Histophysiological Evaluation of the Effect of Alpha-Tocopherol and Dimethyl Diphenyl Bicarboxylate on Arsenic-Induced Hepatotoxicity in Adult Male Albino Rats

Document Type : Original Article

Authors

1 Department of Histology, Faculty of Medicine, El-Minya University

2 Department of Physiology, Faculty of Medicine, Tanta University

3 Department of Forensic & Toxicology, Faculty of Medicine, El-Minya University, Egypt

Abstract

Arsenic, a widely studied medicinal and toxicological element, is known to induce
oxidative stress and damage to cells. The present study was aimed at to assess the
effect of vitamin E (alpha-tocopherol) and/or DDB (dimethyl diphenyl bicarboxylate)
with or without the chelator DMSA (Meso 2,3-dimercaptosuccinic acid) on arsenic-induced hepatotoxicity. Fifty four adult malealbino rats were divided into nine
groups. Group I was the control and received only intraperitoneal injection normal
saline 2 times per week for two weeks. Group II was injected with sodium arsenite in
normal saline 2 times /week for 2 weeks. Group III was injected with sodium arsenite
and received oral DMSA daily for 2 weeks. Group IV was injected with sodium
arsenite and received oral vitamin E (alpha –tocopherol) daily for 2 weeks. Group V
was injected with sodium arsenite and received oral DDB daily for 2 weeks. Group VI
received sodium arsenite, vitamin E and DDB for 2 weeks. Group VII received
sodium arsenite, DMSA and vitamin E for 2 weeks. Group VIII received sodium
arsenite, DMSA and DDB for 2 weeks. Group IX received sodium arsenite, DMSA,
vitamin E and DDB for 2 weeks. Physiological and biochemical parameters were
undertaken to measure Alanine Amino-Transferase (ALT), Aspartate Amino-Transferase (AST), Malondialdehyde (MDA), reduced Glutathione (GSH) and
Glutathione Peroxidase (GPx). Histochemical and histpathological parameters were
also undertaken to assess the structural-functional mirror changes. The results of this
study showed that vitamin E and DDB were almost similar in their antioxidant
hepatoprotective effects with stronger inhibiting action of DDB to lipid peroxidation
while the greatest effect was achieved by their combination witha chelating agent
like DMSA with restoration of almost the normal hepatic histology.

Files

Share

How to cite

Statistics