Document Type : Original Article
Authors
1
Medical Physiology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt
2
Anatomy and Embryology Department, Faculty of Medicine, Menoufia University, Shebin El-Kom, Menoufia, Egypt
3
Pathology Department, Faculty of Medicine, Suez Canal University, Egypt
4
Internal Medicine Department, Endocrinology Unit, Faculty of Medicine, Menoufia University, Shebin El-Kom, Menoufia, Egypt.
5
Clinical Pharmacology Department, Faculty of medicine, Damitta University, Egypt
6
Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt
7
Histology Department, Faculty of Medicine, Helwan University Egypt.
8
Physiology Department, Badr University in Cairo (BUC), Cairo, Egypt
Abstract
Background: The most prevalent kind of diabetes is type 2 diabetes mellitus (T2DM). Vascular impairment is one of the numerous problems that frequently accompany diabetes mellitus. Renin inhibitors like Aliskiren have been shown to effectively protect organs through a variety of methods.
Objective: to illustrate aliskiren's vascular-protective effects in diabetic rats and any possible underlying processes at play.
Material and methods: Thirty male albino rats were split into three groups: diabetic, diabetic+aliskiren, and control (10/group). Serum glucose, insulin, HOMA-IR index, glycosylated Hb A1c, cholesterol, triglycerides, renin activity, aortic MDA, aortic SOD, aortic TNF-α, aortic IL-6, aortic IL-10, aortic NF-kB gene expression, and aortic ENOS gene expression were all evaluated. Additionally, aortic histology and immunohistochemical analyses were performed.
Results: In contrast to the control group, the diabetic group's aortic SOD, aortic IL-10, and aortic gene expression of ENOS were significantly lower, while the measured serum glucose, insulin, HOMA-IR index, serum glycosylated Hb A1c, serum cholesterol, serum triglyceride, serum renin activity, aortic MDA, aortic TNF-α, aortic IL-6, and aortic gene expression of NF-kB were all significantly higher in the diabetic group. Additionally, as compared to the control group, the diabetic group's aortic ENOS was significantly downregulated and their aortic NF-kB immunoreaction was elevated. Aliskiren considerably reduced the vascular alterations brought on by diabetes.
Conclusion: By blocking renin activity and exhibiting hypoglycemic, lipid-lowering, anti-inflammatory, and antioxidant effects in addition to upregulating aortic ENOS, aliskiren guards against diabetes-induced vascular damage.
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