Gestational Diabetes Mellitus And Leptin Gene polymorphism In Egyptian Women

Abdu Allah, Azza M.; Abd El Gayed1, Eman Masoud; Abd El Gayed, Alaa Masoud; Desouky, Maha Khalaf

doi:10.21608/besps.2017.8263

Gestational Diabetes Mellitus And Leptin Gene polymorphism In Egyptian Women

Document Type : Original Article

Authors

¹ Department of Medical Biochemistry, Faculty of Medicine and National Liver Institute, Menoufia University, Egypt.

² Medical Biochemistry Department Faculty of Medicine, Menoufia University Egypt.

³ Obstetrics and Gynaecology Department Faculty of Medicine, Menoufia University Egypt.

⁴ Anatomy Department Minia University Egypt and Taibah University KSA.

10.21608/besps.2017.8263

Abstract

Gestational diabetes is blood sugar elevation during pregnancy and exposes women to the risk for development of type 2 diabetes mellitus in later years of life. The LEP rs7799039 (2548G/A) polymorphism in the 5´ region of the LEP gene was reported not only to be associated with overweight but also to have a strong influence on leptin gene expression and adipose tissue secretion. The aim of this study is to evaluate the distribution of LEP rs7799039 (2548G/A) polymorphism and its plasma level in patients with gestational Diabetes Mellitus. This study was carried out on160 subjects divided into 2 groups; 80 patients with GDM (group I) and 80 healthy subjects served as controls (group II). All studied subjects were submitted to full history taking, general clinical examination and laboratory investigations including fasting and 2 hour post prandial blood glucose, total cholesterol (TC),triglycerides (TG), HDLc, LDLc, glycated hemoglobin (HbA1c), plasma leptin, fasting serum insulin, HOMA- IR and Genotyping of LEP rs 7799039 (2548G/A) polymorphism was analyzed by the TaqMan allelic Discrimination Assay Technique. Results showed significant statistical differences between the two studied groups regarding family history and BMI. Significant statistical difference between group (I) and group (II) regarding plasma leptin level (P value <0.001), serum insulin (P value <0.001), insulin resistance (P value <0.001) and LEP rs 7799039 (2548G/A) genotype distribution with increased frequency of the AG and AA genotype in patients with gestational diabetes (P value <0.001) and increased frequency of GG genotype in controls. Conclusion: This results indicate that leptin plays a role in increasing insulin sensitivity, LEP rs7799039 (2548G/A) and its plasma level can contribute to susceptibility to GDM and might be used for screening of the early detection of GDM.

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