Document Type : Review Article
Authors
1
Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Benha University, Benha 13518, Egypt.
2
Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh 13736, Qalyubia, Egypt.
3
Department of Physiology, Faculty of Medicine, Benha University, Benha 13518, Egypt.
4
Department of Histology and Cell Biology, Faculty of Medicine, Benha University, Benha 13518, Egypt.
5
Deparment of Pharmacology, Faculty of Medicine, Benha University, Benha 13518, Egypt.
10.21608/besps.2024.279336.1166
Abstract
Background: Type 2 diabetes mellitus (T2DM), which affects millions of individuals, has become a serious health problem. The mammalian target of rapamycin (mTOR) hyperactivation is a negatively involved autophagy that may in fact aid in the loss of β-cell function. Resveratrol (RSV) is a naturally occurring phytoalexin, mostly found in cereals, fruits, and vegetables that have antioxidant effects.
Aim: In the current work, Wistar rats exposed to high-fat diet (HFD) and streptozotocin (STZ)-induced T2DM were evaluated for resveratrol's protective effects on pancreatic functions, and mTOR-mediated autophagy.
Materials and Methods: Four equal groups of forty male Wistar rats were created at random. Group 1 (G1) was maintained in typical control circumstances and given a balanced diet; G2, G3, and G4 were fed HFD for 10 weeks continuously; at the end of the 6th week injected I/P STZ as a single dose to induce T2DM. Three days after diabetic induction, G3 was received RSV daily for continuously 4 weeks. G4 was received RSV in addition to Chloroquine (CQ) as autophagy inhibitor.
Results: The findings shown that RSV reduces the pancreatic dysfunctions brought on by HFD-STZ by inhibiting the mTOR pathway through decreasing the level of mTOR, Cas-3, and p62 and increasing the level of LC3. RSV activate autophagy and inhibit apoptotic-mediated cell death confirmed by transmission electron micrograph findings.
Conclusion: RSV is thought to protect pancreatic cells by inhibiting the mTOR pathway, which regulates the transition between autophagy and apoptotic machinery in diabetic circumstances.
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