Serum Levels of Tumor Necrosis Factor-alpha, Nitric oxide and Malondialdehyde in Patients with Behcet’s Disease

Document Type : Original Article

Authors

1 Medical Biochemistry Department, Research Institute of Ophthalmology

2 Clinical and Chemical Pathology Department, Research Institute of Ophthalmology

3 Ophthalmology Department, Research Institute of Ophthalmology

Abstract

Objective:To determine the serum levels of tumour necrosis factor-alpha (TNF),
nitric oxide (NO) and Malondialdehyde (MDA) in patients with Behcet’s disease
(BD). This study included 27 patients with Behcet's disease and 16 healthy control
subjects. Serum (TNF-) was measured by an enzyme linked immunosorbent assay
(ELISA) while serum NO oxide levels were determined by Griess reaction. The MDA
levels were detected by thiobarbituric acidreaction. There was a significant increase
in the levels of TNF-, NO and MDA in Behcet's disease patients compared to
controls. No significant correlation was detected between TNF-and NO or MDA
levels in patients or controls. A significantpositive correlation was detected between
serum levels of NO and MDA in BD patients.This suggests that elevated levels of
TNF-, NO and MDA may be related to the pathogenesis of Behcet's disease.

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