meguid
The Holding Company for Biological Products and Vaccines (VACSERA)
10.21608/besps.2010.36169
Paralytic poliomyelitis continues to be an important disease affecting many children.<br />The strategy for poliomyelitis eradication includes routine immunization, surveillance<br />of acute flaccid paralysis (AFP) and mopping-up activities. Environmental<br />monitoring, mainly involving the screening of waste water can provide evidence of<br />the circulation or absence of wild-type polioviruses. The present work has been<br />carried out to investigate the sequence divergence of neurovirulent derivatives of the<br />Sabin oral poliovirus vaccine (OPV) strains, to establish epidemiological link<br />between isolates, and to determine poliovirus genotype which circulates in Egypt.<br />Enzyme Linked Immunoassay (ELIZA) and Reverse Transcription Polymerase Chain<br />Reaction (RT-PCR) for intratypic differentiation have been used. Molecular analysis<br />of the VPI/2A junction region has been performed on 22 isolates using the dideoxy<br />chain termination method. Sequencing of the amplified region has been performed<br />using the automated genetic analyzer ABI prism 310. Forward and reverse<br />electropherograms were compared using sequencing alignment software version 2.<br />Phylogenetic analysis of the VPI/2A region was done using Clustal X software.
Sequence divergence,neurovirulent,epidemiological link,genotype,VPl/2A junction region,Phylogenetic analysis
https://besps.journals.ekb.eg/article_36169.html
https://besps.journals.ekb.eg/article_36169_3790caccac133746e305dc5c515d6e27.pdf
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
30
1
2010
12
01
Identification of A 38 KDa Antigen in the Urine of Schistosoma Mansoni Chronicaily Infected Patients Using A Specific Antibody Microeluted from A Nitrocellulose Membrane; A Diagnostic Tool
149
158
EN
Samir
Mahgoub
Department of Biochemistry, Faculty of Medicine, Al-Minia University
10.21608/besps.2010.36170
Schistosomiasis is a chronic debilitating disease affecting 200 -300 million<br />throughout the world, a major focus of research has been done to identify and<br />characterize antigens that may have vaccine and/or diagnostic potential. In the<br />present study, NP-40 extracted surface proteins of Schistosoma mansoni (S.<br />mansoni) adult worms were subjected to 12.5% Sodium Dodecyl Sulfate–<br />Polyacrylamide Gel Electrophoresis (SDS-PAGE) and electrotransfered onto a<br />nitrocellulose membrane (PVDF), then, incubated with pooled sera collected from S.<br />mansoni chronically infected patients. The presence of antibodies identify a number<br />of NP-40 extracted surface proteins of the adult worms of S. mansoni. One of these<br />proteins of 38 kDa molecular weight with high immunogenicity was selected. The<br />strip of nitrocellulose membrane containing the complex of the identified protein and<br />its specific antibody was cut guided by the molecular weight marker, then, the<br />antibody was micro-eluted. Proteins of urine samples from the same patients were<br />precipitated and purified over G-Sephadex column. The purified proteins of urine<br />samples and proteins of sera were subjected to another SDS- PAGE and electrotransferred<br />onto PVDF membrane. The microeluted antibody was used to identify an<br />antigen of 38 kDa molecular weight in sera of the chronically infected patients as<br />well as in urine. The 38 kDa antigen was excreted in the urine of those patients in a<br />stable form and detected by the specific monoclonal antibody. The active epitope of<br />38 kDa antigen could be a promising immunochemical probe for S. mansoni<br />infection diagnosis. Further studies will be done to characterize that antigen as well<br />as its potential application in immunodiagnosis for S. mansoni
https://besps.journals.ekb.eg/article_36170.html
https://besps.journals.ekb.eg/article_36170_d388d53329cb1ee5ce6d1917910c98a6.pdf
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
30
1
2010
12
01
Molecular Characterization of the Gene Encoding SJCHGC 03921 Protein of the Lung Stage of Schistosoma Mansoni (7-Days Schistosomula)
159
170
EN
Samir
Mahgoub
Department of Biochemistry, Faculty of Medicine, Al-Minia University
10.21608/besps.2010.36171
The parasitic helminth Schistosoma mansoni (S. mansoni) is a major public health<br />concern in many developing countries. Over 200 million people have, and another<br />600 million are at risk of contracting schistosomiasis which is one of the major<br />neglected tropical diseases. For this dangerous disease the development of long -<br />lasting immunity through vaccination may be the real solution to control the spread<br />of the disease. The molecules on the surface or associated with the tegument of S.<br />mansoni are a major focus as potential vaccine candidates. In the present study, all<br />surface and internal proteins of the lung stage of the parasite were screened to<br />increase the chances for the discovery of a unique protein of the parasite to be<br />targeted by the immune system of the host. Pooled sera were collected from S.<br />mansoni chronically infected patients, then, purified over a column made of soluble<br />extract of the lung stage (7-days schistosomula) of S. mansoni. The eluted antibodies<br />were used to immunoscreen λgt11 cDNA library of 7-days schistosomula. A number<br />of cDNA clones were identified after three rounds of immunoscreening and plaques<br />purification. The phage DNAs of the isolated clones were amplified by polymerase<br />chain reaction (PCR) using λgt11 forward and reverse primers, then, cloned in<br />PCRTMII plasmid vector. The isolated clone 4-65 was fully sequenced and was found<br />encoding the gene of SJCHGC 03921 protein of 7-days schistosomula of S. mansoni.<br />Also, the 0.9 kb cDNA clone was found to have a single open reading frame (ORF)<br />encoding 269 amino acids, which exhibited 94% homology with the gene of SJCHGC<br />03921 protein of Schistosoma japonicum.
https://besps.journals.ekb.eg/article_36171.html
https://besps.journals.ekb.eg/article_36171_7853ebaf3768780050d08a891edc6adb.pdf
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
30
1
2010
12
01
Schistosoma Mansoni: Partial Molecular Characterization of the Gene Encoding Zinc Finger Protein of the Lung Stage (7-Days Schistosomula)
171
184
EN
Samir
Mahgoub
Department of Biochemistry, Faculty of Medicine, Al-Minia University
10.21608/besps.2010.36172
Schistosomiasis is a serious parasitic disease with world-wide distribution, causing<br />an estimated 200 000 deaths per year. Despite the fact that the global distribution of<br />schistosomiasis has changed significantly in the past 50 years, particularly in regions<br />where control strategies have been successfully employed, the disease remains<br />endemic in over 70 developing countries and more than 200 million people are<br />estimated to be harboring the disease. Chemotherapy, although effective, it does not<br />prevent re-infection, and in addition, partial drug resistance may occur. Hence,<br />immunological intervention in the form of a vaccine would contribute to the success<br />of the present efforts. Most of the trials in the development of anti-schistosomiasis<br />vaccine were involving membrane-associated antigens contained in the adult<br />Schistosoma mansoni (S. mansoni) tegument because they are capable of<br />stimulating protective immunity. In the current study, a trial to obtain antigen<br />varieties which could be vaccine candidates by incorporating internal antigens of the<br />lung stage of S. mansoni (7-days schistosomula) in addition to the tegumental<br />antigens was done. The soluble extract of the lung stage was obtained by sonicating<br />the whole parasite, then, coupled to Sepharose-4B column for affinity purification of<br />pooled sera collected from chronically infected patients. The purified sera were used<br />to immunoscreen λgt 11 cDNA library of 7-days schistosomula. The plaques<br />purification after three rounds of immunoscreening gave a number of cDNA clones.<br />one of the isolated clones (clone 2-4) was amplified by PCR using λgt 11<br />forward and reverse primers, then ,cloned in a plasmid vector (PCRTMII). The<br />cloned insert was partially sequenced 270 bp from the 5/- end using Sp6 primer as<br />well as 187 bp from the 3/-end using T7 primer. The sequenced part of the clone<br />showed it has two open reading frames (ORFs) with 31-36% homology to the gene<br />that encodes Zinc Finger protein (the transcriptional regulatory protein) from a<br />number of eukaryotic species including human , rat and mice.
https://besps.journals.ekb.eg/article_36172.html
https://besps.journals.ekb.eg/article_36172_2cb77f452c0779c6b3dbc53d4a6fcb50.pdf
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
30
1
2010
12
01
Renal Actions of Neutral Endopeptidase Inhibition and Its Effects on Gene Expression of Atrial Natriuretic Peptide and Neutral Endopeptidase in Rats with Chronic Heart Failure
185
206
EN
Ayman
Elsamanoudy
Department of Medical Biochemistry ,
Faculty of Medicine, Mansoura University, Egypt
Amr
Abbas
Department of Medical Physiology,
Faculty of Medicine, Mansoura University, Egypt
10.21608/besps.2010.36173
Background and Aim of work: The aim of the current study is to evaluate the effects<br />of acute and chronic inhibition of NEP, by ONO-9902, on ANP, and NEP gene<br />expressions, hemodynamic and renal parameters in rats with chronic heart failure<br />(CHF) following left coronary artery ligation (CAL). Methods: The study comprised<br />48 male Sprague-Dawley rats (220–240 g) which were divided into sham and CAL<br />groups. Myocardial infarction was induced by left CAL. All rats were divided into<br />untreated and orally treated with ONO-9902 (300 mg/kg/day) from the 1st to 6th week<br />after the operation At the 1st and 6th weeks after the operation, gene expression of<br />ANP and NEP, plasma ANP, cGMP, and aldosterone concentrations, urine volume,<br />Na and ANP excretion, creatinine clearance, renal cGMP generation, body and<br />organ weight were measured. Results: CAL led to sodium and water retention,<br />increased plasma level of ANP and aldosterone, in addition to increase in ANP gene<br />expression as well as decrease in renal generation of cGMP. Acute treatment of rats<br />with CAL by NEPI, at the first week after the operation, inhibited the NEP gene<br />expression with increased plasma ANP concentration and gene expression, which<br />caused diuresis and natriuresis and increased renal cGMP generation. Moreover,<br />chronic treatment by NEPI caused significant decrease in lung weight, lung body<br />weight ratio, NEP gene expression and PAC, non significant increase in plasma<br />concentration and gene expression of ANP, diuresis and natriuresis with increased<br />renal cGMP generation. GFR is not significantly changed either before or after<br />treatment. Conclusions: It is concluded that gene expression and plasma level of<br />ANP increased in CHF. Also, chronic treatment with NEP inhibitor improves<br />pulmonary edema and decreases Na and water retention in rats with CHF by<br />deceasing degradative effect of NEP on ANP which leads to prolongation of its<br />bioactivity. So, ONO-9902 may offer a new therapeutic approach in patients with<br />CHF.
Heart failure,kidney,atrial natriuretic peptide,neutral endopeptidase,aldosterone,Rats
https://besps.journals.ekb.eg/article_36173.html
https://besps.journals.ekb.eg/article_36173_d6b44d023eb62ac158a073acf4905650.pdf
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
30
1
2010
12
01
The Effect of Aspirin on Survivin and COX-2 Expression and Their Correlation in Aberrant Crypt Foci
207
220
EN
Maggie
Ramzy
Biochemistry Department,
Faculty of Medicine, Minia University
Soha
Abdelwahab
Histology Department,
Faculty of Medicine, Minia University
10.21608/besps.2010.36174
Non steroidal anti-inflammatory drugs (NSAID) such as aspirin have<br />chemopreventive activity against colorectal tumors. Although the molecular<br />mechanism has not been fully established, it is thought to involve COX-mediated<br />carcinogen activation, cell proliferation, apoptosis and immune surveillance. In the<br />present study, the effect of aspirin on the expression of survivin and COX-2 and their<br />possible correlation in induced colorectal carcinoma were investigated. We used 1, 2<br />dimethylhydrazine (DMH) for induction of aberrant crypt foci (ACF) that contribute<br />to the stepwise progression to colorectal cancer. The rats were divided into 3 groups;<br />control group, DMH group and DMH with aspirin group. The expression of survivin<br />and COX-2 was investigated through IHC and immunoblot and the correlation in<br />both DMH-induced lesions and after aspirin treatment was evaluated. It was found<br />that aspirin has the ability to reduce the incidence of the ACF when it was given as a<br />chemopreventive and it was found that both survivin and COX-2 are overexpressed in<br />ACF compared to normal group. They seem to be early events in the occurrence of<br />colorectal carcinoma. In the evaluation to the effect of aspirin it was found that<br />aspirin significantly causes down-regulation of both survivin and COX-2 (P=0.0001).<br />Furthermore, we found survivin and COX-2 to be positively correlated in ACF lesion<br />and after aspirin treatment (r = 0.816 and r = 0.742) respectively. All of these<br />findings suggested that targeted therapies against survivin and COX-2 may be<br />valuable in chemoprevention of colorectal carcinoma.
aspirin,aberrant crypt foci,survivin,Cyclooxygenase-2
https://besps.journals.ekb.eg/article_36174.html
https://besps.journals.ekb.eg/article_36174_32d5c344fcdf713fd006b302df72b5b9.pdf
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
30
1
2010
12
01
Protective Effect of Nigella Sativa in Alloxan Induced Diabetic Rats
221
232
EN
Ahmed
Mahmoud
Biochemistry Department, Faculty of Medicine, Minia University
ahmed_physiology@yahoo.com
Bahaa
Abdel- Salam
Zoology Department, Faculty of Science, Minia University
Wafaey
Gomaa
Pathology Department, Faculty of Medicine, Minia University
10.21608/besps.2010.36175
Oxidative stress has been suggested to be a contributory factor in complication of<br />diabetes mellitus. There are many reports indicating the change in parameter of<br />oxidative stress in alloxan induced diabetic rats, we aimed to find if the oxidative<br />stress which occurs in pancreas has a role in development of diabetes mellitus. This<br />was done by measuring plasma lipid profile, insulin, interleukin 8 (IL-8),<br />transforming growth factor α and reduced glutathione content in pancreas. The study<br />was carried on 30 rats, classified into 3 groups: control group, diabetic group in<br />which diabetes was induced by a single intraperitoneal (i.p) injection of alloxan<br />monohydrate (120 mg/kg of body weight), treated group in which rats were given<br />Nigella sativa extract i.p. (0.2 ml/kg/day), 2 week before injection of alloxan, these<br />injections were continued daily until the end of the study (for 4 weeks). The results<br />showed significant increase of plasma glucose, total cholesterol, triglycerides, low<br />density lipoprotein cholesterol (LDLc), interleukin 8 and transforming growth factor<br />α in diabetic group, with significant decrease in high density lipoprotein cholesterol<br />(HDLc), reduced glutathione content in pancreas, and insulin level in diabetic group.<br />The treated groups showed significant reduction in plasma glucose, total cholesterol,<br />triglycerides, LDLc, interleukin 8 and transforming growth factor α with significant<br />increase in HDLc, reduced glutathione content in pancreas and insulin level. It could<br />be concluded that Nigella sativa treatment for 1.5 months in rats with experimentally<br />alloxan-induced diabetes increase the activity of the anti-oxidant defense system.<br />Thus, it could be used as an anti-diabetic complement in cases of diabetes mellitus.
Alloxan and Nigella Sativa in rats
https://besps.journals.ekb.eg/article_36175.html
https://besps.journals.ekb.eg/article_36175_bb6c56332e2725b4b8a2739b9d416512.pdf
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
30
1
2010
12
01
Protective Effect of Melatonin on Carbon Tetrachloride - induced Hepatic Fibrogenesis in Rats
233
254
EN
Mona
El-Karn
Medical Physiology Department, Faculty of Medicine, Assiut University
10.21608/besps.2010.36176
Background: Liver cirrhosis is a critical stage of chronic liver diseases that can<br />produce liver failure, portal hypertension and hepatic carcinoma. Sustained oxidative<br />stress plays a key role in cell damage and fibrosis induced during liver cirrhosis. Aim<br />of the work: The aim of the present study was to examine the potential protective<br />effect of exogenous melatonin co-treatment on liver tissue injury and oxidative stress<br />processes during induction of early phase of liver fibrosis by carbon tetrachloride<br />(CCl4) injection in rats. Methods: Hepatic fibrogenesis model was induced in this<br />study by subcutaneous injection of rats by carbon tetrachloride (CCl4). Eighteen<br />adult, female albino rats were randomly divided into 3 groups (n = 6): control group<br />(group I), carbon tetrachloride treated group (group II) and CCl4 + melatonin cotreated<br />group (group III). Rats in CCl4 treated group were injected subcutaneously<br />with sterile CCl4 (2 ml/kg of body weight) in a ratio of 1:1 with olive oil twice a week<br />for 8 weeks. Rats of group III (melatonin co-treated group) were injected with CCl4 in<br />the same manner as in group II and received intraperitoneal melatonin injection in a<br />dose of 20 mg/kg twice a week for 8 weeks, starting from the beginning of CCl4<br />injection. Rats in normal control group were injected subcutaneously with olive oil at<br />the same dose and frequency as those in CCl4 treated group. At the end of the<br />experiment, rats were sacrificed, blood samples were collected for biochemical assay.<br />Liver from each animal was removed for histopathological examination.<br />Measurement of oxidative stress markers in serum was done by chemical estimation<br />of serum levels of free radicals: lipid peroxides (LPO) and nitric oxide (NO).<br />Antioxidant enzymes were estimated by chemical measurement of glutathione<br />peroxidase (GSH-Px) and superoxide dismutase (SOD) in the serum. Liver injury was<br />assessed by evaluation of serum levels of liver enzymes (alanine aminotransferase<br />(ALT), and aspartate aminotransferase (AST)). Determination of development of<br />early phase of hepatic fibrogenesis was done by chemical measurement of serum level<br />of hyaluronic acid (HA) using enzyme immunoassay (ELISA), and by<br />histopathological examinations of hepatic tissues to detect early fibrotic changes as<br />well as other histological damage of hepatic tissue caused by CCl4 injection with or<br />without melatonin administration. Results: Results of the present study showed that<br />CCl4 treatment to rats of group II caused highly significant increase in serum levels<br />of oxidative stress markers (lipid peroxides and nitric oxide), decrease in serum levels<br />of antioxidant markers (glutathione peroxidase and superoxide dismutase), increase<br />of serum levels of hepatic enzymes (ALT and AST) as well as increased serum level of<br />hyaluronic acid (HA) 8 weeks after CCl4 injections when compared with control<br />group. Melatonin co-treatment to animals of group III caused significant reduction in
serum levels of lipid peroxides (LPS) and nitric oxide (NO), significant increase in<br />plasma levels of glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD),<br />significant reduction in serum levels of liver enzymes (ALT and AST) as well as<br />significant decrease in serum level of hyaluronic acid (HA) 8 weeks after CCl4<br />injections when compared with group II. Histopathological study of liver tissue of<br />animals of CCl4 treated group showed various manifestation of hepatic cell damage<br />and early phase of fibrogenesis as necrosis, degeneration, collagen deposition and<br />few fibrous threads extending into the hepatic lobules. Histopathological study of<br />hepatic tissue of melatonin co-treated group showed that melatonin caused marked<br />amelioration of histological manifestations of hepatic cell degeneration and absence<br />of any sign of fibrogenesis with nearly normalization of the histological appearance<br />of the hepatic tissue. Compared with CCl4 treated group (group II), histological<br />appearance of hepatic tissue of rats in melatonin co-treated group (group III) showed<br />significant improvement. Conclusion: Results of this study suggest that melatonin has<br />a substantial hepatoprotective effect in a rat hepatic fibrosis model induced by an 8-<br />weeks’ CCl4 regimen. The protective effect of melatonin may be due to both its direct<br />radical scavenging properties and indirect effect as a regulator of antioxidant<br />systems. Therefore, the study proposes that melatonin may be a valuable drug for<br />inhibition of unwanted fibrosis in patients exposed to different hepatotoxic agents.
https://besps.journals.ekb.eg/article_36176.html
https://besps.journals.ekb.eg/article_36176_01a6af77d0ce0c6574edcfe17a3abab6.pdf
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
30
1
2010
12
01
Impact of Obesity in School Age of Late Childhood in Helwan Governorate
255
274
EN
Afaf
Mohsen
Community Health Nursing,, Faculty of Nursing –Helwan University
Safaa
Ismail
Pediatric Health Nursing, Faculty of Nursing –Helwan University
10.21608/besps.2010.36177
Background: Childhood obesity is an emerging global public health challenge<br />because of the great increase in the prevalence of obesity among children and<br />adolescents in all parts of the world. In Egypt, the prevalence of obesity among<br />children and adolescent was 14.7 %and 15.08 %for males and females respectively.<br />In addition, the incidence of overweight and obesity among children is slowly<br />becoming a world-wide problem in developed and developing countries .There is no<br />doubt that the percentages are even greater nowadays because of physical inactivity<br />and westernization in diet. In all times and in all cases, prevention is better than cure.<br />So, health care providers need to take a proactive role when treating children and<br />focus on prevention of obesity rather than waiting until the condition exists. Aim of<br />study: is to assess life style habits which lead to obesity for school age children and<br />evaluate its effect on puberty in school age children. Method: This is a descriptive<br />research design; it was carried out in Ezbet Elwalda in Helwan governorate. Subjects<br />were all obese students and accepted to participate in the study from both sexes,<br />aged10- 12 years old ( late childhood stage), 13- 17 years old ( the adolescent stage)<br />and Marley juvenile diabetes were excluded and their total number was 216.The<br />researchers utilized self administered interview sheet which included the following ,<br />personal information, eating habits, life style pattern and gender puberty and<br />physical assessment (height , weight & BMI).All data collected from first of March to<br />the end of April 2010. Results: The majority of studied sample had boys, their<br />numbers was 138 from 216 student. The majority of studied sample were prefer to eat<br />fast food on weekly basis (41.7%), drink beverage and eat candy (70.8%), take snacks<br />between meals (52.8%) as ice cream in (66.7%) and eat potato chips (60.2%). Three<br />quarter of obese sample had not seeking medical advice and 71.4 % were physically<br />inactivity. All obese girls had pubic hair, armpit hair, developed nipple of the breast<br />and 40 % of them started menarche at eleven years old. Otherwise there is no<br />significant relation between overweight and obese boys regarding developed testes,<br />emergence of armpit hair, developed growth hand and foot, masculine voice.<br />Recommendation: Health care providers need to play a preventive role when<br />treating children and focus on prevention of obesity rather than wait until the<br />condition exists. Also, referring client to an experienced registered dietitian to obtain<br />a full nutritional assessment for the child and family.
obese,overweight,BMI
https://besps.journals.ekb.eg/article_36177.html
https://besps.journals.ekb.eg/article_36177_1feadee6478102ee7e36e6e794067285.pdf
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
30
1
2010
12
01
A Study of the Role of Some Antioxidants in Diabetes Induced in Rats
275
286
EN
Nagy
Tawfek
Department of Zoology, Faculty of Science , El-Minia University, El-Minia, Egypt
Mahmoud
Elrehany
Departement of
Biochemistry, Faculty of Medicine, El-Minia University, El-Minia, Egypt
Adel
Basyouny
Department of Zoology, Faculty of Science , El-Minia University, El-Minia, Egypt
Hanaa
Hassan
Department of Zoology, Faculty of Science , El-Minia University, El-Minia, Egypt
10.21608/besps.2010.36178
Oxidative stress is produced under diabetic conditions and possibly causes various<br />forms of tissue damage and destruction of pancreatic β-cells in insulin-dependent<br />diabetes mellitus (IDDM) patients. The present study was carried out to examine the<br />involvement of oxidative stress in the progression of pancreatic β-cell dysfunction in<br />type 1 diabetes and to evaluate the potential usefulness of antioxidants in the<br />treatment of type 1diabetes. The present study was achieved using ٢٤ male Sprague<br />Dawley albino rats. Rats were divided into three groups: normal control rats,<br />diabetic control rats, and diabetic rats received mixture of antioxidants. A mixture of<br />antioxidants (N-acetyl-cysteine (NAC), alpha-lipoic acid (LA), vitamin E and vitamin<br />C) was orally administered daily to cyclophosphamide-induced diabetic rats for a<br />period of two months. The results revealed that these antioxidants exerted<br />amelioration in fasting plasma glucose level, significant inhibition of lipid peroxides<br />level and observed elevation in glutathione (GSH) level and glutathione peroxidase<br />(GPX) activity of diabetic rats. On the basis of the present results it could be<br />concluded that (N-acetyl-cysteine (NAC), alpha-lipoic (LA), vitamin E and vitamin C)<br />restored the activities of the studied parameters and the activities of some enzymes in<br />different ways, depending on special mechanism in each one. Supplementation of<br />antioxidants at once after diagnosis of diabetes may delay the complications of<br />diabetes. This finding suggests a potential usefulness of antioxidants for treating<br />diabetes and provides further support for the implication of oxidative stress in β-cell<br />dysfunction in diabetes.
https://besps.journals.ekb.eg/article_36178.html
https://besps.journals.ekb.eg/article_36178_e2920d55e887f014de4824a7de8c5b39.pdf
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
30
1
2010
12
01
Role of N-Acetylcystein in Protection against Cisplatin Nephrotoxicity
287
300
EN
Sahar
Habib
Forensic Med. And Toxicology Department,
Faculty of Medicine, Minia University
Maggie
Ramzy
Biochemistry Department,
Faculty of Medicine, Minia University
Manal
Abd Elghany
Pathology Department,
Faculty of Medicine, Minia University
10.21608/besps.2010.36179
Background: Cisplatin (CDDP) is a cytotoxic therapeutic agent that causes many<br />physiological adverse effects such as nephrotoxicity. Although N-acetylcystein (NAC)<br />a thiol containing antioxidant, has been documented to be effective in attenuating<br />CDDP induced renal injury, the precise mechanisms involved in its renoprotection<br />have not been completely clarified. Methods: Four groups of albino rats were used.<br />The first group injected by saline as control, the second by NAC (i.p.), the third by a<br />single bolus of cisplatin (i.v.), and the last by NAC/CDDP, after 5 days from CDDP<br />injection, investigations were conducted on the levels of serum urea and creatinine,<br />oxidant/antioxidant status by estimation of malondialdehyde, catalase and reduced<br />glutathione, and renal and systemic tumor necrosis factor-alpha (TNF-α). Also, renal<br />tissues were examined histopathologically. Results: Administration of cisplatin to rats<br />induced a significant increase in serum urea and creatinine levels in addition to<br />severe alterations in renal tissue architecture. Cisplatin also increased<br />malondialdehyde and decreased glutathione, and catalase in renal tissues. Also,<br />CDDP increased both renal and systemic TNF-α. Administration of NAC markedly<br />reduced the cisplatin-induced higher serum creatinine and urea levels and<br />counteracted the effects of cisplatin on oxidative stress markers, protected the tissues<br />from the cisplatin-induced lipid peroxidation, and increased TNF-α and improved<br />renal tissue architecture. Conclusion: NAC protection is mediated by preventing the<br />decline of antioxidant status, inhibit malondialdehyde and TNF-α and prevent<br />necrosis and apoptosis from kidneys. These results have implications in use of NAC in<br />human application for protecting against drug-induced nephrotoxicity.
Cisplatin,Nephrotoxicity,reduced glutathione,Malondialdehyde,catalase,NAC,TNF- α,apoptosis
https://besps.journals.ekb.eg/article_36179.html
https://besps.journals.ekb.eg/article_36179_23923bfc4e05142356f45b018e6fad25.pdf
Egyptian Society for Physiological Sciences
Bulletin of Egyptian Society for Physiological Sciences
1110-0842
2356-9514
30
1
2010
12
01
Bcl2 and c-erbB-3 as Prognostic Markers of Hepatocellular Carcinoma
301
310
EN
Hanan
Fouad
Medical Biochemistry Department, Faculty of Medicine, Cairo University
Ahmed
Eldemery
Biochemistry Department, Faculty of Medicine, October 6 University
10.21608/besps.2010.36180
The present study was conducted to evaluate gene expression profile of c-erbB-3 and<br />bcl2 in hepatocellular carcinoma patients (HCC) with and without associated<br />hepatitis C virus (HCV) infection. Forty eight subjects were included in the study and<br />divided equally into 2 groups: Group 1 involved HCC subjects with associated HCV<br />infection and group 2 involved HCC subjects without HCV associated infection.<br />Adjacent para-cancerous tissues were examined as control samples. Correlations<br />with various clinico-pathological parameters of the tumor were assessed; stage,<br />tumor size, intra-hepatic metastasis and carcinoma differentiation. c-erbB-3<br />oncogene was expressed in 83.33% (40/48) of total HCC samples and in 31.25%<br />(15/48) of the noncancerous lesions. c-erbB-3 was expressed in 87.5% (21/24) of<br />HCC samples with associated HCV infection and in 79.16% (19/24) of HCC without<br />associated HCV infection. Gene expression of c-erbB-3 was significantly correlated<br />to the clinico-pathological parameters of the tumor. As regards bcl2 gene expression,<br />the gene was expressed in 20.8% (10/48) of total HCC samples and in the paracancerous<br />lesions. Bcl2 was expressed in 20.8% (5/24) of HCC samples with and<br />without HCV associated infection. Gene expression of bcl2 did not show significant<br />correlations to the clinico-pathological parameters of the tumor. In conclusion,<br />expression profile of c-erbB-3 in hepatocellular carcinoma patients could be used as<br />a prognostic molecular marker in HCC.
Hepatocellular carcinoma,Bcl2,c-erbB-3
https://besps.journals.ekb.eg/article_36180.html
https://besps.journals.ekb.eg/article_36180_2e6aa55c1e7204435881aca355c756dd.pdf