Possible role of angiogenesis suppression in rats model of non alcoholic fatty liver disease

Abdel Ghaffar Rezk, Dalia Mohammed; Sarhan, Mohammed El Mahdy; Abdel Moniem, Hanaa Ahmed; Abbas, Amr Medhat; Salama, Mohammad Eldosoky

doi:10.21608/besps.2019.4410.1004

Possible role of angiogenesis suppression in rats model of non alcoholic fatty liver disease

Document Type : Original Article

Authors

¹ physiology department, faculty of medicine, mansoura university, talkha city, dakahlyia gov.

² physiology depatment, Faculty of Medicine, Mansoura University, Masoura, Egypt.

³ physiology department, faculty of medicine, mansoura university, mansoura, eygpt

⁴ physiology department, faculty of medicine, mansoura university, mansoura, eygpt.

10.21608/besps.2019.4410.1004

Abstract

Objective:to evaluate development of angiogenesis in rats of NAFLD and to determine the protective effects of antiangiogenic therapy (sorafenib) in preventing the progression of NAFLD.
Methods: 45 Albino rats (200-300 g) were divided into 3 groups (15 rats for each): Group I: Control group fed on an ordinary diet. Group II: rats received high fat, high fructose diet (HFD,HFr) with DEN ( twice weekly for 8 weeks, ip). Group III: rats received HFD, HFr + DEN + sorafenib orally for 8 weeks. Biochemical, histopathologial, and immunohistopathological examination were studied.
Results: high fat, high fructose diet with DEN resulted in a significant elevation in the serum cholesterol, TG, LDL, and AST, and ALT, significantly lower levels of HDL and Albumin together with a significant decrease in hepatic GSH, Histopathological examination revealed that liver of untreated rats showed severe fatty infilteration (grade3). Immunohistochemical examination of liver of untreated NAFLD rats showed strong staining reactions against VEGF, α- SMA, CD31, and Caspase3 antibodies. Oral administration of sorafenib alleviated all these parameters.

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