Effect of the combination between empagliflozin and calcipotriol on cadmium-induced testicular toxicity in rats

Borg, Hany Mohammad; Kabel, Ahmed; Estfanous, Remon; Abd Elmaaboud, Maaly

doi:10.21608/besps.2019.14918.1029

Effect of the combination between empagliflozin and calcipotriol on cadmium-induced testicular toxicity in rats

Document Type : Original Article

Authors

¹ faculty of medicine, kafrelsheikh university

² Department of Pharmacology, Faculty of Medicine, Tanta University, Tanta, Egypt

³ Physiology department, Faculty of Medicine, Kafrelsheikh University, Egypt

⁴ Anatomy and Embryology Department, Faculty of Medicine, Tanta University, Tanta, Egypt

10.21608/besps.2019.14918.1029

Abstract

The aim of this study was to assess the effect of empagliflozin and/or calcipotriol on cadmium-induced testicular toxicity in rats. Sixty male Wistar rats were divided into 6 equal groups: Control; cadmium; cadmium + empagliflozin; cadmium + calcipotriol; cadmium + Carboxymethyl cellulose and cadmium + empagliflozin + calcipotriol. Testicular enzymes, serum testosterone, luteinizing hormone and follicle stimulating hormone were measured. Also, testicular tissue antioxidant enzymes, interleukin-6 (IL-6), transforming growth factor beta 1 (TGF-β1) and sperm characteristics were determined. Parts of the testes were subjected to histopathological and immunohistochemical examination. Empagliflozin/calcipotriol combination restored the testicular enzymes, sperm characteristics, hormonal profile and the antioxidant defenses compared to the use of each of these drugs alone. Also, this combination significantly ameliorated the inflammatory processes and induced significant improvement of the histopathological, and immunohistochemical picture compared to the use of each of these drugs alone. So, empagliflozin/calcipotriol combination might represent a promising therapeutic modality for amelioration of cadmium-induced testicular toxicity.

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