Effect of n-acetyl cysteine and moringa oleifera on acute kidney injury induced by cisplatin in rats.

Abd Elhameed, Eman Mahmoud; Halim, Zeinab; Adel, Mohamed; Abd El-Hamed, Aya E.; Messiha, Refka Khalil

doi:10.21608/besps.2025.383383.1214

Effect of n-acetyl cysteine and moringa oleifera on acute kidney injury induced by cisplatin in rats.

Document Type : Original Article

Authors

¹ Medical Physiology Department, Faculty of Medicine, Mansoura University, Egypt

² Medical Experimental Research Center (MERC), Mansoura University,Mansoura, Egypt

10.21608/besps.2025.383383.1214

Abstract

This study aimed to assess the effects of N-acetylcysteine and Moringa oleifera on cisplatin-induced acute kidney injury (AKI) in rats. Forty adult male rats were divided into five groups: Group 1 (control), Group 2 (cisplatin, 6 mg/kg IP on the 8th day), Group 3 (N-acetylcysteine, 200 mg/kg/day orally for 12 days + cisplatin), Group 4 (Moringa oleifera, 300 mg/kg/day orally for 12 days + cisplatin), and Group 5 (combined N-acetylcysteine and Moringa oleifera + cisplatin). Blood samples were collected for biochemical analysis, and renal tissue was examined for oxidative stress markers, caspase 3, and aquaporin 2 expression. Results revealed marked histopathological damage and increased caspase 3 expression in Group 2. N-acetylcysteine and Moringa oleifera treatments reduced this damage, with the best outcome in Group 5. Aquaporin 2 expression decreased significantly in Group 2 but improved in the treated groups, particularly Group 5. Serum creatinine, urea, and potassium levels increased significantly in Group 2, whereas treatment with N-acetylcysteine and Moringa oleifera significantly reduced these levels, with the best results in Group 5. Creatinine clearance decreased in Group 2 but improved with treatment, especially in Group 5. Serum KIM-1, serum NGAL, and urine NGAL were elevated in Group 2 and reduced in the treated groups, with the best outcome in Group 5. Oxidative stress markers improved in Groups 3, 4, and 5, with the best results in Group 5. A negative correlation was observed between serum KIM-1 and aquaporin 2. In conclusion, N-acetylcysteine and Moringa oleifera may protect against cisplatin-induced AKI.

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