Document Type : Original Article
Authors
1
Department of Physiology, Faculty of Medicine, Al-Azhar University, Assuit, Egypt.
2
Department of Physiology, Faculty of Medicine, Al-Azhar University, Cairo, Egypt.
3
Department of clinical Pathology, Faculty of Medicine, Al-Azhar University, Assuit, Egypt
4
Department of clinical Pathology, Faculty of Medicine, Al-Azhar University, Assuit, Egypt,
5
Department of haematology , Theodor Bilharze,Research institute, Cairo, Egypt
Abstract
Background: Immobilization stress known to stimulate sympathetic activity, as well as the hypothalamic–pituitary–adrenal axis (HPA), produces a significant increase in adrenomedullin (AdM) levels, suggesting a regulatory or protective role for AdM in countering HPA activation that follows a variety of stressors. Stressors can modulate the secretion of proinflammatory cytokines. Interleukin (IL)-10 is a potent activator of the HPA and appears to play a pathogenic role in conditions related to stress Objective: The aim of this study was to evaluate and validate the effects of AdM administration and immobilization stress treatment on IL-10 levels in rat liver, lung, brain and heart tissues. Materials and Methods: The study was carried out on twenty-four male albino rats (8 months old, 190–240 g). The animals were divided into 4 groups of 6 rats each group. Group A : control group. Group B : AdM-treated group , rats received intraperitonealy (i.p.) injection of AdM (2000 μg/g body weight) once daily for 1 week. Group C : immobilized stress group ,(rats were immobilized by keeping them into transparent plastic jars with 5 holes for 4hrs per day for 1 week).Group D: immobilized stress +AdM group. Rats were immobilized by keeping them into transparent plastic jars with 5 holes for 4hrs a day for 1 week and were given AdM i.p at a single dose of 2000 μg/g body weight for a week. At the end of the experiment, the concentration of IL-10 was determined using an enzyme-linked immunosorbent assay (ELISA) kit. Results: The results of the present study showed that IL-10 levels increased in all tissues in immobilized stress group when compared to control , also IL-10 levels were increased in AdM treatment group in all tissues when compared to control . IL-10 levels were decreased in the immobilization stress + AdM treatment group in all tissues when compared to the stress group, increased IL-10 levels in brain and lung tissue but decreased in heart and liver tissues in the immobilization stress + AdM treatment group when compared to the AdM treatment group. Conclusion: The results suggest that immobilization stress may induce increase of rat proinflammatory cytokine IL-10 and AdM may play a regulatory or protective role for immobilization stress.
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